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  4. HIST1H2BB (Ab-16) Antibody

HIST1H2BB (Ab-16) Antibody

  • 中文名稱：
    HIST1H2BB (Ab-16)兔多克隆抗體
  • 貨號：
    CSB-PA010402OA16nacHU
  • 規(guī)格：
    ￥660
  • 圖片：
    • Western Blot
      Positive WB detected in: Hela cell acid extracts, Jurkat cell acid extracts, Mouse brain tissue, Mouse liver tissue, Mouse kidney tissue
      All lanes: HIST1H2BB antibody at 2.5µg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 14 kDa
      Observed band size: 14 kDa
    • Immunofluorescent analysis of Hela cells using CSB-PA010402OA16nacHU at dilution of 1:100 and Cy3-congugated Goat Anti-Rabbit IgG
    • Chromatin Immunoprecipitation Hela (4*106) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 8µg anti-HIST1H2BB (CSB-PA010402OA16nacHU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品名稱：
    Rabbit anti-Homo sapiens (Human) HIST1H2BB Polyclonal antibody
  • Uniprot No.：
  • 基因名：
    HIST1H2BB
  • 別名：
    HIST1H2BB antibody; H2BFFHistone H2B type 1-B antibody; Histone H2B.1 antibody; Histone H2B.f antibody; H2B/f antibody
  • 宿主：
    Rabbit
  • 反應(yīng)種屬：
    Human, Mouse
  • 免疫原：
    Peptide sequence around site of Lys (16) derived from Human Histone H2B type 1-B
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標(biāo)記方式：
    Non-conjugated
  • 克隆類型：
    Polyclonal
  • 抗體亞型：
    IgG
  • 純化方式：
    Antigen Affinity Purified
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, WB, IF, ChIP
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:200-1:2000
    IF 1:50-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價

靶點詳情

  • 功能：
    Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
  • 基因功能參考文獻(xiàn)：
    1. Functional crosstalk between histone H2B ubiquitylation and H2A modifications and variants has been uncovered. PMID: 29643390
    2. RNF20 and H2Bub1 promotes chronic colonic inflammation and inflammation-associated colorectal cancer in mice and humans, partly by augmenting NF-kappaB activity and attenuating the antitumoral T cell response. PMID: 26854224
    3. RNF20-mediated H2B ubiquitination at DSBs plays a critical role in HRR through chromatin remodeling PMID: 21362548
  • 亞細(xì)胞定位：
    Nucleus. Chromosome.
  • 蛋白家族：
    Histone H2B family
  • 數(shù)據(jù)庫鏈接：

    HGNC: 4751

    OMIM: 602803

    KEGG: hsa:3018

    STRING: 9606.ENSP00000350580

    UniGene: Hs.553494



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