在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

  1. Home
  2. 抗體
  3. 重組抗體
  4. NTHL1 Recombinant Monoclonal Antibody

NTHL1 Recombinant Monoclonal Antibody

  • 中文名稱：
    NTHL1重組抗體
  • 貨號：
    CSB-RA832492A0HU
  • 規格：
    ￥1320
  • 圖片：
    • Western Blot
      Positive WB detected in: HepG2 whole cell lysate, HEK293 whole cell lysate, PC-3 whole cell lysate
      All lanes: NTHL1 antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 35, 34, 33 kDa
      Observed band size: 35 kDa
    • IHC image of CSB-RA832492A0HU diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • Immunofluorescence staining of PC-3 cell with CSB-RA832492A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 604-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Overlay Peak curve showing HepG2 cells stained with CSB-RA832492A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他：

產品詳情

  • 產品描述：
    CSB-RA832492A0HU NTHL1重組單克隆抗體是針對DNA損傷修復關鍵酶NTHL1研發的高特異性科研工具。該抗體通過重組技術制備，具有嚴格的批次穩定性和高親和力，可精準識別人類來源的NTHL1蛋白。經多種實驗驗證，在Western Blot中能清晰檢測目標蛋白表達，免疫組化實驗顯示其在組織切片中的特異性定位，免疫熒光和流式細胞術則證實其適用于亞細胞水平及細胞表面標記分析。推薦使用稀釋度覆蓋WB 1:500-1:2000、IHC 1:50-1:200、IF/FC 1:50-1:200的寬泛工作濃度。該產品適用于探索NTHL1在DNA堿基切除修復、氧化應激響應中的分子機制，尤其在結直腸癌、乳腺癌等腫瘤基因組不穩定性研究中具有重要價值，可配合細胞損傷模型、腫瘤微環境分析等實驗體系，為基因修復通路研究和癌癥相關生物標志物發現提供可靠檢測工具。
  • Uniprot No.：
  • 基因名：
  • 別名：
    Endonuclease III-like protein 1 (hNTH1) (EC 3.2.2.-) (EC 4.2.99.18) (Bifunctional DNA N-glycosylase/DNA-(apurinic or apyrimidinic site) lyase) (DNA glycosylase/AP lyase), NTHL1, NTH1 OCTS3
  • 反應種屬：
    Human
  • 免疫原：
    A synthesized peptide derived from human NTHL1
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    22H10
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
  • 產品提供形式：
    Liquid
  • 應用范圍：
    ELISA, WB, IHC, IF, FC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:2000
    IHC 1:50-1:200
    IF 1:50-1:200
    FC 1:50-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能：
    Bifunctional DNA N-glycosylase with associated apurinic/apyrimidinic (AP) lyase function that catalyzes the first step in base excision repair (BER), the primary repair pathway for the repair of oxidative DNA damage. The DNA N-glycosylase activity releases the damaged DNA base from DNA by cleaving the N-glycosidic bond, leaving an AP site. The AP-lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination. Primarily recognizes and repairs oxidative base damage of pyrimidines. Has also 8-oxo-7,8-dihydroguanine (8-oxoG) DNA glycosylase activity. Acts preferentially on DNA damage opposite guanine residues in DNA. Is able to process lesions in nucleosomes without requiring or inducing nucleosome disruption.
  • 基因功能參考文獻：
    1. The processing of the lesions was evaluated by purified enzyme cocktails of hNTH1 and hOGG1 as well as with a HeLa cell extract. Interestingly, the yield of double-strand breaks (DSBs) resulting from the processing of the bistranded lesions are appreciably lower when the DNA is treated with the HeLa cell extract compared with the relevant purified enzyme cocktail in both models PMID: 30207305
    2. NTHL1 in these extracts was able to excise thymine glycol from both naked DNA and sites in nucleosomes that earlier studies had shown to be sterically accessible. However, the same extracts were able to excise lesions from sterically-occluded sites in nucleosomes only after the addition of Mg2+/ATP. PMID: 28709015
    3. WT NTHL1 (human) and Nth (E. coli) are remarkably alike with respect to specificity of glycosylase reaction, and although NTHL1 is a much slower enzyme than Nth, the tighter binding of NTHL1 compensates, resulting in similar kcat/Kd values for both enzymes with each of the substrates tested. For NTHL1 Gln287Ala, specificity for substrates positioned opposite G is lost, but not that of substrates positioned opposite A. PMID: 28292631
    4. We therefore found published evidence to support the association between variants in NTHL1 and RPS20 with CRC. PMID: 27713038
    5. Both Ntg1 and its human homologue, NTHL1, can be SUMO-modified in response to oxidative stress. PMID: 27839712
    6. NTH1 polymorphisms may be associated with non-small cell lung cancer pathogenesis. PMID: 26400813
    7. This study extends the description of biallelic mutations in NTHL1 beyond the single c.268C-->T,p.Q90* mutation that was observed previously. PMID: 26559593
    8. A homozygous loss-of-function germline mutation in the NTHL1 gene predisposes to a new subtype of BER-associated adenomatous polyposis and colorectal cancer. PMID: 25938944
    9. we demonstrated that hNEIL1 and hNTH1 cleave Oz sites as efficiently as 5-hydroxyuracil sites. Thus, hNEIL1 and hNTH1 can repair Oz lesions PMID: 22465744
    10. NTH1 is involved in removal of 8-oxoguanine from 8-oxoguanine/guanine mispairs in DNA PMID: 11328882
    11. recombinant hNTH1 lacking 55, 72 or 80 residues from the N terminus had four- to fivefold higher activities than the full-length enzyme PMID: 12144783
    12. substrate selectivity of mammalian NTH1 and the concomitant selective stimulation of activity by APE1 are indicative of selective repair of oxidative damage in different regions of the genome PMID: 12519758
    13. dimerization of hNTH1 involving the N-terminal tail masks the inhibitory effect of this tail and plays a critical role in its catalytic turnover in the cell PMID: 14522981
    14. hNTH1 and hNEIL1 but not hNEIL2 excised the two stereoisomers of thymine glycol (5R-Tg and 5S-Tg), but their isomer specificity was markedly different PMID: 14734554
    15. search for the factors interacting with NTH1 shows GST-NTH1 fusion protein precipitates proliferating cell nuclear antigen (PCNA) and p53 as well as XPG from human cell-free extracts PMID: 15358233
    16. NTH1 is a DNA glycosylase that excise 5-formyluracil, 5-hydroxymethyluracil and Thymine glycol in human cells. PMID: 15533839
    17. hNTH1 plays two roles in the processing of radiation damages: repair of potentially lethal single lesions and generation of lethal double strand breaks at clustered damage sites. PMID: 16111924
    18. Nth1 released 5R,6S 2'-deoxyribonucleoside diastereoisomer (Tg2) much more rapidly than cis 5S,6R-deoxyribonucleoside diastereoisomer (Tg1) regardless of the opposing purine. Neil1 released Thymine glycol non-stereoselectively. PMID: 16446124
    19. The damage specificity of human homologues of Endo III (hNTHl) and Endo VIII (hNEIL1 and hNEIL2) is compared to elucidate the repair role in cells. PMID: 17150535
    20. These observations suggest that access to sterically occluded lesions entails the partial, reversible unwrapping of DNA from the histone octamer, allowing hNTH1 to capture its DNA substrate when it is in an unwound state. PMID: 17923696
    21. Downregulation of NTH1 is associated with gastric cancer. PMID: 19414504

