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  4. Recombinant Arabidopsis thaliana E3 ubiquitin-protein ligase SINAT2 (SINAT2)

Recombinant Arabidopsis thaliana E3 ubiquitin-protein ligase SINAT2 (SINAT2)

  • 中文名稱：
    擬南芥SINAT2重組蛋白
  • 貨號：
    CSB-YP864931DOA
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    擬南芥SINAT2重組蛋白
  • 貨號：
    CSB-EP864931DOA
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    擬南芥SINAT2重組蛋白
  • 貨號：
    CSB-EP864931DOA-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    擬南芥SINAT2重組蛋白
  • 貨號：
    CSB-BP864931DOA
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    擬南芥SINAT2重組蛋白
  • 貨號：
    CSB-MP864931DOA
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    SINAT2
  • Uniprot No.：
  • 別名：
    SINAT2; At3g58040; T10K17.250; E3 ubiquitin-protein ligase SINAT2; EC 2.3.2.27; RING-type E3 ubiquitin transferase SINAT2; Seven in absentia homolog 2
  • 種屬：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-308
  • 氨基酸序列
    MAPGGSALKE VMESNSTGMD YEVKTAKVEV NNNKPTKPGS AGIGKYGIHS NNGVYELLEC PVCTNLMYPP IHQCPNGHTL CSNCKLRVQN TCPTCRYELG NIRCLALEKV AESLEVPCRY QNLGCHDIFP YYSKLKHEQH CRFRPYTCPY AGSECSVTGD IPTLVVHLKD DHKVDMHDGC TFNHRYVKSN PHEVENATWM LTVFNCFGRQ FCLHFEAFQL GMAPVYMAFL RFMGDENEAK KFSYSLEVGA HGRKLTWQGI PRSIRDSHRK VRDSQDGLII PRNLALYFSG GDRQELKLRV TGRIWKEE
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. It probably triggers the ubiquitin-mediated degradation of different substrates. Mediates the proteasomal-dependent degradation of ATG6, a component of the autophagosome complex. Requires TRAF1A/MUSE14 and TRAF1B/MUSE13 to target ATG6 for ubiquitination and subsequent regulation of autophagosome assembly. Modulates directly the ubiquitination and proteasomal-dependent degradation of FREE1, a component of the ESCRT-I complex. Modulates directly the ubiquitination and proteasomal-dependent degradation of ELC/VPS23A, a component of the ESCRT-I complex.
  • 亞細胞定位：
    Endosome, multivesicular body. Cytoplasmic vesicle, autophagosome.
  • 蛋白家族：
    SINA (Seven in absentia) family
  • 數據庫鏈接：

    KEGG: ath:AT3G58040

    STRING: 3702.AT3G58040.1

    UniGene: At.34771



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