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  4. Recombinant Arabidopsis thaliana Chromo domain-containing protein LHP1 (LHP1)

Recombinant Arabidopsis thaliana Chromo domain-containing protein LHP1 (LHP1)

  • 中文名稱：
    擬南芥LHP1重組蛋白
  • 貨號：
    CSB-YP835992DOA
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    擬南芥LHP1重組蛋白
  • 貨號：
    CSB-EP835992DOA
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    擬南芥LHP1重組蛋白
  • 貨號：
    CSB-EP835992DOA-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    擬南芥LHP1重組蛋白
  • 貨號：
    CSB-BP835992DOA
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    擬南芥LHP1重組蛋白
  • 貨號：
    CSB-MP835992DOA
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    LHP1
  • Uniprot No.：
  • 別名：
    LHP1; TFL2; TU8; At5g17690; MVA3.4Chromo domain-containing protein LHP1; Protein LIKE HETEROCHROMATIN PROTEIN 1; Protein TERMINAL FLOWER 2
  • 種屬：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-445
  • 氨基酸序列
    MKGASGAVKK KPQVLNEAGE AETAVETVGE SRKISGDGGF GSDDGGGGGG GGSGESILRE IGDDRPTEDG DEEEEEDEDE DDGGDEEDEE GEGEGGQEER PKLDEGFYEI EAIRRKRVRK GKVQYLIKWR GWPETANTWE PLENLQSIAD VIDAFEGSLK PGKPGRKRKR KYAGPHSQMK KKQRLTSTSH DATEKSDSST SLNNSSLPDI PDPLDLSGSS LLNRDVEAKN AYVSNQVEAN SGSVGMARQV RLIDNEKEYD PTLNELRGPV NNSNGAGCSQ GGGIGSEGDN VRPNGLLKVY PKELDKNSRF IGAKRRKSGS VKRFKQDGST SNNHTAPTDQ NLTPDLTTLD SFGRIARMGN EYPGVMENCN LSQKTKIEEL DITKILKPMS FTASVSDNVQ EVLVTFLALR SDGKEALVDN RFLKAHNPHL LIEFYEQHLK YNRTP
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Structural component of heterochromatin involved in gene repression, including several floral homeotic genes and FLT that regulates flowering time. Required for maintenance of vernalization-induced repression of FLC. As part of the PRC1-like complex, recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me), leading to epigenetic repression. PcG PRC1 complex maintains the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility. Transcriptional repressor that binds DNA on GAGA-like motif and 5'-(A/G/T)AACCCTA(A/G)-3' consensus motif in the promoters of target genes. Recognizes and binds histone H3 tails methylated at 'Lys-27' (H3K27me) but depleted in active histone marks such as H3K4me, leading to epigenetic repression. When in complex with LHP1, recognizes and binds histone H3 tails methylated at 'Lys-4' (H3K4me) and 'Lys-27' (H3K27me), mostly corresponding to stress-responsive genes.
  • 基因功能參考文獻：
    1. a general role of LHP1 from local to higher conformation levels of chromatin configuration to determine its accessibility to define gene expression patterns PMID: 27410265
    2. Neither PRC2 activity nor LHP1 function are a major determinant for H2A monoubiquitination in Arabidopsis thaliana. PMID: 28403905
    3. Arabidopsis (Arabidopsis thaliana) GAGA-motif binding factor protein basic pentacysteine6 (BPC6) interacts with like heterochromatin protein1 (LHP1), a PRC1 component, and associates with vernalization2 (VRN2), a PRC2 component, in vivo. PMID: 26025051
    4. MSI1 serves to link PRC2 to LIKE HETEROCHROMATIN PROTEIN 1 (LHP1), a protein that binds H3K27me3 in vitro and in vivo and is required for a functional polycomb group system. PMID: 23778966
    5. TFL2 is required for normal seedling de-etiolation, greening and shade avoidance response through negative regulation of both PHYB-dependent R light signalling and PHYA-dependent FR light signalling. PMID: 22145973
    6. LIF2, involved in cell identity and cell fate decision, may modulate the activity of LHP1 at specific loci, during specific developmental windows or in response to environmental cues that control cell fate determination.[LIF2] PMID: 21304947
    7. In vivo, TFL2 is targeted to a number of the YUCCA genes in an auxin-dependent fashion revealing a role of TFL2 in auxin regulation, probably as a component of protein complexes affecting transcriptional control. PMID: 21251106
    8. We investigated the influence of heat stress on metabolites in the tu8 (a novel allele of TERMINAL FLOWER2) mutant. [TU8] PMID: 16025407
    9. In transgenic plants expressing the LHP1-GFP fusion protein, two major localization patterns were observed according to cell types suggesting that localization evolves with age or differentiation states. PMID: 16244868
    10. Results imply that LIKE HETEROCHROMATIN PROTEIN1 (LHP1) recruitment to chromatin is mediated through interaction with methyl K9 and that LHP1 controls different nuclear processes via transient binding to its nuclear sites. PMID: 16361394
    11. chromodomain protein LIKE HETEROCHROMATIN PROTEIN (LHP)1 might play a role in vernalization PMID: 16549797
    12. LIKE HETEROCHROMATIN PROTEIN 1 (LHP1) is necessary to maintain the epigenetically repressed state of FLC upon return to warm conditions typical of spring PMID: 16682972
    13. LHP1, like its animal homologues, has a high binding affinity for A/T-rich regions. PMID: 16972870
    14. Recognizes specifically trimethylated Histone H3 in vivo as part of a mechanism that represses the expression of many genes targeted by Polycomb repressive complex 2. PMID: 17542647
    15. Arabidopsis thaliana chromodomain-containing protein LHP1 colocalizes with H3K27me3 genome-wide. The LHP1 chromodomain also binds H3K27me3 with high affinity, suggesting that LHP1 has functions similar to those of Polycomb PMID: 17676062
    16. disruption of the chromodomain of LHP1 abolishes H3K27me3 recognition, releases gene silencing and causes similar phenotypic alterations as transcriptional lhp1 null mutants PMID: 19399177

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  • 亞細胞定位：
    Nucleus. Note=Punctuated distribution and in heterochromatic chromocenters.
  • 組織特異性：
    Mainly expressed in meristematic tissues of vegetative, inflorescence and floral organs, like in developing ovules, petals, root pericycle, petiole and petiole side of developing leaf blade or vascular tissue.
  • 數據庫鏈接：

    KEGG: ath:AT5G17690

    STRING: 3702.AT5G17690.1

    UniGene: At.7322



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