在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

  1. 首頁
  2. 試劑盒
  3. 酶聯免疫試劑盒
  4. Human Hydroxyacid oxidase 1(HAO1) ELISA kit

Human Hydroxyacid oxidase 1(HAO1) ELISA kit

  • 中文名稱：
    人羥基酸氧化酶1(HAO1)酶聯免疫試劑盒
  • 貨號：
    CSB-EL010127HU
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產品詳情

  • 產品描述：
    人羥基酸氧化酶1(HAO1)酶聯免疫試劑盒（CSB-EL010127HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的HAO1含量。HAO1，即羥基酸氧化酶1，是一種FMN依賴的α-羥基酸脫氫酶家族成員，主要在肝臟的過氧化物酶體中氧化甘醇酸生成甘醇酸內酯，同時產生過氧化氫。其表達在氧化應激期間可能下調，以防止過氧化氫在過氧化物酶體中過量生成，可能是一種對氧化損傷的響應機制。試劑盒檢測范圍為18.75 pg/mL-1200 pg/mL，適用于科研場景中HAO1的功能研究，如代謝性疾病模型構建、肝腎組織病理分析、藥物作用靶點篩選等方向，為探索代謝調控機制及疾病干預策略提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    (S) 2 hydroxy acid oxidase ELISA Kit; Glycolate oxidase ELISA Kit; GOX ELISA Kit; GOX1 ELISA Kit; HAO1 ELISA Kit; HAOX1 ELISA Kit; HAOX1_HUMAN ELISA Kit; Hydroxyacid oxidase 1 ELISA Kit; MGC142225 ELISA Kit; MGC142227 ELISA Kit; OTTHUMP00000030231 ELISA Kit
  • 縮寫：
    HAO1
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates, cell lysates
  • 檢測范圍：
    18.75 pg/mL-1200 pg/mL
  • 靈敏度：
    4.69 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Cardiovascular
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human HAO1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:5 Average % 98    
    Range % 93-112    
    1:10 Average % 107    
    Range % 103-109    
    1:20 Average % 93    
    Range % 90-97    
    1:40 Average % 88    
    Range % 86-94    
  • 回收率：
    The recovery of human HAO1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 103 99-106    
    EDTA plasma (n=4) 92 90-95    
                   
                   
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    pg/ml OD1 OD2 Average Corrected    
    1200 2.193 2.369 2.281 2.097    
    600 1.700 1.710 1.705 1.521    
    300 1.253 1.262 1.258 1.074    
    150 0.878 0.898 0.888 0.704    
    75 0.532 0.566 0.549 0.365    
    37.5 0.411 0.422 0.417 0.233    
    18.75 0.231 0.222 0.227 0.043    
    0 0.177 0.190 0.184 　    
  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    Has 2-hydroxyacid oxidase activity. Most active on the 2-carbon substrate glycolate, but is also active on 2-hydroxy fatty acids, with high activity towards 2-hydroxy palmitate and 2-hydroxy octanoate.
  • 基因功能參考文獻：
    1. The study presents the first spectroscopic evidence of the formation of an intermediate with absorbance features resembling those of a flavosemiquinone in the oxidative half-reaction of glycolate oxidase. PMID: 21141873
    2. The inhibitor heteroatoms interact with five active-site residues that have been implicated in catalysis in homologous flavodehydrogenases of L-2-hydroxy acids. In addition, the chlorophenyl substituent is surrounded by nonconserved hydrophobic residues. PMID: 20054120
    3. These inhibitions suggest that glycolate binds to the active site of the reduced enzyme, and that DCIP also has affinity for the oxidized enzyme. PMID: 17669354
    4. Active site and loop 4 movements within human glycolate oxidase. PMID: 18215067
  • 亞細胞定位：
    Peroxisome.
  • 蛋白家族：
    FMN-dependent alpha-hydroxy acid dehydrogenase family
  • 組織特異性：
    Liver.
  • 數據庫鏈接：

    HGNC: 4809

    OMIM: 605023

    KEGG: hsa:54363

    STRING: 9606.ENSP00000368066

    UniGene: Hs.193640



主站蜘蛛池模板： 久久无码人妻一区二区三区| 免费大片av手机看片不卡| 天天爽夜夜爽人人爽88| 无码乱码av天堂一区二区| 人妻少妇69式99偷拍| 忍着娇喘人妻被中出中文字幕| 天堂亚洲2017在线观看| 国产综合久久亚洲综合| 色婷婷综合激情综在线播放| 日日噜噜夜夜狠狠久久丁香五月| 蜜臀色欲av在线播放国产日韩| 国产产品亚洲产品| 人妻少妇精品久久久久久| 亚欧成a人无码精品va片| 精品国产av最大网站| 成人片无码免费播放| 男人的天堂2018无码| 男女无遮挡xx00动态图120秒| 极品少妇被啪到呻吟喷水| 人人爽人人模人人人爽人人爱| 无码动漫性爽xo视频在线观看| 久青草久青草视频在线观看| 丁香五月欧美成人| 免费大黄网站在线观| 成年奭片免费观看视频天天看| 区二区三区国产精华液区别大吗| 国产一卡2卡3卡4卡网站精品| 狠狠色综合网站久久久久久久高清| 天堂网www天堂在线资源| 亚洲国产日韩a在线播放| 日本真人做爰免费视频120秒| 中文字幕精品无码一区二区| 亚洲a∨精品永久无码| 国产无遮挡又黄又爽不要vip软件 免费的av网站手机版 | 亚洲人成电影免费观看在线看| 免费看一区二区三区四区| 国产成人精品综合久久久| 午夜福利在线永久视频| 99久久婷婷国产一区二区| 亚洲午夜无码久久| 99久久精品国产一区二区三区|