在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

  1. Home
  2. 抗體
  3. 重組抗體
  4. PSMA3 Recombinant Monoclonal Antibody

PSMA3 Recombinant Monoclonal Antibody

  • 中文名稱：
    PSMA3 Recombinant Monoclonal Antibody
  • 貨號：
    CSB-RA140307A0HU
  • 規格：
    ￥1320
  • 圖片：
    • Western Blot
      Positive WB detected in: HT-29 whole cell lysate(30μg), A549 whole cell lysate(30μg), MCF-7 whole cell lysate(30μg), Hela whole cell lysate(30μg), PC-3 whole cell lysate(30μg), K562 whole cell lysate(30μg), HL-60 whole cell lysate(30μg), NIH/3T3 whole cell lysate(30μg), Raw264.7 whole cell lysate(30μg)
      All lanes: PSMA3 antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/40000 dilution
      Predicted band size: 28 kDa
      Observed band size: 28 kDa
      Exposure time：1min20s
    • IHC image of CSB-RA140307A0HU diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • IHC image of CSB-RA140307A0HU diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • Overlay Peak curve showing BxPC-3 cells stained with CSB-RA140307A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for?10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他：

產品詳情

  • Uniprot No.：
  • 基因名：
    PSMA3
  • 別名：
    Proteasome subunit alpha type-3 (EC 3.4.25.1) (Macropain subunit C8) (Multicatalytic endopeptidase complex subunit C8) (Proteasome component C8), PSMA3, HC8 PSC8
  • 反應種屬：
    Human, Mouse
  • 免疫原：
    A synthesized peptide from human PSMA3 protein
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    5A8
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 產品提供形式：
    Liquid
  • 應用范圍：
    ELISA, WB, IHC, FC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:2000
    IHC 1:50-1:200
    FC 1:50-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能：
    Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex). Binds to the C-terminus of CDKN1A and thereby mediates its degradation. Negatively regulates the membrane trafficking of the cell-surface thromboxane A2 receptor (TBXA2R) isoform 2.
  • 基因功能參考文獻：
    1. High PSMA3 expression is associated with cholangiocarcinoma. PMID: 28560424
    2. Our results provide evidence on new T1DM-susceptible loci in the PSMA3, PSMA6 and PSMC6 proteasome genes and give a new insight into the T1DM pathogenesis PMID: 26661414
    3. Evidence of a sex-specific association of PSMA3 genetic variants with subtypes of juvenile idiopathic arthritis. PMID: 24875235
    4. Protein interaction between Ambn and Psma3 can facilitate redistribution of ameloblastin domains within forming enamel. PMID: 26070558
    5. A combination of two-dimensional gel electrophoresis (2D-GE) and tandem mass-spectrometry revealed a large number of PSMA3-bound proteins that are involved in various aspects of mRNA metabolism, including splicing. PMID: 22079093
    6. Serum 20S proteasome concentration and percentage of lymphocytic apoptosis predicted survival and patient prognosis in critically ill patients. PMID: 21108816
    7. Plasminogen activator inhibitor type 1 interacts with alpha3 subunit of proteasome and modulates its activity. PMID: 21135093
    8. Aurora-B might undergo degradation by binding to HC8 in a proteasome-dependent manner during mitosis PMID: 14674694
    9. EBNA3C binds to human the C8/alpha7 subunit of the 20S proteasome; degraded by purified 20S proteasomes in vitro but, found to be very stable and apparently turned over at a very low rate in B cells infected latently with EBV PMID: 15831937
    10. The authors propose that the full-length HCV F protein as well as the F protein initiating from codon 26 is degraded by an ubiquitin-independent pathway that is mediated by the proteasome subunit alpha3. PMID: 18971267

    顯示更多

    收起更多

  • 亞細胞定位：
    Cytoplasm. Nucleus.
  • 蛋白家族：
    Peptidase T1A family
  • 數據庫鏈接：

    HGNC: 9532

    OMIM: 176843

    KEGG: hsa:5684

    STRING: 9606.ENSP00000216455

    UniGene: Hs.558799



主站蜘蛛池模板： 精品久久久久久中文字幕| 亚洲成av人在线观看网站| 无码丰满少妇2在线观看| 国产爆乳美女娇喘呻吟| 国内高清久久久久久| 亚洲欧美日韩国产另类电影| 果冻传媒亚洲区二期| 欧美日韩精品一区二区在线播放 | 精品人妻系列无码一区二区三区 | 国产精品无码免费专区午夜| 无码国产精成人午夜视频一区二区| 拍国产乱人伦偷精品视频| 天天av天天av天天透| 夜夜高潮天天爽欧美| 无尺码精品产品日韩| 日韩精品无码免费一区二区三区| 久久99精品久久久久久蜜芽 | 精产国品一二三产品麻豆| 少妇人妻偷人精品视频| 亚洲乱码精品久久久久.. | 国产无遮挡a片又黄又爽| 日本精品中文字幕在线播放| 在线观看片免费人成视频无码| 99精品国产成人一区二区| 国产免费一区二区三区免费视频| 亚洲a∨精品永久无码| 久久亚洲国产精品五月天婷| 国精产品一二三区传媒公司 | 亚洲国产精品第一区二区三区| 18禁超污无遮挡无码免费动态图| 欧美xxxxx高潮喷水| 麻豆av传媒蜜桃天美传媒| 日本一上一下爱爱免费| 图片区 小说区 区 亚洲五月 | 亚洲日韩精品一区二区三区无码| 天天av天天av天天透| 天天躁日日躁狠狠躁视频2021| 性一交一乱一色一视频| 日日碰日日摸夜夜爽无码| 激情欧美成人久久综合| 装睡被陌生人摸出水好爽|