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  4. VPS39 Antibody

VPS39 Antibody

  • 中文名稱：
    VPS39兔多克隆抗體
  • 貨號：
    CSB-PA836235LA01HU
  • 規格：
    ￥440
  • 圖片：
    • Western Blot
      Positive WB detected in: Hela whole cell lysate(20μg), U251 whole cell lysate(20μg), 293 whole cell lysate(20μg)
      All lanes: VPS39 antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 102 kDa
      Observed band size: 102 kDa
      Exposure time：120s
    • Immunofluorescence staining of A549 cell with CSB-PA836235LA01HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Immunofluorescence staining of A549 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Immunofluorescence staining of Hela cell with CSB-PA836235LA01HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Immunofluorescence staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • IHC image of CSB-PA836235LA01HU diluted at 1:300 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody
  • 其他：

產品詳情

  • 產品描述：

    The recombinant peptide corresponding to amino acids 263-490 of the human VPS39 is used as the immunogen to immunize the rabbit to yield the anti-VPS39 antibody. The VPS39 polyclonal antibody exists as an unconjugated IgG isoform. Its purity is 95%+ using protein G purified. It reacts with human and mouse VPS39. This VPS39 antibody has been validated for use in ELISA, WB, IF, and IHC analyses.

    VPS39 mainly regulates endosomal membrane trafficking and fusion. It is a subunit of the homotypic fusion and protein sorting (HOPS) complex, which is involved in the tethering and fusion of endosomes and lysosomes. It is also involved in the regulation of lysosomal biogenesis, autophagy, and signaling pathways. Mutations in the VPS39 gene have been linked to various diseases such as Hermansky-Pudlak syndrome.

  • 產品名稱：
    Rabbit anti-Homo sapiens (Human) VPS39 Polyclonal antibody
  • Uniprot No.：
  • 基因名：
  • 別名：
    A230065P22Rik antibody; AW743070 antibody; FLJ21681 antibody; FLJ46546 antibody; hVam6p antibody; KIAA0770 antibody; RP23-348L16.12 antibody; TLP antibody; TRAP1 like protein antibody; Vacuolar protein sorting 39 antibody; Vacuolar protein sorting 39 homolog antibody; VAM6 antibody; Vam6/Vps39 like antibody; Vam6/Vps39 like protein antibody; Vam6/Vps39-like protein antibody; VPS 39 antibody; Vps39 antibody; VPS39_HUMAN antibody
  • 宿主：
    Rabbit
  • 反應種屬：
    Human
  • 免疫原：
    Synthesized peptide derived from human VPS39
  • 免疫原種屬：
    Homo sapiens (Human)
  • 克隆類型：
    Polyclonal
  • 抗體亞型：
    IgG
  • 純化方式：
    Antigen affinity purification
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
  • 產品提供形式：
    Liquid
  • 應用范圍：
    ELISA, WB, IHC, IF
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:1000-1:3000
    IHC 1:200-1:500
    IF 1:20-1:100
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能：
    Regulator of TGF-beta/activin signaling, inhibiting SMAD3- and activating SMAD2-dependent transcription. Acts by interfering with SMAD3/SMAD4 complex formation, this would lead to inhibition of SMAD3-dependent transcription and relieve SMAD3 inhibition of SMAD2-dependent promoters, thus increasing SMAD2-dependent transcription. Does not affect TGF-beta-induced SMAD2 or SMAD3 phosphorylation, nor SMAD2/SMAD4 complex formation.; Plays a role in vesicle-mediated protein trafficking to lysosomal compartments including the endocytic membrane transport and autophagic pathways. Acts as a component of the putative HOPS endosomal tethering complex which is proposed to be involved in the Rab5-to-Rab7 endosome conversion probably implicating MON1A/B, and via binding SNAREs and SNARE complexes to mediate tethering and docking events during SNARE-mediated membrane fusion. The HOPS complex is proposed to be recruited to Rab7 on the late endosomal membrane and to regulate late endocytic, phagocytic and autophagic traffic towards lysosomes. Involved in homotypic vesicle fusions between late endosomes and in heterotypic fusions between late endosomes and lysosomes. Required for fusion of endosomes and autophagosomes with lysosomes.
  • 基因功能參考文獻：
    1. TLP expression contributes to hypertrophic scar formation and contraction. PMID: 25655281
    2. TLP likely acts as a molecular modulator capable of altering the balance of Smad3- and Smad2-dependent signaling through regulation of phosphorylation, thus facilitating collagen synthesis in fibroblasts. PMID: 23418473
    3. Vps39 knockdown impairs late endosome fusion and fusion between late endosomes and lysosomes. PMID: 23167963
    4. Merkel cell polyomavirus large T antigen disrupts lysosome clustering by translocating human Vam6p from the cytoplasm to the nucleus PMID: 21454559
    5. although only TBC1D15/Rab7-GAP altered Rab7-GTP levels, both Rab7-GAP and mVps39 regulate lysosomal morphology and play a role in maintaining growth factor dependence PMID: 20363736
    6. propose that TLP might regulate the balance of Smad2 and Smad3 signaling by localizing Smad4 intracellularly, thus contributing to cellular specificity of TGF-beta transcriptional responses in both normal and pathophysiology PMID: 12941698

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  • 亞細胞定位：
    Cytoplasm. Lysosome membrane; Peripheral membrane protein. Late endosome membrane; Peripheral membrane protein.;
  • 蛋白家族：
    VAM6/VPS39 family
  • 組織特異性：
    Widely expressed, with highest levels in heart, skeletal muscle, kidney, pancreas, brain, placenta and spleen.
  • 數據庫鏈接：

    HGNC: 20593

    OMIM: 612188

    KEGG: hsa:23339

    STRING: 9606.ENSP00000326534

    UniGene: Hs.88025



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