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  4. EIF4A3 Recombinant Monoclonal Antibody

EIF4A3 Recombinant Monoclonal Antibody

  • 中文名稱：
    EIF4A3 Recombinant Monoclonal Antibody
  • 貨號：
    CSB-RA965999A0HU
  • 規格：
    ￥1320
  • 圖片：
    • Immunofluorescence staining of Hela cell with CSB-RA965999A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Overlay Peak curve showing jurkat cells stained with CSB-RA965999A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for?10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他：

產品詳情

  • Uniprot No.：
  • 基因名：
    EIF4A3
  • 別名：
    ATP-dependent RNA helicase DDX48 antibody; ATP-dependent RNA helicase eIF4A-3 antibody; DDX48 antibody; DEAD box protein 48 antibody; eIF-4A-III antibody; eIF4A-III antibody; EIF4A3 antibody; eIF4AIII antibody; Eukaryotic initiation factor 4A-III antibody; Eukaryotic initiation factor 4A-like NUK-34 antibody; Eukaryotic translation initiation factor 4A isoform 3 antibody; hNMP 265 antibody; IF4A3_HUMAN antibody; NMP 265 antibody; NMP265 antibody; Nuclear matrix protein 265 antibody; NUK34 antibody
  • 反應種屬：
    Human
  • 免疫原：
    A synthesized peptide derived from human EIF4A3
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    6E9
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
  • 產品提供形式：
    Liquid
  • 應用范圍：
    ELISA, IF, FC
  • 推薦稀釋比：
    Application Recommended Dilution
    IF 1:50-1:200
    FC 1:50-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能：
    ATP-dependent RNA helicase. Involved in pre-mRNA splicing as component of the spliceosome. Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). Its RNA-dependent ATPase and RNA-helicase activities are induced by CASC3, but abolished in presence of the MAGOH-RBM8A heterodimer, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. The inhibition of ATPase activity by the MAGOH-RBM8A heterodimer increases the RNA-binding affinity of the EJC. Involved in translational enhancement of spliced mRNAs after formation of the 80S ribosome complex. Binds spliced mRNA in sequence-independent manner, 20-24 nucleotides upstream of mRNA exon-exon junctions. Shows higher affinity for single-stranded RNA in an ATP-bound core EJC complex than after the ATP is hydrolyzed. Involved in the splicing modulation of BCL2L1/Bcl-X (and probably other apoptotic genes); specifically inhibits formation of proapoptotic isoforms such as Bcl-X(S); the function is different from the established EJC assembly. Involved in craniofacial development.
  • 基因功能參考文獻：
    1. FAL1 may work as a ceRNA to modulate AKT1 expression via competitively binding to miR-637 in HSCR. PMID: 30062828
    2. we firstly report that the lncRNA H19 recruits eIF4A3 and promotes the colorectal cancer cell proliferation PMID: 26989025
    3. High EIF4A expression is associated with malignant peripheral nerve sheath tumors and vestibular schwannomas. PMID: 26951381
    4. MYC, DUX4, and EIF4A3 might contribute to facioscapulohumeral dystrophy pathophysiology PMID: 28273136
    5. Host eIF4AIII RNA helicase is required for efficient human cytomegalovirus replication. PMID: 26773380
    6. EIF4A3 is a gene involved in RNA metabolism, plays a role in mandible, laryngeal, and limb morphogenesis. Richieri-Costa-Pereira syndrome is caused by mutations in EIF4A3. PMID: 24360810
    7. The binding mode of CWC22 to eIF4AIII reveals a facet of how MIF4G domains use their versatile structural frameworks to activate or inhibit DEAD-box proteins. PMID: 24218557
    8. CWC22 escorts the helicase eIF4AIII to spliceosomes and promotes exon junction complex assembly PMID: 22961380
    9. demonstrate direct physical interactions between yeast Sgd1p and Fal1p, and between their human orthologs (NOM1 and eIF4AIII) in vitro and in vivo, identifying human NOM1 as a missing eIF4G-like interacting partner of eIF4AIII PMID: 21576267
    10. identification of eIF4AIII and Barentsz as components of a conserved protein complex that is essential for mRNA localization in flies and nonsense-mediated mRNA decay in mammals PMID: 14973490
    11. eIF4AIII represents a new functional class of DExH/D box proteins that act as RNA clamps or 'place holders' for the sequence-independent attachment of additional factors to RNAs. PMID: 15034551
    12. detection of autoantibodies to DDX48 may have clinical utility for the improved diagnosis of pancreatic cancer PMID: 15796914
    13. The stable association of multiprotein exon junction complex core with RNA is maintained by inhibition of eIF4AIII ATPase activity by MAGOH-Y14. PMID: 16170325
    14. crystal structure of a tetrameric exon junction core complex containing the DEAD-box adenosine triphosphatase eukaryotic initiation factor 4AIII bound to an ATP analog, MAGOH, Y14, a fragment of MLN51, and a polyuracil mRNA mimic PMID: 16931718
    15. Although it is dispensable for pre-mRNA splicing in vitro, eIF4A3 is required for splicing-dependent loading of the Y14-Magoh core heterodimer onto mRNA. PMID: 17606899
    16. Data show that eIF4AIII, a core exon junction complex (EJC) component loaded onto mRNAs by pre-mRNA splicing, is associated with neuronal mRNA granules and dendritic mRNAs. PMID: 17632064
    17. The nonsense-mediated-decay mRNA surveillance pathway downregulates aberrant E-cadherin transcripts in gastric cancer cells and in CDH1 mutation carriers. PMID: 18427545
    18. NMP 265, a common part of the nuclear matrix, is located in the nucleus via an N-terminal amino acid sequence. PMID: 10623621

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  • 相關疾病：
    Richieri-Costa-Pereira syndrome (RCPS)
  • 亞細胞定位：
    Nucleus. Nucleus speckle. Cytoplasm.
  • 蛋白家族：
    DEAD box helicase family, eIF4A subfamily
  • 組織特異性：
    Ubiquitously expressed.
  • 數據庫鏈接：

    HGNC: 18683

    OMIM: 268305

    KEGG: hsa:9775

    STRING: 9606.ENSP00000269349

    UniGene: Hs.389649



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