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  4. NCOA1 Recombinant Monoclonal Antibody

NCOA1 Recombinant Monoclonal Antibody

  • 中文名稱：
    NCOA1 Recombinant Monoclonal Antibody
  • 貨號：
    CSB-RA342667A0HU
  • 規(guī)格：
    ￥1320
  • 圖片：
    • IHC image of CSB-RA342667A0HU diluted at 1:100 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • IHC image of CSB-RA342667A0HU diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • Overlay Peak curve showing PC-3 cells stained with CSB-RA342667A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for?10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他：

產品詳情

  • Uniprot No.：
  • 基因名：
  • 別名：
    Nuclear receptor coactivator 1 (NCoA-1) (EC 2.3.1.48) (Class E basic helix-loop-helix protein 74) (bHLHe74) (Protein Hin-2) (RIP160) (Renal carcinoma antigen NY-REN-52) (Steroid receptor coactivator 1) (SRC-1), NCOA1, BHLHE74 SRC1
  • 反應種屬：
    Human
  • 免疫原：
    A synthesized peptide from human NCOA1 protein
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    10C12
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 產品提供形式：
    Liquid
  • 應用范圍：
    ELISA, IHC, FC
  • 推薦稀釋比：
    Application Recommended Dilution
    IHC 1:50-1:200
    FC 1:50-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能：
    Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Involved in the coactivation of different nuclear receptors, such as for steroids (PGR, GR and ER), retinoids (RXRs), thyroid hormone (TRs) and prostanoids (PPARs). Also involved in coactivation mediated by STAT3, STAT5A, STAT5B and STAT6 transcription factors. Displays histone acetyltransferase activity toward H3 and H4; the relevance of such activity remains however unclear. Plays a central role in creating multisubunit coactivator complexes that act via remodeling of chromatin, and possibly acts by participating in both chromatin remodeling and recruitment of general transcription factors. Required with NCOA2 to control energy balance between white and brown adipose tissues. Required for mediating steroid hormone response. Isoform 2 has a higher thyroid hormone-dependent transactivation activity than isoform 1 and isoform 3.
  • 基因功能參考文獻：
    1. Our findings directly associate the AR/NCOA1 complex with PRKD1 regulation and cellular migration and support the concept of therapeutic inhibition of NCOA1 in prostate cancer. PMID: 27255895
    2. SRC-1 can impact osteoblast function in an estrogen receptor-independent manner. PMID: 28286232
    3. The pregnane X receptor down-regulates organic cation transporter 1 (SLC22A1) in human hepatocytes by competing for ("squelching") SRC-1 coactivator. PMID: 26920453
    4. miR-4443 acts in a tumor-suppressive manner by down-regulating TRAF4 and NCOA1 downstream of MEK-C/EBP-mediated leptin and insulin signaling, and that insulin and/or leptin resistance (e.g. in obesity) may suppress this pathway and increase the risk of metastatic CRC. PMID: 27842582
    5. Results provide the first evidence that NCOA1 is a direct target of miR-105-1 suggesting that NCOA1 and miR-105-1 may have potential prognostic value and may be useful as tumor biomarkers for the diagnosis of HCC patients. PMID: 28060733
    6. Our study indicated that genotype and allele frequencies of rs79480871 showed strong associations with MM patients (pa = 3.5x10-4 and pa = 1.5x10-4), and the rs6457327 genotype was more readily associated with MM patients than with controls PMID: 28264017
    7. The mechanism of IL-6-induced AR activation is mediated through enhancing AR-SRC-1 interaction and inhibiting AR-SMRT interaction. PMID: 28396297
    8. IL-6-mediated AR antagonism induced by cypermethrin is related to repress the recruitment of co-regulators SRC-1 and SMRT to the AR in a ligand-independent manner PMID: 27239967
