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  4. SEMA4B Antibody

SEMA4B Antibody

  • 中文名稱：
    SEMA4B兔多克隆抗體
  • 貨號：
    CSB-PA868280LA01HU
  • 規格：
    ￥440
  • 圖片：
    • Western Blot
      Positive WB detected in: Rat luing tissue
      All lanes: SEMA4B antibody at 6.3μg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 93, 82 kDa
      Observed band size: 93 kDa
    • IHC image of CSB-PA868280LA01HU diluted at 1:300 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • IHC image of CSB-PA868280LA01HU diluted at 1:300 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of HepG2 cells with CSB-PA868280LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

產品詳情

  • 產品名稱：
    Rabbit anti-Homo sapiens (Human) SEMA4B Polyclonal antibody
  • Uniprot No.：
  • 基因名：
    SEMA4B
  • 別名：
    SEMA4B antibody; KIAA1745 antibody; SEMAC antibody; UNQ749/PRO1480 antibody; Semaphorin-4B antibody; Semaphorin-C antibody
  • 宿主：
    Rabbit
  • 反應種屬：
    Human, Rat
  • 免疫原：
    Recombinant Human Semaphorin-4B protein (163-253AA)
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated

    本頁面中的產品，SEMA4B Antibody (CSB-PA868280LA01HU)，的標記方式是Non-conjugated。對于SEMA4B Antibody，我們還提供其他標記。見下表：

    可提供標記
    標記方式 貨號 產品名稱 應用
    HRP CSB-PA868280LB01HU SEMA4B Antibody, HRP conjugated ELISA
    FITC CSB-PA868280LC01HU SEMA4B Antibody, FITC conjugated
    Biotin CSB-PA868280LD01HU SEMA4B Antibody, Biotin conjugated ELISA
  • 克隆類型：
    Polyclonal
  • 抗體亞型：
    IgG
  • 純化方式：
    >95%, Protein G purified
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 產品提供形式：
    Liquid
  • 應用范圍：
    ELISA, WB, IHC, IF
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:100-1:300
    IF 1:50-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能：
    Inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons.
  • 基因功能參考文獻：
    1. SEMA4B may induce FoxO1 nuclear retention through suppressing PI3K/Akt signaling pathway, which subsequently inhibited cell growth through the direct nuclear target of FoxO1, p21. PMID: 25746385
    2. Decrease in SEMA4b levels are associated with metastasis of non-small cell lung cancer. PMID: 25095981
    3. showed that HIF-1alpha recognized a hypoxia-responsive element (HRE) of SEMA4B gene, which is required for HIF-1-repressed SEMA4B expression PMID: 24474252
  • 亞細胞定位：
    Membrane; Single-pass type I membrane protein.
  • 蛋白家族：
    Semaphorin family
  • 數據庫鏈接：

    HGNC: 10730

    KEGG: hsa:10509

    STRING: 9606.ENSP00000332204

    UniGene: Hs.474935



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