    顯示更多

    收起更多

  • 相關疾病：
    Familial adenomatous polyposis 3 (FAP3)
  • 亞細胞定位：
    Nucleus. Mitochondrion.
  • 蛋白家族：
    Nth/MutY family
  • 組織特異性：
    Widely expressed with highest levels in heart and lowest levels in lung and liver.
  • 數據庫鏈接：

    HGNC: 8028

    OMIM: 602656

    KEGG: hsa:4913

    STRING: 9606.ENSP00000219066

    UniGene: Hs.66196



主站蜘蛛池模板： 久久国产精久久精产国| 人人摸人人搞人人透| 麻豆精品久久久久久久99蜜桃| 欧美日韩国产精品自在自线| 人妻无码一区二区三区tv| 男男女女爽爽爽免费视频| 国产公开久久人人97超碰| 中文毛片无遮挡高潮免费| h肉动漫无码无修6080动漫网| 成人性做爰aaa片免费看| 天天澡天天揉揉av无码| 国产成年无码久久久免费| 久久久久久久97| 极品少妇hdxx麻豆hdxx| 曰本大码熟中文字幕| 久久久国产精品无码一区二区| 色综合久久久无码中文字幕波多 | 亚洲国产码专区| 婷婷色婷婷开心五月四房播播| 天堂中文在线资源| 无码av一区二区大桥久未| 国产精品 中文字幕 亚洲 欧美| 欧美群交射精内射颜射潮喷| 久久国产成人午夜av影院| 久久久久人妻一区二区三区vr | 亚洲久久中文字幕www网站| 人人妻碰人人免费| 日本japanese漂亮丰满| 中国少妇内射xxxx狠干| 中文在线а√天堂官网| 国产欧美日韩综合在线成| 无码潮喷a片无码高潮视频| 风韵少妇性饥渴推油按摩视频| 久久精品女人天堂av | 国产成人午夜无码电影在线观看| 国产精品自在拍在线拍| 亚洲最大av无码国产| 国产成人精品久久一区二区| 亚洲欧洲日产国码无码| 欧美熟妇另类久久久久久不卡| 欧美高清精品一区二区|