在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

  1. Home
  2. 抗體
  3. 重組抗體
  4. Acetyl-Histone H2B type 1-B (K20) Recombinant Monoclonal Antibody

Acetyl-Histone H2B type 1-B (K20) Recombinant Monoclonal Antibody

  • 中文名稱：
    乙酰-組蛋白 H2B type 1-B (K20)重組抗體
  • 貨號：
    CSB-RA010402A20acHU
  • 規格：
    ￥1320
  • 圖片：
    • Western Blot
      Positive WB detected in Hela whole cell lysate treated by 15mM sodium butyrate for 30min
      All lanes Acetyl-Histone H2B type 1-B(K20)antibody at 0.135μg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 15 KDa
      Observed band size: 15 KDa
    • Immunocytochemistry analysis of CSB-RA010402A20acHU diluted at 1:100 and staining in Hela cells performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of Hela cells(treated by 15mM sodium butyrate for 30min) with CSB-RA010402A20acHU at 1:84,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
    • Overlay histogram showing Hela cells stained with CSB-RA010402A20acHU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min.The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4℃.The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4℃. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他：

產品詳情

  • 產品描述：
    CSB-RA010402A20acHU是針對組蛋白H2B第20位賴氨酸乙?；揎棧ˋcetyl-Histone H2B type 1-B K20）開發的重組單克隆抗體。組蛋白H2B作為核小體核心成分，其K20位點的乙?；揎椩诒碛^遺傳調控中發揮重要作用，參與染色質結構重塑、轉錄激活及DNA損傷修復等生物學過程。本產品經過ELISA、Western Blot（WB）、免疫細胞化學（ICC）、免疫熒光（IF）及流式細胞術（FC）等多平臺驗證，在WB實驗中表現出高特異性，推薦使用1:5000-1:10000稀釋比例可清晰檢測目標條帶；ICC/IF應用推薦1:50-1:500及1:30-1:200稀釋范圍，適用于細胞定位分析，能精準顯示核內乙酰化修飾信號；FC檢測則支持細胞群體表觀遺傳狀態分析。該抗體適用于染色質動態研究、組蛋白翻譯后修飾機制探索，以及發育生物學、腫瘤微環境等領域中表觀遺傳調控網絡的科研工作，為探索基因表達調控、干細胞多能性維持及疾病相關信號通路提供可靠工具。
  • Uniprot No.：
  • 基因名：
    HIST1H2BB
  • 別名：
    HIST1H2BB antibody; H2BFFHistone H2B type 1-B antibody; Histone H2B.1 antibody; Histone H2B.f antibody; H2B/f antibody
  • 反應種屬：
    Human
  • 免疫原：
    A synthesized peptide
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    21F11
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
  • 產品提供形式：
    Liquid
  • 應用范圍：
    ELISA, WB, ICC, IF, FC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:5000-1:10000
    ICC 1:50-1:500
    IF 1:30-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能：
    Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
  • 基因功能參考文獻：
    1. Functional crosstalk between histone H2B ubiquitylation and H2A modifications and variants has been uncovered. PMID: 29643390
    2. RNF20 and H2Bub1 promotes chronic colonic inflammation and inflammation-associated colorectal cancer in mice and humans, partly by augmenting NF-kappaB activity and attenuating the antitumoral T cell response. PMID: 26854224
    3. RNF20-mediated H2B ubiquitination at DSBs plays a critical role in HRR through chromatin remodeling PMID: 21362548
  • 亞細胞定位：
    Nucleus. Chromosome.
  • 蛋白家族：
    Histone H2B family
  • 數據庫鏈接：

    HGNC: 4751

    OMIM: 602803

    KEGG: hsa:3018

    STRING: 9606.ENSP00000350580

    UniGene: Hs.553494



主站蜘蛛池模板： 蜜臀av色欲a片无码精品一区| 野花社区视频www官网| 亚洲不卡中文字幕无码| 久久久亚洲欧洲日产国码606| 亚洲国产欧美不卡在线观看| 69国产成人精品午夜福中文| 小荡货好紧好爽奶头大视频| 亚洲精品日韩一区二区电影| 在线播放免费人成毛片试看| 色哟哟最新在线观看入口| 免费无码毛片一区二区三区a片 | 伊人无码精品久久一区二区| 成年轻人网站色直接看| 人人妻人人澡人人爽欧美一区双| 国产成人精品三上悠亚| 婷婷色爱区综合五月激情| 日产a一a区二区www| 国产佗精品一区二区三区| 天天躁夜夜躁av天天爽| 久久久久久久极品内射| 少妇高潮太爽了在线视| 亚洲精品成人福利网站app| 性色欲网站人妻丰满中文久久不卡 | 久久久久久九九99精品| 久久综合给综合给久久| 丁香五月激情综合亚洲| 精品无人国产偷自产在线| 中文字幕av在线一二三区| 人妻avav中文系列久久| 精品国产一区二区av麻豆| 久久久久99精品成人片直播| 在线观看免费网页欧美成| 97色偷偷色噜噜男人的天堂| 久久偷看各类wc女厕嘘嘘| 中文字幕精品无码一区二区三区| 日本午夜看x费免| 亚洲 欧美 唯美 国产 伦 综合| 欧美激情第1页| 日本中文字幕人妻不卡dvd| 97人摸人人澡人人人超碰| 国产熟女一区二区三区四区五区|