在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

  1. Home
  2. 抗體
  3. 重組抗體
  4. RANBP3 Recombinant Monoclonal Antibody

RANBP3 Recombinant Monoclonal Antibody

  • 中文名稱：
    RANBP3 Recombinant Monoclonal Antibody
  • 貨號：
    CSB-RA831539A0HU
  • 規格：
    ￥1320
  • 圖片：
    • IHC image of CSB-RA831539A0HU diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • Immunofluorescence staining of A-431 cell with CSB-RA831539A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Overlay Peak curve showing HepG2 cells stained with CSB-RA831539A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for?10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他：

產品詳情

  • Uniprot No.：
  • 基因名：
    RANBP3
  • 別名：
    DKFZp586I1520 antibody; Ran binding protein 3 antibody; Ran-binding protein 3 antibody; RANB3_HUMAN antibody; RanBP3 antibody
  • 反應種屬：
    Human
  • 免疫原：
    A synthesized peptide derived from human RANBP3
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    4B3
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
  • 產品提供形式：
    Liquid
  • 應用范圍：
    ELISA, IHC, IF, FC
  • 推薦稀釋比：
    Application Recommended Dilution
    IHC 1:50-1:200
    IF 1:50-1:200
    FC 1:50-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能：
    Acts as a cofactor for XPO1/CRM1-mediated nuclear export, perhaps as export complex scaffolding protein. Bound to XPO1/CRM1, stabilizes the XPO1/CRM1-cargo interaction. In the absence of Ran-bound GTP prevents binding of XPO1/CRM1 to the nuclear pore complex. Binds to CHC1/RCC1 and increases the guanine nucleotide exchange activity of CHC1/RCC1. Recruits XPO1/CRM1 to CHC1/RCC1 in a Ran-dependent manner. Negative regulator of TGF-beta signaling through interaction with the R-SMAD proteins, SMAD2 and SMAD3, and mediating their nuclear export.
  • 基因功能參考文獻：
    1. Computational docking simulation indicates that phosphorylation of RanBP3 at Ser58 could potentially stabilize the association of RanBP3 with importin-alpha through interactions between the phosphate moiety of phospho-Ser58 of RanBP3 and a cluster of basic residues (Arg96 and Lys97 in importin-alpha3) on armadillo repeat 1 of importin-alpha. PMID: 28760339
    2. RANBP3 regulates melanoma cell proliferation and probably a potential target for molecular targeted therapy. PMID: 26763446
    3. RanBP3 is involved in vRNP nuclear export. The function of RanBP3 during vRNP nuclear export is regulated by phosphorylation at Ser58. RanBP3 phosphorylation is modulated by both PI3K/Akt and Ras/ERK/RSK pathways in the late phase of viral infection. PMID: 23303829
    4. Crystal structure of the Ran-binding domain (RBD) from RanBP3 and compare it to RBD structures from RanBP1 and RanBP2 in complex with Ran and CRM1. PMID: 21364925
    5. RanBP3 stimulates export by enhancing the affinity of Crm1 for Ran-GTP and cargo. PMID: 11932251
    6. A multifunctional domain in CRM1 (exportin 1) mediates RanBP3 binding and multimerization of human T-cell leukemia virus type 1 Rex protein. PMID: 14612415
    7. RANBP3 phosphorylation links the Ras and PI3-kinase pathways to nucleocytoplasmic transport. PMID: 18280241
    8. RanBP3 directly recognizes dephosphorylated Smad2/3, which results from the activity of nuclear Smad phosphatases, and mediates nuclear export of Smad2/3 in a Ran-dependent manner. PMID: 19289081

    顯示更多

    收起更多

  • 亞細胞定位：
    Cytoplasm. Nucleus.
  • 組織特異性：
    Widely expressed with high levels in testis and heart.
  • 數據庫鏈接：

    HGNC: 9850

    OMIM: 603327

    KEGG: hsa:8498

    STRING: 9606.ENSP00000341483

    UniGene: Hs.531752



主站蜘蛛池模板： 四虎影视在线影院在线观看免费视频 | 亚洲欧美在线一区中文字幕| 男人猛躁进女人免费播放| 天天爽夜夜爱| 亚洲的天堂av无码| 成人h动漫无码网站久久| 丝袜高潮流白浆潮喷在线播放| 午夜人妻久久久久久久久| 中文字幕av在线一二三区| 久久99青青精品免费观看 | 99re在线播放| 久久青草费线频观看| 黄在线看片免费人成视频| 性欧美videofree高清精品| 人妻av无码一区二区三区| 国产熟妇高潮叫床视频播放| 中文字幕人妻熟女av| h漫全彩纯肉无码网站| 精品久久无码中文字幕| 极品少妇hdxx麻豆hdxx| 国内无遮挡18禁无码网站免费| 精品无码国模私拍视频| 国产女人好紧好爽| 欧美日韩人人模人人爽人人喊| 亚洲欧洲日产国产 最新| 色吊丝中文字幕| 国产成人精品a视频一区| 极品无码av国模在线观看| 色八区人妻在线视频免费| 麻豆网神马久久人鬼片| 做爰高潮视频免费的看| 美女扒开腿让男人桶爽app免费看 精品国产免费人成电影在线看 | 国产老熟女狂叫对白| 中文字幕乱码人妻无码久久| 成人精品一区日本无码网站| 国产无遮挡免费真人视频在线观看| 精品无码国产污污污免费| 免费的很黄很污的视频| 99国产成人精品视频| 久久久久久久久888| 久久久精品国产sm最大网站|