在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

  1. Home
  2. 抗體
  3. 重組抗體
  4. ULK3 Recombinant Monoclonal Antibody

ULK3 Recombinant Monoclonal Antibody

  • 中文名稱：
    ULK3 Recombinant Monoclonal Antibody
  • 貨號：
    CSB-RA969203A0HU
  • 規格：
    ￥1320
  • 圖片：
    • IHC image of CSB-RA969203A0HU diluted at 1:100 and staining in paraffin-embedded human colorectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • IHC image of CSB-RA969203A0HU diluted at 1:100 and staining in paraffin-embedded human stomach tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • Immunofluorescence staining of Hela cell with CSB-RA969203A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Overlay Peak curve showing PC-3 cells stained with CSB-RA969203A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for?10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他：

產品詳情

  • Uniprot No.：
  • 基因名：
    ULK3
  • 別名：
    Serine/threonine-protein kinase ULK3 (EC 2.7.11.1) (Unc-51-like kinase 3), ULK3
  • 反應種屬：
    Human
  • 免疫原：
    A synthesized peptide from human ULK3 protein
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    4D2
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 產品提供形式：
    Liquid
  • 應用范圍：
    ELISA, IHC, IF, FC
  • 推薦稀釋比：
    Application Recommended Dilution
    IHC 1:50-1:200
    IF 1:50-1:200
    FC 1:50-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能：
    Serine/threonine protein kinase that acts as a regulator of Sonic hedgehog (SHH) signaling and autophagy. Acts as a negative regulator of SHH signaling in the absence of SHH ligand: interacts with SUFU, thereby inactivating the protein kinase activity and preventing phosphorylation of GLI proteins (GLI1, GLI2 and/or GLI3). Positively regulates SHH signaling in the presence of SHH: dissociates from SUFU, autophosphorylates and mediates phosphorylation of GLI2, activating it and promoting its nuclear translocation. Phosphorylates in vitro GLI2, as well as GLI1 and GLI3, although less efficiently. Also acts as a regulator of autophagy: following cellular senescence, able to induce autophagy.
  • 基因功能參考文獻：
    1. Increased ULK3 also induces autophagy, which is unlinked from GLI and CAF activation. ULK3 upregulation occurs in the CAFs of several tumor types, and ULK3 silencing suppresses the tumor-enhancing properties of these cells. PMID: 28877478
    2. Structural and biochemical studies reveal an unusually tight interaction between ULK3 and IST1, an ESCRT-III subunit required for cytokinetic abscission. PMID: 26011858
    3. First named and identified as protein kinase of the ULK family PMID: 12471243
    4. Dual function of UNC-51-like kinase 3 (Ulk3) in the Sonic hedgehog signaling pathway. PMID: 20643644
    5. serine/threonine kinase ULK3 is involved in the SHH pathway as a positive regulator of GLI proteins PMID: 19878745

    顯示更多

    收起更多

  • 亞細胞定位：
    Cytoplasm. Note=Localizes to pre-autophagosomal structure during cellular senescence.
  • 蛋白家族：
    Protein kinase superfamily, Ser/Thr protein kinase family, APG1/unc-51/ULK1 subfamily
  • 組織特異性：
    Widely expressed. Highest levels observed in fetal brain. In adult tissues, high levels in brain, liver and kidney, moderate levels in testis and adrenal gland and low levels in heart, lung, stomach, thymus, prostate and placenta. In the brain, highest ex
  • 數據庫鏈接：

    HGNC: 19703

    OMIM: 613472

    KEGG: hsa:25989

    STRING: 9606.ENSP00000400312

    UniGene: Hs.513034



主站蜘蛛池模板： 国模大尺度福利视频在线| 久精品国产欧美亚洲色aⅴ大片| 中文字幕人妻熟女人妻| 精品国产亚洲一区二区三区 | 色资源av中文无码先锋| 亚洲国产精品无码久久久动漫| 好爽好湿好硬好大免费视频 | 国产精品熟妇一区二区三区四区| 狂野欧美性猛交免费视频| 亚洲天堂2017无码中文| 99精品国产福利在线观看| 国产在线精品欧美日韩电影| 国产成人无码av一区二区| 国产极品视觉盛宴| 国产毛多水多高潮高清| 仙踪林果冻传媒一区二区| 久久综合久久自在自线精品自| 18禁男女无遮挡啪啪网站| 屁屁影院ccyy备用地址| 亚洲欧美丝袜精品久久| 老熟女毛茸茸浓毛| 国产在线无码一区二区三区| 亚洲综合精品香蕉久久网| 国产精品自在拍在线拍| 精品人妻大屁股白浆无码| 无码av免费一区二区三区a片| 人人澡人人澡人人看添| 国产在线视频一区二区三区98 | 国产亚洲精品电影网站在线观看 | 水蜜桃av无码| 中日韩精品无码一区二区三区| 午夜嘿嘿嘿影院| 国产爆乳美女娇喘呻吟| 久久免费精品国产72精品| 亚洲国产老鸭窝一区二区三区| 成人美女黄网站色大免费的| 免费观看羞羞视频网站| 亚洲综合另类小说色区| 无码专区6080yy国产电影| 久久不见久久见免费视频7| 性生交大片免费看l|