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  4. PIN4 Recombinant Monoclonal Antibody

PIN4 Recombinant Monoclonal Antibody

  • 中文名稱：
    PIN4重組抗體
  • 貨號(hào)：
    CSB-RA916699A0HU
  • 規(guī)格：
    ￥1320
  • 圖片：
    • Western Blot
      Positive WB detected in: U251 whole cell lysate, MCF-7 whole cell lysate, HEK293 whole cell lysate, Hela whole cell lysate, Jurkat whole cell lysate, PC3 whole cell lysate
      All lanes: PIN4 antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 14, 17, 15 kDa
      Observed band size: 12-17 kDa
    • IHC image of CSB-RA916699A0HU diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • Immunofluorescence staining of MCF-7 cell with CSB-RA916699A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 598-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    CSB-RA916699A0HU重組單克隆抗體是針對(duì)PIN4蛋白研發(fā)的高特異性試劑，適用于多種蛋白檢測(cè)場(chǎng)景。PIN4（Peptidyl-prolyl cis/trans isomerase）屬于肽酰脯氨酰順?lè)串悩?gòu)酶家族，通過(guò)調(diào)控磷酸化蛋白的構(gòu)象變化參與細(xì)胞周期調(diào)控、應(yīng)激響應(yīng)及腫瘤發(fā)生等過(guò)程，在癌癥與神經(jīng)退行性疾病研究中備受關(guān)注。本抗體經(jīng)ELISA、Western Blot（WB）、免疫組化（IHC）和免疫熒光（IF）多平臺(tái)驗(yàn)證，在WB實(shí)驗(yàn)中可清晰識(shí)別天然蛋白，IHC顯示組織定位特異性，IF檢測(cè)呈現(xiàn)亞細(xì)胞水平的高分辨率信號(hào)，推薦稀釋度分別為WB 1:500-1:2000、IHC 1:50-1:200、IF 1:50-1:200。其低交叉反應(yīng)性和高批次穩(wěn)定性使其成為研究PIN4在細(xì)胞周期異常、蛋白折疊障礙及相關(guān)疾病分子機(jī)制中的理想工具，適用于腫瘤生物學(xué)、神經(jīng)病理學(xué)及基礎(chǔ)蛋白質(zhì)相互作用研究領(lǐng)域。
  • Uniprot No.：
  • 基因名：
    PIN4
  • 別名：
    Peptidyl-prolyl cis-trans isomerase NIMA-interacting 4 (EC 5.2.1.8) (Parvulin-14) (Par14) (hPar14) (Parvulin-17) (Par17) (hPar17) (Peptidyl-prolyl cis-trans isomerase Pin4) (PPIase Pin4) (Peptidyl-prolyl cis/trans isomerase EPVH) (hEPVH) (Rotamase Pin4), PIN4
  • 反應(yīng)種屬：
    Human
  • 免疫原：
    A synthesized peptide derived from human PIN4
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標(biāo)記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號(hào)：
    14A7
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, WB, IHC, IF
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:2000
    IHC 1:50-1:200
    IF 1:50-1:200
  • Protocols：
  • 儲(chǔ)存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Isoform 1 is involved as a ribosomal RNA processing factor in ribosome biogenesis. Binds to tightly bent AT-rich stretches of double-stranded DNA.; Isoform 2 binds to double-stranded DNA.
  • 基因功能參考文獻(xiàn)：
    1. Par14 can be described as an endogenous non-histone chromatin protein, which binds DNA in vivo PMID: 25645591
    2. Ca(2+)/Calmodulin binding to the N-terminal of Par17 causes steric hindrance of the Par17 active site, thus interfering with the Par17/tubulin interaction. PMID: 25940090
    3. Data indicate that Pin1 prolyl isomerase active site cysteine, Cys113, is highly susceptible to oxidation. PMID: 25595659
    4. Data indicate the association of Par14 with insulin receptor substrate 1 (IRS-1) in human HepG2 cells overexpressing both as well as endogenously in the mouse liver. PMID: 23720771
    5. The N-terminal basic domain of human parvulin hPar14 is responsible for the entry to the nucleus and high-affinity DNA-binding. PMID: 12144781
    6. subcellular localization and DNA binding properties of hPar14 are regulated by phosphorylation of the N-terminal domain PMID: 12860119
    7. Identification of a longer Parvulin isoform (Par17) that has an extension at the 5' end including a 75 bp extended open reading frame. PMID: 16522211

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  • 亞細(xì)胞定位：
    [Isoform 1]: Nucleus, nucleolus. Cytoplasm, cytoskeleton, spindle. Cytoplasm. Note=Colocalizes in the nucleolus during interphase and on the spindle apparatus during mitosis with NPM1.; [Isoform 2]: Mitochondrion. Mitochondrion matrix. Note=Imported in a time- and membrane potential-dependent manner to the mitochondrial matrix, but without concomitant processing of the protein. Directed to mitochondria by a novel N-terminal domain that functions as non-cleavable mitochondrial targeting peptide.
  • 蛋白家族：
    PpiC/parvulin rotamase family, PIN4 subfamily
  • 組織特異性：
    Isoform 2 is much more stable than isoform 1 (at protein level). Ubiquitous. Isoform 1 and isoform 2 are expressed in kidney, liver, blood vessel, brain, mammary gland, skeletal muscle, small intestine and submandibularis. Isoform 1 transcripts are much m
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 8992

    OMIM: 300252

    KEGG: hsa:5303

    STRING: 9606.ENSP00000362773

    UniGene: Hs.655623



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