Recombinant Mouse Non-specific lipid-transfer protein (Scp2)

說(shuō)明書(shū)

中文名稱(chēng)：

小鼠Scp2重組蛋白
貨號(hào)：

020856MO
規(guī)格：
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
靶點(diǎn)詳情

產(chǎn)品詳情

Uniprot No.：

P32020
基因名：

Scp2
別名：

Scp2; Scp-2; Non-specific lipid-transfer protein; NSL-TP; EC 2.3.1.176; Propanoyl-CoA C-acyltransferase; SCP-chi; SCPX; Sterol carrier protein 2; SCP-2; Sterol carrier protein X; SCP-X
免疫原種屬：

Mus musculus (Mouse)
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
應(yīng)用說(shuō)明：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
儲(chǔ)存條件：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

Plays a crucial role in the peroxisomal oxidation of branched-chain fatty acids. Catalyzes the last step of the peroxisomal beta-oxidation of branched chain fatty acids and the side chain of the bile acid intermediates di- and trihydroxycoprostanic acids (DHCA and THCA). Also active with medium and long straight chain 3-oxoacyl-CoAs. Stimulates the microsomal conversion of 7-dehydrocholesterol to cholesterol and transfers phosphatidylcholine and 7-dehydrocholesterol between membrances, in vitro. Isoforms SCP2 and SCPx cooperate in peroxisomal oxidation of certain naturally occurring tetramethyl-branched fatty acyl-CoAs.; Mediates the transfer of all common phospholipids, cholesterol and gangliosides from the endoplasmic reticulum to the plasma membrane. May play a role in regulating steroidogenesis. Stimulates the microsomal conversion of 7-dehydrocholesterol to cholesterol. Also binds fatty acids and fatty acyl Coenzyme A (CoA) such as phytanoyl-CoA. Involved in the regulation phospholipid synthesis in endoplasmic reticulum enhancing the incorporation of exogenous fatty acid into glycerides. Seems to stimulate the rate-limiting step in phosphatidic acid formation mediated by GPAT3. Isoforms SCP2 and SCPx cooperate in peroxisomal oxidation of certain naturally occurring tetramethyl-branched fatty acyl-CoAs.
基因功能參考文獻(xiàn)：
1. Ablating both Fabp1 and Scp2/Scpx (TKO) induces hepatic phospholipid and cholesterol accumulation in high fat-fed mice PMID: 29307784
2. role of Fabp1/Scp-2 in hepatic phytol metabolism PMID: 28411199
3. Thus, the loss of Scp-2/Scp-x contributed to a sex-dependent hepatic accumulation of dietary phytol and BCFA. PMID: 29051070
4. Individually ablating SCPx or SCP2/SCPx elicited concomitant upregulation of L-FABP. PMID: 27940000
5. Lack of a significant decrease in the flux of HDL-[(3)H]CE to biliary FC or bile acids in FABP1(-/-) mice indicates the likely compensation of its function by an as yet unidentified mechanism. Taken together, these studies demonstrate that FABP1 and SCP2 facilitate the preferential movement of HDL-CEs to bile for final elimination PMID: 27381048
6. Sterol Carrier Protein-2, a Nonspecific Lipid-Transfer Protein, in Intracellular Cholesterol Trafficking in Testicular Leydig Cells PMID: 26901662
7. L-FABP was more important in hepatic retention of bile acids, while SCP-2/SCP-x more broadly affected biliary bile acid and phospholipid levels. PMID: 26541319
8. Loss of L-FABP and SCP-2, or both induces hepatic lipid accumulation in female mice and mimics non-alcoholic fatty liver disease. PMID: 26116377
9. SCP-2/SCP-x may function more in formation and biliary secretion of bile acid, with less impact on hepatic uptake or biliary secretion of HDL-cholesterol PMID: 25277800
10. liver fatty acid-binding protein, the single most prevalent hepatic cytosolic protein that binds cholesterol, was upregulated twofold in SCP-2 null hepatocytes PMID: 22241858
11. SCP-2 expression plays a significant role in HDL-mediated cholesterol efflux by regulating the size of rapid vs. slow cholesterol efflux pools and/or eliciting concomitant upregulation of L-FABP in cultured primary hepatocytes. PMID: 20395534
12. Effect of Scp2 expression on the rate of sterol transfer from lysosomal membranes, and lysosomal membrane lipid distribution. PMID: 12093533
13. Data show that expression of sterol carrier protein-2 (SCP-2) increased fatty acid uptake and targeted fatty acid to unique lipid pools, suggesting that SCP-2 may effectively serve as universal fatty acid binding and trafficking protein. PMID: 12479573
14. Results suggest a key regulatory role for sterol carrier protein-2 in hepatic lipid metabolism. PMID: 12576522
15. SCP-2 contributes to the accumulation of cholesterol in L-FABP null liver PMID: 12670956
16. SCP-2 and SCP-x stimulate oxidation and esterification of branched-chain as well as straight-chain fatty acids in intact cells. PMID: 12810824
17. Results demonstrate a direct physiological relationship between lack of SCP-x and decreased ability to metabolize branched-chain lipids. PMID: 17068117
18. Regulation of the SCPx gene by SF-1 and cAMP is similar to the regulatory mechanisms observed for other steroidogenic genes. PMID: 17434450
19. SCP-2/SCP-x has a role in regulating lipid raft domains in primary cultured mouse hepatocytes PMID: 17609524
20. Results support an important role for SCP-2 in hepatic cholesterol homeostasis. PMID: 19289417
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亞細(xì)胞定位：

[Isoform SCP2]: Cytoplasm. Peroxisome. Endoplasmic reticulum. Mitochondrion.; [Isoform SCPx]: Peroxisome.
蛋白家族：

Thiolase family
組織特異性：

Present at low levels in all tissues examined but expressed predominantly in the liver.; [Isoform SCPx]: In testis, highly expressed in the interstitial connective tissue, an area rich in interstitial cells of Leydig, but it is barely expressed in the ger
數(shù)據(jù)庫(kù)鏈接：

KEGG: mmu:20280

STRING: 10090.ENSMUSP00000030340

UniGene: Mm.379011