在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

Mono-methyl-HIST1H1C (K186) Antibody

  • 中文名稱:
    Mono-methyl-HIST1H1C (K186)兔多克隆抗體
  • 貨號:
    CSB-PA010378PA186me1HU
  • 規(guī)格:
    ¥660
  • 圖片:
    • Western Blot
      Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate, HL60 whole cell lysate, MCF-7 whole cell lysate, LO2 whole cell lysate
      All lanes: HIST1H1C antibody at 1:500
      Secondary
      Goat polyclonal to rabbit IgG at 1/40000 dilution
      Predicted band size: 22 kDa
      Observed band size: 22 kDa
    • Chromatin Immunoprecipitation Hela (4*106) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 8µg anti-HIST1H1C (CSB-PA010378PA186me1HU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.
    • Immunofluorescence staining of MCF-7 cells with CSB-PA010378PA186me1HU at 1:5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品名稱:
    Rabbit anti-Homo sapiens (Human) HIST1H1C Polyclonal antibody
  • Uniprot No.:
  • 基因名:
  • 別名:
    H1 histone family member 2 antibody; H1.a antibody; H12_HUMAN antibody; H1F2 antibody; H1s-1 antibody; HIST1H1C antibody; Histone 1 H1c antibody; Histone cluster 1 H1c antibody; Histone H1.2 antibody; Histone H1c antibody; Histone H1d antibody; Histone H1s-1 antibody; MGC3992 antibody
  • 宿主:
    Rabbit
  • 反應(yīng)種屬:
    Human
  • 免疫原:
    Peptide sequence around site of Mono-methyl-Lys (186) derived from Human Histone H1.2
  • 免疫原種屬:
    Homo sapiens (Human)
  • 標(biāo)記方式:
    Non-conjugated
  • 克隆類型:
    Polyclonal
  • 抗體亞型:
    IgG
  • 純化方式:
    Antigen Affinity Purified
  • 濃度:
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液:
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 產(chǎn)品提供形式:
    Liquid
  • 應(yīng)用范圍:
    ELISA, WB, IF, ChIP
  • 推薦稀釋比:
    Application Recommended Dilution
    WB 1:500-1:2000
    IF 1:1-1:10
  • Protocols:
  • 儲(chǔ)存條件:
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期:
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途:
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價(jià)

靶點(diǎn)詳情

  • 功能:
    Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation.
  • 基因功能參考文獻(xiàn):
    1. results define a network of E2F target genes as susceptible to the regulatory influence of H1.2, where H1.2 augments global association of pRb with chromatin, enhances transcriptional repression by pRb, and facilitates pRb-dependent cell-cycle arrest PMID: 28614707
    2. BRG1 participates in gene repression by interacting with H1.2, facilitating its deposition and stabilizing nucleosome positioning around the transcription start site. PMID: 27390128
    3. Results show that histones H1.2 and H1.4 were observed in MDA-MB-231 metastatic breast cancer cells. The phosphorylation at S173 of histone H1.2 and S172, S187, T18, T146, and T154 of H1.4 significantly increases during M phase suggesting that these events are cell cycle-dependent. Also, the study reports the observation of the H1.2 SNP variant A18V in MCF-10A cells. PMID: 26209608
    4. Integration with apoptotic intermediates (via C-terminal tail interactions) may constitute a more generalized function of linker histone isoforms in apoptotic cascades. PMID: 24525734
    5. Histone H1.2-T165 post translational modifications are dispensable for chromatin binding and cell proliferation while the H1.4-K26 modifications are essential for proper cell cycle progression. PMID: 24873882
    6. H1.2 interacts with Cul4A and PAF1 to activate developmental regulatory genes. PMID: 24360965
    7. H1.2 is less abundant than other histone H1 variants at the transcription start sites of inactive genes, and promoters enriched in H1.2 are different from those enriched in other histone H1 variants and tend to be repressed. PMID: 24476918
    8. Mutations in linker histone genes HIST1H1 B, C, D, and E; OCT2 (POU2F2); IRF8; and ARID1A underlying the pathogenesis of follicular lymphoma. PMID: 24435047
    9. These data suggest that p53 acetylation-H1.2 phosphorylation cascade serves as a unique mechanism for triggering p53-dependent DNA damage response pathways. PMID: 22249259
    10. confirmed N-terminal acetylation on all isoforms plus a single internal acetylation site; phosphorylation sites were located on peptides containing the cyclin dependent kinase (CDK) consensus motif PMID: 15595731
    11. The binding of histone H1 to a general amyloid-like motif indicates that histone H1 may play an important common role in diseases associated with amyloid-like fibrils. PMID: 16854430
    12. Histone H1.2 was translocated from the nucleus to the mitochondria after treatment with bleomycin and co-localized with Bak in mitochondria. PMID: 17879944
    13. that the recruitment of YB1, PURalpha, and H1.2 to the p53 target gene Bax is required for repression of p53-induced transcription. PMID: 18258596

    顯示更多

    收起更多

  • 亞細(xì)胞定位:
    Nucleus. Chromosome. Note=Mainly localizes in euchromatin. Distribution goes in parallel with DNA concentration.
  • 蛋白家族:
    Histone H1/H5 family
  • 數(shù)據(jù)庫鏈接:

    HGNC: 4716

    OMIM: 142710

    KEGG: hsa:3006

    STRING: 9606.ENSP00000339566

    UniGene: Hs.7644



主站蜘蛛池模板: 中文字幕乱码久久午夜不卡| 欧美成人片在线观看| 狠狠躁夜夜躁人人爽天天不卡| 国产成人免费97在线观看| 色偷偷亚洲精品一区二区| 中文字幕乱码熟妇五十中出色欲 | 久久亚洲精品人成综合网| 亚洲精品国产高清一线久久| 亚洲日韩一区二区三区| 国产一区二区| 亚洲另类无码专区丝袜| 亚洲综合电影小说图片区| 免费又黄又爽又猛的毛片| 免费无码又爽又刺激软件下载直播 | 中文字幕久久熟女人妻av免费| 久久久这里只有免费精品| 国产人成网线在线播放va| 精产国品一二三产品麻豆| 北条麻妃在线一区二区| 国产三级精品三级在专区| 精品蜜臀av在线天堂| 97国产在线看片免费人成视频| 亚洲 a v无 码免 费 成 人 a v | 美丽的熟妇中文字幕| 亚洲综合色丁香婷婷六月图片| 扒开双腿猛进入喷水高潮叫声| 8x国产精品视频| 中文字幕日产无码| 玩弄人妻少妇500系列网址| 中文www天堂| 国产av午夜精品一区二区三| 国产成人精品久久亚洲高清不卡| 国产精品爱啪在线播放| 日本高清不卡aⅴ免费网站| 国产精品成人3p一区二区三区| 丰满人妻熟妇乱又伦精品软件 | 五月综合色婷婷在线观看| 欧美亚洲日本国产其他| 国产乱色国产精品播放视频| 久久精品无码一区二区无码| 影音先锋无码a∨男人资源站|