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  4. Recombinant Adenosine monophosphate-protein transferase vopS (vopS)

Recombinant Adenosine monophosphate-protein transferase vopS (vopS)

  • 中文名稱：
    副溶血性弧菌vopS重組蛋白
  • 貨號：
    CSB-YP774986VFE
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    副溶血性弧菌vopS重組蛋白
  • 貨號：
    CSB-EP774986VFE
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    副溶血性弧菌vopS重組蛋白
  • 貨號：
    CSB-EP774986VFE-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    副溶血性弧菌vopS重組蛋白
  • 貨號：
    CSB-BP774986VFE
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    副溶血性弧菌vopS重組蛋白
  • 貨號：
    CSB-MP774986VFE
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    vopS
  • Uniprot No.：
  • 別名：
    vopS; VP1686Protein adenylyltransferase VopS; EC 2.7.7.n1; AMPylator VopS; Vibrio outer protein S
  • 種屬：
    Vibrio parahaemolyticus
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-387
  • 氨基酸序列
    MISFGNVSAL QAAMPQARNE ILNEGKLSIG GKEYTINAAT QEFTRANPTS GAVARFFEAT GKLFREGSTQ SVAKAITKAV FDNEQGQAQR LQTSSSVEHG QMLFKDANLK TPSDVLNAFA KLDSKMVKSH AAELSQLAER AMTEVMLETD SGKNLKALIG DDAVKSLAVR VVKDYGGGVA AAQKNPEVRI NQMQAVFDME VMHLKAAQRH IEGLASTDLN QGVYAEGLPE DAFNKAGVTN NVERAAAWII NASNSKGNDA ENITSLLKEY ATNGKDLLNM DNLKELHARL VPNVERDYRG PNISGGTLPS SIGGEGMLKQ HIEGFLKENP VADKDLGKHL FAGVIGYHGF TDGNGRMGRM LYAIAELRND SFNPLAMNAE NSLHGIK
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Adenylyltransferase involved in virulence by mediating the addition of adenosine 5'-monophosphate (AMP) to specific threonine residue of host Rho GTPases RhoA, Rac and Cdc42. The resulting AMPylation prevents the interaction of Rho GTPases with downstream effectors, thereby inhibiting actin assembly in infected cells.
  • 基因功能參考文獻：
    1. appears to be one of 3 TTSS effector proteins in V. parahaemolyticus PMID: 16428750
    2. Vibrio effector protein VP1686 activates a conserved apoptotic pathway in macrophages through suppression of NF-kappaB activation independent of Toll-like receptor signaling PMID: 16984916
    3. We report that Vibrio parahaemolyticus induces a rapid remodeling of macrophage actin and activates RhoB GTPase via type III secretion system 1 regulated translocation of the effector protein, VP1686, into the macrophages. PMID: 18239436
    4. The VopS effector (VP1686) fic domain AMPylates Rho-family GTPases to disrupt effector binding and downstream signaling. PMID: 19039103
    5. Required for Rho-family GTPase inhibition PMID: 18347050

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  • 亞細胞定位：
    Secreted. Note=Translocated into the host cell via the type III secretion system (TTSS).
  • 數據庫鏈接：

    KEGG: vpa:VP1686

    STRING: 223926.VP1686



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