在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

Mono-methyl-HIST1H1C (K186) Antibody

  • 中文名稱:
    Mono-methyl-HIST1H1C (K186)兔多克隆抗體
  • 貨號:
    CSB-PA010378PA186me1HU
  • 規格:
    ¥660
  • 圖片:
    • Western Blot
      Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate, HL60 whole cell lysate, MCF-7 whole cell lysate, LO2 whole cell lysate
      All lanes: HIST1H1C antibody at 1:500
      Secondary
      Goat polyclonal to rabbit IgG at 1/40000 dilution
      Predicted band size: 22 kDa
      Observed band size: 22 kDa
    • Chromatin Immunoprecipitation Hela (4*106) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 8µg anti-HIST1H1C (CSB-PA010378PA186me1HU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.
    • Immunofluorescence staining of MCF-7 cells with CSB-PA010378PA186me1HU at 1:5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他:

產品詳情

  • 產品名稱:
    Rabbit anti-Homo sapiens (Human) HIST1H1C Polyclonal antibody
  • Uniprot No.:
  • 基因名:
  • 別名:
    H1 histone family member 2 antibody; H1.a antibody; H12_HUMAN antibody; H1F2 antibody; H1s-1 antibody; HIST1H1C antibody; Histone 1 H1c antibody; Histone cluster 1 H1c antibody; Histone H1.2 antibody; Histone H1c antibody; Histone H1d antibody; Histone H1s-1 antibody; MGC3992 antibody
  • 宿主:
    Rabbit
  • 反應種屬:
    Human
  • 免疫原:
    Peptide sequence around site of Mono-methyl-Lys (186) derived from Human Histone H1.2
  • 免疫原種屬:
    Homo sapiens (Human)
  • 標記方式:
    Non-conjugated
  • 克隆類型:
    Polyclonal
  • 抗體亞型:
    IgG
  • 純化方式:
    Antigen Affinity Purified
  • 濃度:
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液:
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 產品提供形式:
    Liquid
  • 應用范圍:
    ELISA, WB, IF, ChIP
  • 推薦稀釋比:
    Application Recommended Dilution
    WB 1:500-1:2000
    IF 1:1-1:10
  • Protocols:
  • 儲存條件:
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期:
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途:
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能:
    Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation.
  • 基因功能參考文獻:
    1. results define a network of E2F target genes as susceptible to the regulatory influence of H1.2, where H1.2 augments global association of pRb with chromatin, enhances transcriptional repression by pRb, and facilitates pRb-dependent cell-cycle arrest PMID: 28614707
    2. BRG1 participates in gene repression by interacting with H1.2, facilitating its deposition and stabilizing nucleosome positioning around the transcription start site. PMID: 27390128
    3. Results show that histones H1.2 and H1.4 were observed in MDA-MB-231 metastatic breast cancer cells. The phosphorylation at S173 of histone H1.2 and S172, S187, T18, T146, and T154 of H1.4 significantly increases during M phase suggesting that these events are cell cycle-dependent. Also, the study reports the observation of the H1.2 SNP variant A18V in MCF-10A cells. PMID: 26209608
    4. Integration with apoptotic intermediates (via C-terminal tail interactions) may constitute a more generalized function of linker histone isoforms in apoptotic cascades. PMID: 24525734
    5. Histone H1.2-T165 post translational modifications are dispensable for chromatin binding and cell proliferation while the H1.4-K26 modifications are essential for proper cell cycle progression. PMID: 24873882
    6. H1.2 interacts with Cul4A and PAF1 to activate developmental regulatory genes. PMID: 24360965
    7. H1.2 is less abundant than other histone H1 variants at the transcription start sites of inactive genes, and promoters enriched in H1.2 are different from those enriched in other histone H1 variants and tend to be repressed. PMID: 24476918
    8. Mutations in linker histone genes HIST1H1 B, C, D, and E; OCT2 (POU2F2); IRF8; and ARID1A underlying the pathogenesis of follicular lymphoma. PMID: 24435047
    9. These data suggest that p53 acetylation-H1.2 phosphorylation cascade serves as a unique mechanism for triggering p53-dependent DNA damage response pathways. PMID: 22249259
    10. confirmed N-terminal acetylation on all isoforms plus a single internal acetylation site; phosphorylation sites were located on peptides containing the cyclin dependent kinase (CDK) consensus motif PMID: 15595731
    11. The binding of histone H1 to a general amyloid-like motif indicates that histone H1 may play an important common role in diseases associated with amyloid-like fibrils. PMID: 16854430
    12. Histone H1.2 was translocated from the nucleus to the mitochondria after treatment with bleomycin and co-localized with Bak in mitochondria. PMID: 17879944
    13. that the recruitment of YB1, PURalpha, and H1.2 to the p53 target gene Bax is required for repression of p53-induced transcription. PMID: 18258596

    顯示更多

    收起更多

  • 亞細胞定位:
    Nucleus. Chromosome. Note=Mainly localizes in euchromatin. Distribution goes in parallel with DNA concentration.
  • 蛋白家族:
    Histone H1/H5 family
  • 數據庫鏈接:

    HGNC: 4716

    OMIM: 142710

    KEGG: hsa:3006

    STRING: 9606.ENSP00000339566

    UniGene: Hs.7644



主站蜘蛛池模板: 国内精品伊人久久久久妇| 超清无码av最大网站| 人人色在线视频播放| 无码熟熟妇丰满人妻啪啪| 亚洲乱码卡一卡二卡新区仙踪木木| 色哟哟精品视频在线观看| 国产精品亚洲а∨天堂2021| 精品国产免费一区二区三区 | 成 人 在 线 免费观看| 精品一卡二卡三卡四卡网站| 在线播放国产麻豆va剧情| 中国无码人妻丰满熟妇啪啪软件| 久久成人麻豆午夜电影| 天天做天天爱天天综合网| 麻豆丰满少妇chinese| 十八禁无码免费网站| 欧美牲交a欧美牲交aⅴ一| √8天堂资源地址中文在线| 精品国内自产拍在线观看| 人妻精品动漫h无码中字| 色综合久久精品亚洲国产| 欧美极品video粗暴| 久久久久99精品成人品| 亚洲免费观看视频| 国产人妻精品久久久久野外| 中文中幕a在线| 无码av片在线观看免费| 成人精品v视频在线| 久久国产精品二国产精品| 无码av片av片av无码| 亚洲中字幕日产av片在线| 性饥渴的农村熟妇| 日韩精品无码一区二区三区| 亚洲色一色噜一噜噜噜| 亚洲 欧美 国产 综合免费| 亚洲国产av一区二区三区| 日日躁夜夜躁狠狠躁| 国产日韩在线视看高清视频手机| 亚洲精品久久久久avwww潮水| 亚洲欧美中文日韩在线v日本| 日韩精品一区二区三区影院|