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  4. ELP3 Antibody

ELP3 Antibody

  • 中文名稱：
    ELP3兔多克隆抗體
  • 貨號：
    CSB-PA884499LA01HU
  • 規格：
    ￥440
  • 圖片：
    • Western Blot
      Positive WB detected in: Jurkat whole cell lysate, K562 whole cell lysate, 293 whole cell lysate
      All lanes: ELP3 antibody at 3.82µg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 63, 61, 50, 53 kDa
      Observed band size: 63 kDa
    • IHC image of CSB-PA884499LA01HU diluted at 1:400 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of Hela cells with CSB-PA884499LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Immunoprecipitating ELP3 in HEK293 whole cell lysate
      Lane 1: Rabbit control IgG instead of CSB-PA884499LA01HU in HEK293 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
      Lane 2: CSB-PA884499LA01HU (6µg) + HEK293 whole cell lysate (500µg)
      Lane 3: HEK293 whole cell lysate (20µg)
  • 其他：

產品詳情

  • 產品名稱：
    Rabbit anti-Homo sapiens (Human) ELP3 Polyclonal antibody
  • Uniprot No.：
  • 基因名：
    ELP3
  • 別名：
    DKFZp761L098 antibody; Elongation protein 3 homolog (S. cerevisiae) antibody; Elongation protein 3 homolog antibody; ELONGATION PROTEIN 3; S. CEREVISIAE; HOMOLOG OF antibody; elongator acetyltransferase complex subunit 3 antibody; Elongator complex protein 3 antibody; elp3 antibody; ELP3_HUMAN antibody; FLJ10422 antibody; hELP3 antibody; Hypothetical protein DKFZp761L098 antibody; Kat9 antibody
  • 宿主：
    Rabbit
  • 反應種屬：
    Human
  • 免疫原：
    Recombinant Human Elongator complex protein 3 protein (1-85AA)
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated

    本頁面中的產品，ELP3 Antibody (CSB-PA884499LA01HU)，的標記方式是Non-conjugated。對于ELP3 Antibody，我們還提供其他標記。見下表：

    可提供標記
    標記方式 貨號 產品名稱 應用
    HRP CSB-PA884499LB01HU ELP3 Antibody, HRP conjugated ELISA
    FITC CSB-PA884499LC01HU ELP3 Antibody, FITC conjugated
    Biotin CSB-PA884499LD01HU ELP3 Antibody, Biotin conjugated ELISA
  • 克隆類型：
    Polyclonal
  • 抗體亞型：
    IgG
  • 純化方式：
    >95%, Protein G purified
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 產品提供形式：
    Liquid
  • 應用范圍：
    ELISA, WB, IHC, IF, IP
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:200-1:500
    IF 1:50-1:200
    IP 1:200-1:2000
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能：
    Catalytic tRNA acetyltransferase subunit of the RNA polymerase II elongator complex, which is a component of the RNA polymerase II (Pol II) holoenzyme and is involved in transcriptional elongation. The elongator complex is required for multiple tRNA modifications, including mcm5U (5-methoxycarbonylmethyl uridine), mcm5s2U (5-methoxycarbonylmethyl-2-thiouridine), and ncm5U (5-carbamoylmethyl uridine). In the elongator complex, acts as a tRNA uridine(34) acetyltransferase by mediating formation of carboxymethyluridine in the wobble base at position 34 in tRNAs. May also act as a protein lysine acetyltransferase by mediating acetylation of target proteins; such activity is however unclear in vivo and recent evidences suggest that ELP3 primarily acts as a tRNA acetyltransferase. Involved in neurogenesis: regulates the migration and branching of projection neurons in the developing cerebral cortex, through a process depending on alpha-tubulin acetylation. Required for acetylation of GJA1 in the developing cerebral cortex.
  • 基因功能參考文獻：
    1. and CTU1/2, partner enzymes in U34 methoxycarbonylmethyl-2-thio tRNA modification, are up-regulated in human breast cancers and sustain metastasis. PMID: 27811057
    2. Promoter hypermethylation is an important mechanism of the transcriptional inactivation of ELP3 in invasive ductal breast carcinoma. PMID: 25148870
    3. Regulation of G6PD acetylation by SIRT2 and KAT9 modulates NADPH homeostasis and cell survival during oxidative stress. PMID: 24769394
    4. data suggest that hElp3 can regulate the transcription of HSP70 gene, and the HAT domain of hElp3 is essential for this function PMID: 22216241
    5. The results of this study uncover a novel role for Elp3 in the regulation of synaptic bouton expansion during neurogenesis that may be linked with a requirement for sleep. PMID: 20626565
    6. Elp3 promotes the acetylation of alpha-tubulin in microtubules in neurological disorders. PMID: 20036197
    7. We used hELP3 antisense oligonucl. to knock down hELP3 gene. The results showed that reduction of hELP3 mRNA and protein caused a suppression of HSP70-2 and histone H3 hypoacetylation. PMID: 17558451
    8. Allelic variants of ELP3 were associated with amyotrophic lateral sclerosis in three populations comprising 1483 people. PMID: 18996918
    9. ELP3 localises to mitochondria in HeLa cells, actin-like filaments, and actin-rich sites at the edges of spreading cells. This suggests that ELP3 and the ELONGATOR complex may play a role in mitochondrial function, actin organisation, and cell motility. PMID: 19429107

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  • 相關疾病：
    ELP3 genetic variations may be associated with an increased risk for neurodegeneration and motor neuron diseases.
  • 亞細胞定位：
    Cytoplasm. Nucleus.; [Isoform 1]: Nucleus.; [Isoform 2]: Cytoplasm. Nucleus.
  • 蛋白家族：
    ELP3 family
  • 組織特異性：
    Expressed in the cerebellum and spinal motor neurons.
  • 數據庫鏈接：

    HGNC: 20696

    OMIM: 612722

    KEGG: hsa:55140

    STRING: 9606.ENSP00000256398

    UniGene: Hs.491336



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