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  4. Recombinant Arabidopsis thaliana Copper-transporting ATPase PAA1, chloroplastic (PAA1), partial

Recombinant Arabidopsis thaliana Copper-transporting ATPase PAA1, chloroplastic (PAA1), partial

  • 中文名稱：
    擬南芥PAA1重組蛋白
  • 貨號：
    CSB-YP879890DOA
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    擬南芥PAA1重組蛋白
  • 貨號：
    CSB-EP879890DOA
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    擬南芥PAA1重組蛋白
  • 貨號：
    CSB-EP879890DOA-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    擬南芥PAA1重組蛋白
  • 貨號：
    CSB-BP879890DOA
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    擬南芥PAA1重組蛋白
  • 貨號：
    CSB-MP879890DOA
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    PAA1
  • Uniprot No.：
  • 別名：
    PAA1; GIR1; HMA6; At4g33520; F17M5.280; T16L1.10Copper-transporting ATPase PAA1; chloroplastic; EC 7.2.2.8; Protein HEAVY METAL ATPASE 6; Protein glucose insensitive root 1
  • 種屬：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Mediates copper transfer across the plastid envelope. Required for the delivery of copper into the plastid stroma, which is essential for the function of copper proteins. Seems to be selective for monovalent copper Cu(+) transport. Plays also a role in glucose signaling-mediated cell proliferation of root meristem in non-green tissues.
  • 基因功能參考文獻：
    1. The crystal structures of the nucleotide binding domain of HMA6 and HMA8 from Arabidopsis thaliana are reported at 1.5A and 1.75A resolution, respectively, providing the first structural information on plants Cu+-ATPases. PMID: 27802305
    2. Combining functional and structural data, we highlight the importance of the cysteine and the first histidine of the CX3HX2H motif in the process of copper release from HMA6 and HMA8 and propose a copper pathway through the membrane domain of these transporters. PMID: 27493208
    3. HMA6 enzymatic properties and affinity for Cu PMID: 26182363
    4. Plastid chaperone 1 (PCH1), which is produced as an alternative-splicing event of the PAA1 pre-mRNA, delivers Cu+ with specificity to PAA1. PMID: 25468978
    5. HMA1 and PAA1 behave as distinct pathways for copper import and targeting to the chloroplast. PMID: 24510941
    6. the plastidial Cu transporter PAA1, which is essential for plastid function and/or activity, plays an important role in bidirectional communication between the plastid and the nucleus in high glucose. PMID: 22582133
    7. the first enzymatic characterization of a P(IB-1)-type plant ATPase and clearly identify PAA1 as a high affinity Cu(I) transporter of the chloroplast envelope. PMID: 21878617

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  • 亞細胞定位：
    Plastid, chloroplast membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Cation transport ATPase (P-type) (TC 3.A.3) family, Type IB subfamily
  • 組織特異性：
    Expressed in the shoots and roots.
  • 數據庫鏈接：

    KEGG: ath:AT4G33520

    STRING: 3702.AT4G33520.2

    UniGene: At.48932



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