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  4. HAO1 Antibody

HAO1 Antibody

  • 中文名稱：
    HAO1兔多克隆抗體
  • 貨號：
    CSB-PA891938LA01HU
  • 規格：
    ￥440
  • 圖片：
    • IHC image of CSB-PA891938LA01HU diluted at 1:300 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of HepG2 cells with CSB-PA891938LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

產品詳情

  • 產品名稱：
    Rabbit anti-Homo sapiens (Human) HAO1 Polyclonal antibody
  • Uniprot No.：
  • 基因名：
    HAO1
  • 別名：
    (S) 2 hydroxy acid oxidase antibody; Glycolate oxidase antibody; GOX antibody; GOX1 antibody; HAO1 antibody; HAOX1 antibody; HAOX1_HUMAN antibody; Hydroxyacid oxidase 1 antibody; MGC142225 antibody; MGC142227 antibody; OTTHUMP00000030231 antibody
  • 宿主：
    Rabbit
  • 反應種屬：
    Human
  • 免疫原：
    Recombinant Human Hydroxyacid oxidase 1 protein (161-273AA)
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated

    本頁面中的產品，HAO1 Antibody (CSB-PA891938LA01HU)，的標記方式是Non-conjugated。對于HAO1 Antibody，我們還提供其他標記。見下表：

    可提供標記
    標記方式 貨號 產品名稱 應用
    HRP CSB-PA891938LB01HU HAO1 Antibody, HRP conjugated ELISA
    FITC CSB-PA891938LC01HU HAO1 Antibody, FITC conjugated
    Biotin CSB-PA891938LD01HU HAO1 Antibody, Biotin conjugated ELISA
  • 克隆類型：
    Polyclonal
  • 抗體亞型：
    IgG
  • 純化方式：
    >95%, Protein G purified
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 產品提供形式：
    Liquid
  • 應用范圍：
    ELISA, IHC, IF
  • 推薦稀釋比：
    Application Recommended Dilution
    IHC 1:200-1:500
    IF 1:50-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

問答及客戶評論

■ 常見問題解答
Q:

關于HAO-1抗體。我注意到大多數人說免疫原是重組人羥基酸氧化酶1蛋白(161-273AA)，使用這部分蛋白有原因嗎？

A:
我們利用該片段制備抗原以制備兔多克隆抗體，因為根據我們對該蛋白的分析，HAO1的161-273氨基酸具有最佳的免疫原性。

靶點詳情

  • 功能：
    Has 2-hydroxyacid oxidase activity. Most active on the 2-carbon substrate glycolate, but is also active on 2-hydroxy fatty acids, with high activity towards 2-hydroxy palmitate and 2-hydroxy octanoate.
  • 基因功能參考文獻：
    1. The study presents the first spectroscopic evidence of the formation of an intermediate with absorbance features resembling those of a flavosemiquinone in the oxidative half-reaction of glycolate oxidase. PMID: 21141873
    2. The inhibitor heteroatoms interact with five active-site residues that have been implicated in catalysis in homologous flavodehydrogenases of L-2-hydroxy acids. In addition, the chlorophenyl substituent is surrounded by nonconserved hydrophobic residues. PMID: 20054120
    3. These inhibitions suggest that glycolate binds to the active site of the reduced enzyme, and that DCIP also has affinity for the oxidized enzyme. PMID: 17669354
    4. Active site and loop 4 movements within human glycolate oxidase. PMID: 18215067
  • 亞細胞定位：
    Peroxisome.
  • 蛋白家族：
    FMN-dependent alpha-hydroxy acid dehydrogenase family
  • 組織特異性：
    Liver.
  • 數據庫鏈接：

    HGNC: 4809

    OMIM: 605023

    KEGG: hsa:54363

    STRING: 9606.ENSP00000368066

    UniGene: Hs.193640



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