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  4. Recombinant Arabidopsis thaliana Histone-lysine N-methyltransferase ASHH2 (ASHH2), partial

Recombinant Arabidopsis thaliana Histone-lysine N-methyltransferase ASHH2 (ASHH2), partial

  • 中文名稱：
    Recombinant Arabidopsis thaliana Histone-lysine N-methyltransferase ASHH2(ASHH2) ,partial
  • 貨號：
    CSB-YP645694DOA
  • 說明書：
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Arabidopsis thaliana Histone-lysine N-methyltransferase ASHH2(ASHH2) ,partial
  • 貨號：
    CSB-EP645694DOA
  • 說明書：
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Arabidopsis thaliana Histone-lysine N-methyltransferase ASHH2(ASHH2) ,partial
  • 貨號：
    CSB-EP645694DOA-B
  • 說明書：
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Arabidopsis thaliana Histone-lysine N-methyltransferase ASHH2(ASHH2) ,partial
  • 貨號：
    CSB-BP645694DOA
  • 說明書：
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Arabidopsis thaliana Histone-lysine N-methyltransferase ASHH2(ASHH2) ,partial
  • 貨號：
    CSB-MP645694DOA
  • 說明書：
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    ASHH2
  • Uniprot No.：
  • 別名：
    ASHH2; EFS; LAZ2; SDG8; SET8; At1g77300; T14N5.15Histone-lysine N-methyltransferase ASHH2; EC 2.1.1.-; ASH1 homolog 2; H3-K4-HMTase; Histone H3-K36 methyltransferase 8; H3-K36-HMTase 8; Protein EARLY FLOWERING IN SHORT DAYS; Protein LAZARUS 2; Protein SET DOMAIN GROUP 8
  • 種屬：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Histone methyltransferase involved in di and tri-methylation of 'Lys-36' of histone H3 (H3K36me2 and H3K36me3). Binds to H3 already mono- or di-methylated on 'Lys-4'(H3K4me1 or H3K4me2), but not to H3K4me3. H3K4me and H3K36me represent specific tags for epigenetic transcriptional activation. Regulates positively FLC transcription to prevent early flowering transition. Required for flowering transition in response to vernalization and for the maintenance of FLC expression in late embryos, but dispensable for the initial reactivation in early embryos during reprogramming. Seems also to modulate several traits including floral organ size, root size and dormancy. Promotes apical dominance. Directly involved in the tri-methylation of 'Lys-36' of histone H3 (H3K36me3) at LAZ5 chromatin to maintain a transcriptionally active state of LAZ5, a TIR-NB-LRR protein involved in innate immunity. Required for brassinosteroid (BR)-induced gene expression and histone H3 trimethylation on 'Lys-36' (H3K36me3) in BR-regulated genes.
  • 基因功能參考文獻：
    1. findings provide first structural insights into the molecular basis for specific recognition of monomethylated H3K4 by the H3K4me1 reader protein SDG8 from Arabidopsis. PMID: 29496997
    2. both maternal and paternal EFS alleles are responsible for the seed size regulation PMID: 29372509
    3. SDG8 plays an essential role in mediating BR-regulated gene expression PMID: 24838002
    4. provide evidence that ASHH2 is acting on H3K4me-marked genes, allowing for ASHH2-dependent H3K36 tri-methylation, which contributes to sustained expression of tissue-specific and developmentally regulated genes. PMID: 21522130
    5. SDG8 is required for basal and R protein-mediated pathogen resistance in Arabidopsis, revealing the importance of chromatin remodeling as a key process in plant innate immunity. PMID: 20949080
    6. SDG8 plays crucial roles in plant defense against necrotrophic fungal pathogens through histone H3K36me3-mediated activation of a subset of genes within the jasmonate/ethylene pathway. PMID: 20810545
    7. These results suggest that SDG8 regulates shoot branching via controlling the methylation states of its target genes. PMID: 18602372
    8. The CCR1 gene encodes a histone methyltransferase that methylates histone H3 and downregulates CRTISO. PMID: 19174535

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  • 亞細胞定位：
    Nucleus. Chromosome, centromere.
  • 蛋白家族：
    Class V-like SAM-binding methyltransferase superfamily, Histone-lysine methyltransferase family, SET2 subfamily
  • 組織特異性：
    Ubiquitous, with higher levels in young tissues, including shoot and root apex. Expressed in ovules, tapetum layer and microspores.
  • 數(shù)據(jù)庫鏈接：

    KEGG: ath:AT1G77300

    STRING: 3702.AT1G77300.1

    UniGene: At.34505



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