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  4. Recombinant Arabidopsis thaliana Fe (2+) transport protein 1 (IRT1), partial

Recombinant Arabidopsis thaliana Fe (2+) transport protein 1 (IRT1), partial

  • 中文名稱：
    擬南芥IRT1重組蛋白
  • 貨號：
    CSB-YP655032DOA
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    擬南芥IRT1重組蛋白
  • 貨號：
    CSB-EP655032DOA
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    擬南芥IRT1重組蛋白
  • 貨號：
    CSB-EP655032DOA-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    擬南芥IRT1重組蛋白
  • 貨號：
    CSB-BP655032DOA
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    擬南芥IRT1重組蛋白
  • 貨號：
    CSB-MP655032DOA
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    IRT1
  • Uniprot No.：
  • 別名：
    IRT1; At4g19690; T16H5.50; Fe(2+) transport protein 1; Fe(II) transport protein 1; Iron-regulated transporter 1
  • 種屬：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    High-affinity iron transporter that plays a key role in the uptake of iron from the rhizosphere across the plasma membrane in the root epidermal layer. Acts as the principal regulator of iron homeostasis in planta. Also mediates the heavy metals uptake under iron-deficiency by its ability to transport cobalt, cadmium, manganese and/or zinc ions.
  • 基因功能參考文獻：
    1. Data suggest that NRAMP1 plays a pivotal role in Fe transport by cooperating with IRT1 to take up Fe in roots under replete conditions. PMID: 27849020
    2. role of IRT1 in iron uptake PMID: 25778985
    3. Our results reveal a role of SNX1 for the correct trafficking of IRT1 and, thus, for modulating the activity of the iron uptake machinery PMID: 24596241
    4. This work establishes a functional link between the dynamics and the lateral polarity of IRT1 and the transport of its substrates, and identifies a molecular mechanism driving polar localization of a cell surface protein in plants. PMID: 24843126
    5. IDF1 directly regulates IRT1 degradation through its RING-type E3 ligase activity. PMID: 23995086
    6. AtIRT1 transports nicekl (Ni(2+)) in roots, and strongly suggest that Ni accumulation is further accelerated by AtIRT1 that is expressed in response to excess Ni. PMID: 21742768
    7. These data suggest a model in which monoubiquitin-dependent internalization/sorting and turnover keep the plasma membrane pool of IRT1 low to ensure proper iron uptake and to prevent metal toxicity. PMID: 21628566
    8. IRT1 and FRO2 genes are upregulated under iron starvation. PMID: 21426424
    9. Application of the lipoxygenase inhibitor ibuprofene to plants caused an up-regulation of IRT1 gene expression. PMID: 21334215
    10. Although ferrous ions and other IRT1-transported metal ions do not bind very tightly, the Histidine-rich sequence has a very high entropy-driven affinity for Fe3+, which may have biological significance. PMID: 17029360
    11. Expression of each of the IRT1 variants in plants from the 35S promoter revealed that K146 or K171 is required for iron-induced protein turnover, and 35S-IRT1K146R,K171R plants contain higher levels of iron compared to 35S-IRT1 and wild type PMID: 18305211
    12. Acidification capacity varied among Arabidopsis accessions and was associated with a high induction of AHA2 and IRT1... PMID: 19549134

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  • 亞細胞定位：
    Cell membrane; Multi-pass membrane protein. Early endosome. Golgi apparatus, trans-Golgi network. Vacuole.
  • 蛋白家族：
    ZIP transporter (TC 2.A.5) family
  • 組織特異性：
    Expressed in the external cell layers of the root including the lateral branching zone. Also detected in flowers before pollination.
  • 數據庫鏈接：

    KEGG: ath:AT4G19690

    STRING: 3702.AT4G19690.2

    UniGene: At.2319



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