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  4. Recombinant Arabidopsis thaliana Histone H3-like centromeric protein HTR12 (HTR12)

Recombinant Arabidopsis thaliana Histone H3-like centromeric protein HTR12 (HTR12)

  • 中文名稱：
    擬南芥HTR12重組蛋白
  • 貨號：
    CSB-YP840421DOA
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    擬南芥HTR12重組蛋白
  • 貨號：
    CSB-EP840421DOA
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    擬南芥HTR12重組蛋白
  • 貨號：
    CSB-EP840421DOA-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    擬南芥HTR12重組蛋白
  • 貨號：
    CSB-BP840421DOA
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    擬南芥HTR12重組蛋白
  • 貨號：
    CSB-MP840421DOA
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    HTR12
  • Uniprot No.：
  • 別名：
    HTR12; CENH3; At1g01370; F6F3.17Histone H3-like centromeric protein HTR12; Centromeric histone CENH3
  • 種屬：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白長度：
    Full Length of Mature Protein
  • 表達區域：
    2-178
  • 氨基酸序列
    ARTKHRVTR SQPRNQTDAA GASSSQAAGP TTTPTRRGGE GGDNTQQTNP TTSPATGTRR GAKRSRQAMP RGSQKKSYRY RPGTVALKEI RHFQKQTNLL IPAASFIREV RSITHMLAPP QINRWTAEAL VALQEAAEDY LVGLFSDSML CAIHARRVTL MRKDFELARR LGGKGRPW
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Histone H3-like variant which exclusively replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore. Required for recruitment and assembly of kinetochore proteins, mitotic progression and chromosome segregation. May serve as an epigenetic mark that propagates centromere identity through replication and cell division.
  • 基因功能參考文獻：
    1. Extreme divergence in CENH3 protein sequence does not alter centromere location in Arabidopsis. PMID: 28223399
    2. Reduced endogenous CENH3 and lack of meiotic incorporation of EYFP-CENH3(C) lead to reduced fertility caused by insufficient CENH3 loading to the centromeres of meiotic chromosomes, subsequent lagging of chromosomes and formation of micronuclei. PMID: 21635586
    3. CENH3 is expressed in dividing tissues and is CENH3 is transcriptionally regulated by E2F/DP transcription factors. PMID: 21771121
    4. Deposition of Arabidopsis CENH3 takes place at centromeres during G2. PMID: 21842230
    5. Meiosis-specific loading of the centromere-specific histone CENH3 in Arabidopsis thaliana PMID: 21695238
    6. study concludes that CENH3 function in A. thaliana, an organism with large tandem repeat centromeres, has stringent requirements for functional complementation in mitosis PMID: 20628040
    7. Alignment of CENH3 sequences revealed that the tested species share only three amino acids at loop1 region: threonine2, arginine12 and alanine15 PMID: 21190064
    8. cenh3 null mutants expressing altered CENH3 proteins are crossed to wild type, chromosomes from the mutant are eliminated, producing haploid progeny PMID: 20336146

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  • 亞細胞定位：
    Chromosome, centromere, kinetochore.
  • 蛋白家族：
    Histone H3 family
  • 數據庫鏈接：

    KEGG: ath:AT1G01370

    STRING: 3702.AT1G01370.1

    UniGene: At.42652



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