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FPGS Recombinant Monoclonal Antibody

  • 中文名稱：
    FPGS重組抗體
  • 貨號(hào)：
    CSB-RA983477A0HU
  • 規(guī)格：
    ￥1320
  • 圖片：
    • Overlay Peak curve showing HepG2 cells stained with CSB-RA983477A0HU (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1μg/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1μg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    CSB-RA983477A0HU FPGS重組單克隆抗體是針對(duì)葉酰聚谷氨酸合成酶（Folylpolyglutamate Synthetase）研發(fā)的高特異性檢測工具。該抗體靶向的FPGS是葉酸代謝通路中的關(guān)鍵酶，負(fù)責(zé)催化葉酸及其衍生物的多聚谷氨酸化反應(yīng)，該過程直接影響細(xì)胞內(nèi)葉酸的有效滯留及部分抗腫瘤藥物的活化代謝。經(jīng)嚴(yán)格驗(yàn)證，本產(chǎn)品在ELISA和流式細(xì)胞術(shù)（FC）中表現(xiàn)出優(yōu)異的檢測性能，其中FC實(shí)驗(yàn)推薦使用1:50至1:200的稀釋比例，能夠精準(zhǔn)識(shí)別內(nèi)源性FPGS蛋白的表達(dá)水平。適用于探索葉酸依賴性代謝調(diào)控機(jī)制、腫瘤細(xì)胞增殖與凋亡研究，以及化療藥物（如甲氨蝶呤）耐藥性相關(guān)的分子機(jī)制解析等科研領(lǐng)域。其重組單克隆特性確保了批次間的高度一致性，為細(xì)胞生物學(xué)、分子藥理學(xué)等基礎(chǔ)研究提供穩(wěn)定可靠的檢測支持。
  • Uniprot No.：
  • 基因名：
  • 別名：
    Folylpolyglutamate synthase, mitochondrial (EC 6.3.2.17) (Folylpoly-gamma-glutamate synthetase) (FPGS) (Tetrahydrofolylpolyglutamate synthase) (Tetrahydrofolate synthase), FPGS
  • 反應(yīng)種屬：
    Human
  • 免疫原：
    A synthesized peptide derived from Human FPGS
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標(biāo)記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號(hào)：
    30C11
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, FC
  • 推薦稀釋比：
    Application Recommended Dilution
    FC 1:50-1:200
  • Protocols：
  • 儲(chǔ)存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Catalyzes conversion of folates to polyglutamate derivatives allowing concentration of folate compounds in the cell and the intracellular retention of these cofactors, which are important substrates for most of the folate-dependent enzymes that are involved in one-carbon transfer reactions involved in purine, pyrimidine and amino acid synthesis. Unsubstituted reduced folates are the preferred substrates. Metabolizes methotrexate (MTX) to polyglutamates.
  • 基因功能參考文獻(xiàn)：
    1. The association of aberrant folylpolyglutamate synthetase splicing with ex vivo methotrexate resistance and clinical outcome in childhood acute lymphoblastic leukemia. PMID: 27036162
    2. Low expression level of FPGS is associated with neuroendocrine lung tumors. PMID: 27064343
    3. Our results revealed a significant difference of overall survival between patients with AA genotype and GA + GG genotype (P<0.05). Our study demonstrated a correlation of the polymorphism of FPGS rs1544105 with MTX treatment efficacy, as well as with overall survival, suggesting it could be used as an effective approach in the prediction of MTX treatment efficacy PMID: 27987364
    4. These results indicate that elevated expressions of the tumor-related genes FPGS/GGH and VEGF are correlated with malignancy of thymic carcinoma and B3 thymoma tumors. PMID: 27387303
    5. 529 adults (n = 325 European-Americans, 204 Egyptians) on a stable warfarin dose were genotyped for GGCX rs12714145 and rs10654848, FPGS rs7856096, and STX1B rs4889606. PMID: 26751406
    6. data suggest that FPGS modulation affects global and promoter CpG DNA methylation and expression of several genes involved in important biological pathways PMID: 26895662
    7. FPGS and ABCB1 genetic variants can influence the outcome in RA patients receiving MTX monotherapy. PMID: 26652611
    8. Genetic polymorphisms in the FPGS coding region are significantly associated with increased Acute Lymphoblastic Leukemia risk. PMID: 26107232
    9. study found that genetic polymorphism of FPGS rs10760502A > G is associated with susceptibility to primary retroperitoneal liposasrcoma PMID: 25765001
