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  4. Recombinant Arabidopsis thaliana E3 SUMO-protein ligase MMS21 (MMS21)

Recombinant Arabidopsis thaliana E3 SUMO-protein ligase MMS21 (MMS21)

  • 中文名稱：
    擬南芥MMS21重組蛋白
  • 貨號：
    CSB-YP810169DOA
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    擬南芥MMS21重組蛋白
  • 貨號：
    CSB-EP810169DOA
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    擬南芥MMS21重組蛋白
  • 貨號：
    CSB-EP810169DOA-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    擬南芥MMS21重組蛋白
  • 貨號：
    CSB-BP810169DOA
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    擬南芥MMS21重組蛋白
  • 貨號：
    CSB-MP810169DOA
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    MMS21
  • Uniprot No.：
  • 別名：
    MMS21; HPY2; At3g15150; F4B12.6; E3 SUMO-protein ligase MMS21; EC 2.3.2.-; E3 SUMO-protein transferase MMS21; MMS21 homolog; AtMMS21; Protein HIGH PLOIDY 2
  • 種屬：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-249
  • 氨基酸序列
    MASASSSDGV AGRIQNASLV LVSDNSSTLA DIRKAVAMMK NIAVQLEKEN QTDKVKDLEN SVAELLDLHS DCNHRSTAIQ SVANRYQPVE QLTDFKKLLD DEFTKLKATP SSVPQNDHLM RQFREAVWNV HHAGEPMPGD DDEDIVMTST QCPLLNMTCP LSGKPVTELA DPVRSMDCRH VYEKSVILHY IVNNPNANCP VAGCRGKLQN SKVICDAMLK FEIEEMRSLN KQSNRAEVIE DFTEDVDED
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    E3 SUMO-protein ligase that modulates cell cycle progression and functions as a repressor of endocycle onset in meristems. May function downstream of the meristem patterning transcription factors PLETHORA 1 and 2 (PLT1 and PLT2) in root meristem development. Modulates the expression of the mitotic cyclins CYCB1-1 and CYCB1-2 and cyclin-dependent kinases CDKB1-1 and CDKB2-1 in root meristem. Involved in cytokinin signaling in root development.
  • 基因功能參考文獻：
    1. Mutation of AtMMS21 increased sensitivity to DNA damaging agents and the constitutive activation of DNA damage response in mms21-1 may be mediated by ATM. PMID: 24795278
    2. AtMMS21 mediated SUMOylation may stabilize the expression and accumulation of meiotic proteins and affect gametophyte development. PMID: 24893774
    3. These data reveal that AtMMS21 acts in DNA double-strand breaks amelioration and stem cell niche maintenance during Arabidopsis root development. PMID: 23426194
    4. conclude that MMS21 plays a role in the drought stress response, likely through regulation of gene expression in an ABA-dependent pathway PMID: 23231763
    5. SIZ1 and HPY2 function independently and that their combined SUMOylation is essential for plant development PMID: 23056518
    6. Arabidopsis SUMO E3 ligase AtMMS21 is involved in apical root meristem development. [MMS21] PMID: 20592809
    7. AtMMS21 is an important regulator of cell proliferation and cytokinin signalling during root development. PMID: 19682286

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  • 亞細胞定位：
    Nucleus. Cytoplasm.
  • 蛋白家族：
    NSE2 family
  • 數據庫鏈接：

    KEGG: ath:AT3G15150

    STRING: 3702.AT3G15150.1

    UniGene: At.39098



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