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  4. GTF2F1 Antibody

GTF2F1 Antibody

  • 中文名稱：
    GTF2F1兔多克隆抗體
  • 貨號：
    CSB-PA010004HA01HU
  • 規格：
    ￥440
  • 圖片：
    • Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA010004HA01HU at dilution of 1:100
    • Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA010004HA01HU at dilution of 1:100
  • 其他：

產品詳情

  • 產品名稱：
    Rabbit anti-Homo sapiens (Human) GTF2F1 Polyclonal antibody
  • Uniprot No.：
  • 基因名：
    GTF2F1
  • 別名：
    2810405L04Rik antibody; BTF4 antibody; C76800 antibody; General transcription factor IIF 74 kDa subunit antibody; General transcription factor IIF subunit 1 antibody; General transcription factor IIF; polypeptide 1; 74kDa antibody; Gtf2f1 antibody; MGC94148 antibody; OTTHUMP00000237859 antibody; RAP74 antibody; T2FA_HUMAN antibody; TF2F1 antibody; TFIIF antibody; TFIIF-alpha antibody; Transcription initiation factor IIF subunit alpha antibody; Transcription initiation factor RAP74 antibody
  • 宿主：
    Rabbit
  • 反應種屬：
    Human
  • 免疫原：
    Recombinant Human General transcription factor IIF subunit 1 protein (1-249AA)
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated

    本頁面中的產品，GTF2F1 Antibody (CSB-PA010004HA01HU)，的標記方式是Non-conjugated。對于GTF2F1 Antibody，我們還提供其他標記。見下表：

    可提供標記
    標記方式 貨號 產品名稱 應用
    HRP CSB-PA010004HB01HU GTF2F1 Antibody, HRP conjugated ELISA
    FITC CSB-PA010004HC01HU GTF2F1 Antibody, FITC conjugated
    Biotin CSB-PA010004HD01HU GTF2F1 Antibody, Biotin conjugated ELISA
  • 克隆類型：
    Polyclonal
  • 抗體亞型：
    IgG
  • 純化方式：
    >95%, Protein G purified
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 產品提供形式：
    Liquid
  • 應用范圍：
    ELISA, IHC
  • 推薦稀釋比：
    Application Recommended Dilution
    IHC 1:20-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能：
    TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation.
  • 基因功能參考文獻：
    1. An AR motif of the transactivation domain has been identified that contributes to transcriptional activity by recruiting the C-terminal domain of subunit 1 of the general transcription regulator TFIIF. PMID: 29225078
    2. These results suggest that Mediator structural shifts induced by activator binding help stably orient pol II prior to transcription initiation within the human mediator-RNA polymerase II-TFIIF assembly. PMID: 22343046
    3. Gdown1 competes with TFIIF for binding to the RPB1 and RPB5 subunits of Pol II, thereby inhibiting an essential function of TFIIF in preinitiation complex assembly. PMID: 22244332
    4. role of associated carboxyl-terminal domain phosphatase in dephosphorylating phosphoserines 2 and 5 of RNA polymerase II PMID: 12351650
    5. The alpha 1 helix of human RAP74 has an important role in the initiation and elongation of RNA chains PMID: 12354769
    6. The NMR solution structure of the C-terminal domain of RAP74 has been determined, and NMR methods have been used to map the binding sites of the C-terminus of CTD phosphatase/FCP1 on the RAP74 C-terminal fragment. PMID: 12578358
    7. cocrystal structure of the winged-helix domain of human RNA polymerase II-associating protein 74 bound to the alpha-helical C terminus of human FCP1 PMID: 12591941
    8. NMR structure of a complex containing this TFIIF subunit and the RNA polymerase II carboxyl-terminal domain phosphatase FCP1. PMID: 12732728
    9. Interaction of TFIIF subunit RAP74 with recombinant androgen receptor N-terminal activation domain AF1 leads to imposition of helical structure on the AF1 domain. PMID: 15023052
    10. TFIIF supports elongation and suppresses pausing by stabilizing the post-translocated elongation complex PMID: 15351637
    11. alpha1-Helix of RAP74 is important for supporting NTP-driven translocation by RNAP II. PMID: 15831464
    12. TFIIF and Rpb7 are involved in both early and late transcriptional stages PMID: 17848138
    13. Mutated hydrophobic residues in RAP74 C-terminal structure disrupt secondary structure elements, showing that binding of the androgen receptor N-terminal domain depends upon helix 3 in the winged-helix domain of the RAP74 C-terminal domain polypeptide. PMID: 18284209
    14. NMR and thermodynamic studies further elucidate the complex molecular mechanism by which TFIIF and FCP1 cooperate for RNAPII recycling. PMID: 19215094

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  • 亞細胞定位：
    Nucleus.
  • 蛋白家族：
    TFIIF alpha subunit family
  • 數據庫鏈接：

    HGNC: 4652

    OMIM: 189968

    KEGG: hsa:2962

    STRING: 9606.ENSP00000377969

    UniGene: Hs.68257



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