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  4. Recombinant Arabidopsis thaliana E3 ubiquitin-protein ligase PUB23 (PUB23)

Recombinant Arabidopsis thaliana E3 ubiquitin-protein ligase PUB23 (PUB23)

  • 中文名稱：
    擬南芥PUB23重組蛋白
  • 貨號：
    CSB-YP768015DOA
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    擬南芥PUB23重組蛋白
  • 貨號：
    CSB-EP768015DOA
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    擬南芥PUB23重組蛋白
  • 貨號：
    CSB-EP768015DOA-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    擬南芥PUB23重組蛋白
  • 貨號：
    CSB-BP768015DOA
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    擬南芥PUB23重組蛋白
  • 貨號：
    CSB-MP768015DOA
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    PUB23
  • Uniprot No.：
  • 別名：
    PUB23; At2g35930; F11F19.16; E3 ubiquitin-protein ligase PUB23; EC 2.3.2.27; Plant U-box protein 23; RING-type E3 ubiquitin transferase PUB23; U-box domain-containing protein 23
  • 種屬：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-411
  • 氨基酸序列
    MSGGIMDEEI EIPPFFLCPI SLEIMKDPVI VSTGITYDRD SIEKWLFAGK KNSCPVTKQD ITDADLTPNH TLRRLIQSWC TLNASYGVER IPTPRPPICK SEIEKLIRDS ASSHENQVKC LKRLRQIVSE NATNKRCLEA AGVPEFLANI VSNDSENGSL TDEALNLLYH LETSETVLKN LLNNKKDNNI VKSLTKIMQR GMYESRVYAT LLLKNILEVA DPMQSMTLKP EVFTEVVQIL DDRISQKATK AAMHILVNIC PWGRNRHKAV EAGVISVIIE LLMDESFTSE RRGPEMAMVV LDLLCQCAEG RAEFLNHGAA IAVVCKKILR VSQTASDRAV RVLLSVGRFC ATPALLHEML QLGVVAKLCL VLQVSCGGKT KEKAKELLKL HARVWKDSPC LPKNMILAYP C
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    E3 ubiquitin-protein ligase that negatively regulates water stress response. May control in coordination with PUB23 a drought signaling pathway by ubiquitinating cytosolic RPN12a. Acts as negative regulator of the immunity triggered by the pathogen-associated molecular patterns (PAMPs), in association with PUB22 and PUB24.
  • 基因功能參考文獻：
    1. RPN6 showed a shorter protein half-life in PUB22 overexpressing plants than in wild-type, and RPN6 was significantly stabilized in pub22pub23 double knockout plants. PMID: 26188517
    2. The results suggest that PUB23 coordinately control a drought signaling pathway by ubiquitinating cytosolic RPN12a in Arabidopsis. PMID: 18664614
    3. Negative regulation of PAMP-triggered immunity by an E3 ubiquitin ligase triplet (PUB22, PUB23, and PUB24) in Arabidopsis. [PUB23] PMID: 18771922
  • 亞細胞定位：
    Cytoplasm.
  • 數據庫鏈接：

    KEGG: ath:AT2G35930

    STRING: 3702.AT2G35930.1

    UniGene: At.43241



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