    10. antifolate resistant leukemia cells harbor a heterozygous point mutation in exon12 of FPGS which disrupts FPGS activity by abolishing ATP binding, and alters the binding pattern of transcription factors to the genomic region of exon12. PMID: 25229333
    11. Genome-wide association studies identify 10 novel genetic loci as risk factors for methotrexate response in rheumatoid arthritis patients additionally to polymorphism in DHFR, FPGS and TYMS. PMID: 24583629
    12. mitochondrial FPGS is required because folate polyglutamates are not substrates for transport across the mitochondrial membrane PMID: 25164808
    13. Polymorphism of FPGS rs1544105 G>A is associated with response to therapy in acute lymphoblastic leukemia. PMID: 24908438
    14. An interaction term, between FPGS rs7033913 heterozygotes and GGH rs11988534 homozygotes for the minor allele, had a p-value <0.0001 and may contribute to methotrexate toxicity in rheumatoid arthritis. PMID: 24447348
    15. High Folylpoly-glutamate synthetase expression is associated with response to therapy in non-small-cell lung cancer. PMID: 22895141
    16. A novel missense mutation 502/490 T>C (L151/101P) in exon 5 of FPGS gene is firstly found in Chinese Han children, and the cytosolic and mitochondrial variants display allelic frequencies of 0.70 % and 0.47 % respectively. PMID: 22809608
    17. The mbr enhanced the expression of FPGS significantly and increased sensitivity of Jurkat cells to MTX efficiently. PMID: 22698741
    18. This is one of the first studies to assess FPGS and GGH genetic variants in relation to plasma homocysteine. PMID: 22018726
    19. The expression of mFPGS was different in A549 cells exposed to different methotrexate enantiomers. PMID: 21418912
    20. intratumor FPGS plays an important role in the efficacy of oral fluoropyrimidine plus LV therapy for colorectal cancer. PMID: 21380490
    21. FPGS gene is proposed as a novel causative gene for cleft lip. PMID: 21093159
    22. Data revealed that high FPGS gene expression, low GGH gene expression and low ABCC1 gene expression in CRC tissues were predictive factors for a high reduced folate level after LV administration. PMID: 19636555
    23. low folylpolyglutamate synthase expression is associated with development of aggressive metastatic behavior of the colorectal cancer PMID: 14676127
    24. Significantly higher expression levels of FPGS were found in mucosa expressing the splice variant DCC342. PMID: 16122883
    25. human mitochondrial FPGS is at least in part strongly associated with the inner mitochondrial membrane PMID: 16169100
    26. Investigation of the molecular regulatory mechanisms directing FPGS gene expression in B precursor-ALL and T-ALL cells. PMID: 16707018
    27. study identified FPGS polymorphisms; results indicate that two of the cytosolic nonsynonymous coding SNPs affected protein expression, in vitro substrate kinetics & folate & antifolate efficacy in cells expressing the polymorphisms PMID: 17875718
    28. Results indicate that hFPGS can catalyze the processive addition of approximately four glutamate residues to DDAH 4PteGlu 1. PMID: 18672898
    29. Aberrant splicing of folylpolyglutamate synthetase is associated with antifolate resistance in leukemia. PMID: 19131550

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  • 亞細(xì)胞定位：
    [Isoform 1]: Mitochondrion inner membrane. Mitochondrion matrix.; [Isoform 2]: Cytoplasm.
  • 蛋白家族：
    Folylpolyglutamate synthase family
  • 數(shù)據(jù)庫鏈接：

    HGNC: 3824

    OMIM: 136510

    KEGG: hsa:2356

    STRING: 9606.ENSP00000362344

    UniGene: Hs.335084



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