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  4. Recombinant Agrobacterium tumefaciens Single-strand DNA-binding protein (virE2), partial

Recombinant Agrobacterium tumefaciens Single-strand DNA-binding protein (virE2), partial

  • 中文名稱：
    根癌土壤桿菌virE2重組蛋白
  • 貨號：
    CSB-YP363461AEZ
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    根癌土壤桿菌virE2重組蛋白
  • 貨號：
    CSB-EP363461AEZ-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    根癌土壤桿菌virE2重組蛋白
  • 貨號：
    CSB-BP363461AEZ
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    根癌土壤桿菌virE2重組蛋白
  • 貨號：
    CSB-MP363461AEZ
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    virE2
  • Uniprot No.：
  • 別名：
    virE2; Single-strand DNA-binding protein
  • 種屬：
    Agrobacterium tumefaciens (strain 15955)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Involved in DNA transformation; mediates the nuclear uptake of single-stranded DNA copies of the transferred DNA (T-DNA) element. Binds single-stranded but not double-stranded DNA regardless of nucleotide sequence composition.
  • 基因功能參考文獻：
    1. Both the VirE2 cargo motifs and AP2M were important for the transformation process. Because AP2-mediated endocytosis is well conserved, our data suggest that the A. tumefaciens pathogen hijacks conserved endocytic pathways to facilitate the delivery of virulence factors. PMID: 28345032
    2. A tetracysteine mutant (VirE2-TC) was prepared for in vitro and in vivo fluorescence imaging. VirE2-TC was found to be biochemically active as it binds both ssDNA and the acidic secretion chaperone VirE1. It was also biologically functional in complementing virE2 null strains transforming Arabidopsis thaliana roots and Nicotiana tabacum leaves. PMID: 28403156
    3. Data shos theat the virulence gene virE2 expression from cumic acid-induced promoters resulted in 47 to 72% of wild-type T-DNA transfer. PMID: 26292850
    4. The authors of the study propose a model for interaction of VirE2 with DNA. PMID: 26044711
    5. upon interaction with ssDNA recombinant protein, VirE2 is able to alter conformation of the latter by shortening the initial length of the ssDNA. PMID: 22117554
    6. Osa blocks stable transfer of RSF1010 to plant cells by inhibiting transfer of VirE2, which is essential for the transfer of RSF1010 into plant cells. PMID: 15489437
    7. Agrobacterium protein links with histoness in plant chromatin. PMID: 16010006
    8. Cooperative binding of VirE2 to single-stranded DNA releases VirE1, resulting in a controlled formation mechanism for the helical complex that is further promoted by macromolecular crowding. PMID: 17060320

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  • 亞細胞定位：
    Secreted. Host nucleus.


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