    9. Report novel PAX3-NCOA1 gene fusions in biphenotypic sinonasal sarcomas with focal rhabdomyoblastic differentiation. PMID: 26371783
    10. Data indicate that finerenone inhibits mineralocorticoid receptor (MR), steroid receptor coactivator-1 binding at the regulatory sequence. PMID: 26203193
    11. SRC-1 polymorphisms may be the underlying cause of coronary artery aneurysms in children with Kawasaki disease. PMID: 24652666
    12. Data show that disrupting the steroid receptor coactivator-1 (SRC1) binding site on retinoid X receptor alpha (RXRalpha) alters the transactivation by CAR:RXR. PMID: 25053412
    13. NCOA1 plays a necessary role in E2-induced CXCL12 expression and NCOA2 is required for P4 to inhibit the E2-induced CXCL12 production in normal and ectopic endometrium. PMID: 24586072
    14. SRC1 and Twist1 expression are associated with poor survival in breast cancer. PMID: 24719557
    15. SRC-1 responds to cellular energy stress in tumor cells to promote the expression of genes responsible for oxidative phosphorylation. PMID: 24438340
    16. In a cohort of 453 human breast tumors, NCOA1 and CSF1 levels correlated positively with disease recurrence, higher tumor grade, and poor prognosis. PMID: 24769444
    17. IL-6 secreted by ovarian cancer cells may contribute to the refractoriness of these cells to tamoxifen via the estrogen receptor alpha isoform and SRC-1 (NCOA1). PMID: 24189439
    18. Elucidation of how SRC-1 controls transcription in breast cancers. PMID: 24648347
    19. Overexpression of SRC-1 (NCOA1) and TIF-2 (NCOA2) increases estrogen-induced gene expression. PMID: 12403846
    20. Binding of the ERalpha and ARNT1 AF2 domains to exon 21 of the SRC1 isoform SRC1e is essential for estrogen- and dioxin-transcription. PMID: 22328528
    21. Our data demonstrate that SIP is a novel regulator in caspase-independent and AIF-mediated apoptosis. PMID: 22371500
    22. We have identified a functional genetic variant of SRC-1 with decreased activity, resulting, at least in part, from the loss of a GSK3beta phosphorylation site, which was also associated with decreased bone mineral density in tamoxifen-treated women. PMID: 22174377
    23. findings suggest that SRC-1 switches steroid-responsive tumors to a steroid-resistant state in which the SRC-1 target gene ADAM22 has a critical role PMID: 22072566
    24. The induced AF1 conformation facilitates its interaction with SRC-1, and subsequent AF1-mediated transcriptional activity. PMID: 21760925
    25. NR box-2 and -3 are the essential binding targets for the SRC-1-induced stimulation of LXR transactivity; the competitive in vitro binding of NR box-2 and -3 to LXR was observed. PMID: 20682316
    26. HOXC11 and SRC-1 cooperate to regulate expression of the calcium-binding protein S100beta in resistant breast cancer cells. PMID: 20145129
    27. Studies expand knowledge of PAX3 variant translocations in rhabdomyossarcoma with identification of a novel PAX3-NCOA2 fusion. PMID: 19953635
    28. Clomiphene citrate may inhibit E2-induced endometrial epithelial cell proliferation and ERE transactivation by inhibiting the recruitment of SRC-1 to ER alpha. PMID: 19934375
    29. describes in detail the BRET(2) assay as it is used to examine the physical interaction between the nuclear receptor ERalpha and the transcriptional coactivator SRC-1 PMID: 19763509
    30. CBP/p300 and NcoA/SRC1a may function in a common pathway to regulate STAT3 transcriptional activity. PMID: 11773079
    31. Reduction of coactivator expression by antisense oligodeoxynucleotides inhibits ERalpha transcriptional activity and MCF-7 proliferation PMID: 11818499
    32. CAR antagonizes ER-mediated transcriptional activity by squelching limiting amounts of p160 coactivators, such as SRC-1 and GRIP-1. PMID: 12114525
