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  4. HTATSF1 Recombinant Monoclonal Antibody

HTATSF1 Recombinant Monoclonal Antibody

  • 中文名稱：
    HTATSF1 Recombinant Monoclonal Antibody
  • 貨號：
    CSB-RA824594A0HU
  • 規格：
    ￥1320
  • 圖片：
    • Western Blot
      Positive WB detected in: Jurkat whole cell lysate(30μg)
      All lanes: Tat SF1 antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/40000 dilution
      Predicted band size: 86 kDa
      Observed band size: 140 kDa
      Exposure time：3min
    • IHC image of CSB-RA824594A0HU diluted at 1:100 and staining in paraffin-embedded human gastric cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • IHC image of CSB-RA824594A0HU diluted at 1:100 and staining in paraffin-embedded human thyroid tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • Immunofluorescence staining of Hela cell with CSB-RA824594A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

產品詳情

  • Uniprot No.：
  • 基因名：
    HTATSF1
  • 別名：
    HIV Tat-specific factor 1 (Tat-SF1), HTATSF1
  • 反應種屬：
    Human
  • 免疫原：
    A synthesized peptide from human HTATSF1 protein
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    6B7
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 產品提供形式：
    Liquid
  • 應用范圍：
    ELISA, WB, IHC, IF
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:2000
    IHC 1:50-1:200
    IF 1:50-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能：
    Functions as a general transcription factor playing a role in the process of transcriptional elongation. May mediate the reciprocal stimulatory effect of splicing on transcriptional elongation. In case of infection by HIV-1, it is up-regulated by the HIV-1 proteins NEF and gp120, acts as a cofactor required for the Tat-enhanced transcription of the virus.
  • 基因功能參考文獻：
    1. This study validates Tat-SF1 as an HDF in CD4+ T cell-derived SupT1 cells. PMID: 23153325
    2. these findings suggest that Tat-SF1 functions independently in transcription and splicing of cellular genes. PMID: 21282347
    3. These results support the notion that Tat-induced neuropathogenesis is mediated by multiple mechanisms involving both intracellular and extracellular Tat protein. PMID: 15050687
    4. phosphorylated by P-TEFb kinase during HIV-1 transcription in Tat/TAR dependent manner PMID: 15564463
    5. Intracellular HIV-1 Tat protein represses constitutive LMP2 transcription increasing proteasome activity by interfering with the binding of IRF-1 to STAT1 PMID: 16512786
    6. Tat-SF1 is not required for regulating HIV-1 transcription, but is required for maintaining the ratios of different classes of HIV-1 transcripts. PMID: 19479034

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  • 亞細胞定位：
    Nucleus.
  • 蛋白家族：
    HTATSF1 family
  • 組織特異性：
    Widely expressed.
  • 數據庫鏈接：

    HGNC: 5276

    OMIM: 300346

    KEGG: hsa:27336

    STRING: 9606.ENSP00000218364

    UniGene: Hs.204475



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