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  4. TPST1 Antibody

TPST1 Antibody

  • 中文名稱：
    TPST1兔多克隆抗體
  • 貨號：
    CSB-PA024132LA01HU
  • 規格：
    ￥3,900
  • 圖片：
    • IHC image of CSB-PA024132LA01HU diluted at 1:50 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody
    • IHC image of CSB-PA024132LA01HU diluted at 1:50 and staining in paraffin-embedded human colorectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody
    • Western Blot
      Positive WB detected in: Mouse Skeletal Muscle tissue lysate(20μg),Rat Skeletal Muscle tissue lysate(20μg)
      All lanes: TPST1 antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/40000 dilution
      Predicted band size: 43 kDa
      Observed band size: 43 kDa
      Exposure time: 120s
    • Western Blot
      Positive WB detected in: A549 whole cell lysate(20μg), U251 whole cell lysate(20μg), HepG2 whole cell lysate(20μg), Hela whole cell lysate(20μg), PC-3 whole cell lysate(20μg), 293 whole cell lysate(20μg)
      All lanes: TPST1 antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/20000 dilution
      Predicted band size: 43 kDa
      Observed band size: 43 kDa
      Exposure time: 60s
  • 其他：

產品詳情

  • 產品名稱：
    Rabbit anti-Homo sapiens (Human) TPST1 Polyclonal antibody
  • Uniprot No.：
  • 基因名：
    TPST1
  • 別名：
    TPST1; Protein-tyrosine sulfotransferase 1; Tyrosylprotein sulfotransferase 1; TPST-1
  • 宿主：
    Rabbit
  • 反應種屬：
    Human, Mouse, Rat
  • 免疫原：
    Recombinant Human Protein-tyrosine sulfotransferase 1 protein (283-370AA)
  • 免疫原種屬：
    Homo sapiens (Human)
  • 克隆類型：
    Polyclonal
  • 抗體亞型：
    IgG
  • 純化方式：
    Antigen?affinity?purification
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Preservative: 0.02% sodium azide
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 產品提供形式：
    Liquid
  • 應用范圍：
    ELISA, WB, IHC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:1000-1:3000
    IHC 1:50-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能：
    Catalyzes the O-sulfation of tyrosine residues within acidic motifs of polypeptides, using 3'-phosphoadenylyl sulfate (PAPS) as cosubstrate.
  • 基因功能參考文獻：
    1. TPST1 was significantly negatively correlated with the expression of cMet in lung cancer and may be a negative prognostic biomarker of lung cancer. PMID: 26238632
    2. TPST1 rs3757417T>G polymorphisms are associated with colorectal cancer. PMID: 25079514
    3. Affinity purified salivary TPST showed a single band of 50-54 kDa and is the first report characterizing a tyrosylprotein sulfotransferase in secretory fluid from the parotid gland PMID: 16244708
    4. results exclude TPST1 as the causative gene for Shwachman-Diamond syndrome PMID: 12032733
    5. Shear stress-dependent downregulation of TPST1 in human endothelium involves protein kinase C PMID: 12056800
    6. Human tyrosylprotein sulfotransferase may be functional as homodimer/oligomer in the trans-Golgi compartment. PMID: 16859706

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  • 亞細胞定位：
    Golgi apparatus membrane; Single-pass type II membrane protein.
  • 蛋白家族：
    Protein sulfotransferase family
  • 組織特異性：
    Ubiquitous. Detected in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
  • 數據庫鏈接：

    HGNC: 12020

    OMIM: 603125

    KEGG: hsa:8460

    STRING: 9606.ENSP00000302413

    UniGene: Hs.421194



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