    33. mutation of the AF2 transactivation domain on Glucocorticoid Receptors disrupts the direct interaction of GR with steroid receptor coactivator 1 (SRC-1) PMID: 12118039
    34. STAT6 has a protein binding motif that controls the interaction with NcoA-1 in transcriptional activation PMID: 12138096
    35. Data show that steroid receptor coactivator-1 (SRC-1) enhanced ligand-independent activation of the AR by IL-6 to the same magnitude as that obtained via ligand-dependent activation, and that activation required MAPK. PMID: 12163482
    36. our results indicate an important role for Mediator, as well as its functional interplay with p300/CBP-SRC, in the enhancement of ERalpha-dependent transcription with chromatin templates PMID: 12482985
    37. Sumoylation was shown to increase progesterone receptor-SRC-1 interaction and to prolong SRC-1 retention in the nucleus. PMID: 12529333
    38. is a coactivator of MHC class II genes that stimulates their interferon gamma (IFNgamma) and class II transactivator (CIITA)-mediated expression. PMID: 12933903
    39. NCoA-1/SRC-1 is an essential coactivator of STAT5 that binds to the FDL motif in the alpha-helical region of the STAT5 transactivation domain PMID: 12954634
    40. differential recruitment of steroid receptor coactivator-1 and silencing mediator for retinoid and thyroid receptors by estrogen receptor-alpha and beta in breast cancer may be central to the response of the tumor to endocrine treatment PMID: 14715875
    41. surface complementarity between the hydrophobic faces of the STAT6 fragment and of the NCoA-1 PAS-B domain almost exclusively defines the binding specificity between the two proteins PMID: 14757047
    42. TNF-alpha impairs progesterone-stimulated PR-B-mediated transactivation, and these effects appear to be due, in part, to reduced expression of SRC-1 and -2 PMID: 15231721
    43. A novel translocation t(2;2)(q35;p23), which generates a fusion protein composed of PAX3 and the NCOA1was identified in rhabdomyosarcoma. PMID: 15313887
    44. Data show that steroid receptor coactivator-1 (SRC-1) overexpressing cells are more responsive to Po mRNA induction by dihydroprogesterone (DHP) than SRC-1-deficient cells, and that DHP treatment increases not only Po but also SRC-1 mRNA levels. PMID: 15456935
    45. The conserved STAT3 region from 752 to 761, called STAT3 CR2, plays critical roles in STAT3-dependent transcription by recruiting SRC-1 and allowing Ser727 phosphorylation. PMID: 15530426
    46. The SRC-1 has been shown to be involved in HIF-1alpha-mediated activation of transcription. PMID: 15615775
    47. SRC-1 and SMRT content change in a tissue-specific manner in the rat brain during the estrous cycle. PMID: 15862975
    48. ASXL1 is a novel coactivator of RAR that cooperates with SRC-1 PMID: 16606617
    49. SRC1-deficient P19 cells showed severely compromised retinoid-induced responses, in agreement with the supposed role of SRC1 as a retinoic acid recepetor coactivator. PMID: 16723356
    50. HPV E7 proteins dysregulate hormone-dependent gene expression by association with and relocalization of SRC-1. PMID: 16775354

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  • 相關疾病：
    A chromosomal aberration involving NCOA1 is a cause of rhabdomyosarcoma. Translocation t(2;2)(q35;p23) with PAX3 generates the NCOA1-PAX3 oncogene consisting of the N-terminus part of PAX3 and the C-terminus part of NCOA1. The fusion protein acts as a transcriptional activator. Rhabdomyosarcoma is the most common soft tissue carcinoma in childhood, representing 5-8% of all malignancies in children.
  • 亞細胞定位：
    Nucleus.
  • 蛋白家族：
    SRC/p160 nuclear receptor coactivator family
  • 組織特異性：
    Widely expressed.
  • 數據庫鏈接：

    HGNC: 7668

    OMIM: 602691

    KEGG: hsa:8648

    STRING: 9606.ENSP00000320940

    UniGene: Hs.596314



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