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DMGDH Antibody

  • 中文名稱：
    DMGDH兔多克隆抗體
  • 貨號：
    CSB-PA883415LA01HU
  • 規(guī)格：
    ￥440
  • 圖片：
    • Western Blot
      Positive WB detected in: Mouse liver tissue, Mouse kidney tissue
      All lanes: DMGDH antibody at 6.7μg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 97, 46 kDa
      Observed band size: 97 kDa
    • Immunofluorescence staining of HepG2 cells with CSB-PA883415LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • IHC image of CSB-PA883415LA01HU diluted at 1:300 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • IHC image of CSB-PA883415LA01HU diluted at 1:300 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品名稱：
    Rabbit anti-Homo sapiens (Human) DMGDH Polyclonal antibody
  • Uniprot No.：
  • 基因名：
    DMGDH
  • 別名：
    Dimethylglycine dehydrogenase antibody; Dimethylglycine dehydrogenase; mitochondrial antibody; Dmgdh antibody; M2GD_HUMAN antibody; ME2GLYDH antibody; mitochondrial antibody
  • 宿主：
    Rabbit
  • 反應(yīng)種屬：
    Human, Mouse
  • 免疫原：
    Recombinant Human Dimethylglycine dehydrogenase, mitochondrial protein (429-524AA)
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標(biāo)記方式：
    Non-conjugated

    本頁面中的產(chǎn)品，DMGDH Antibody (CSB-PA883415LA01HU)，的標(biāo)記方式是Non-conjugated。對于DMGDH Antibody，我們還提供其他標(biāo)記。見下表：

    可提供標(biāo)記
    標(biāo)記方式 貨號 產(chǎn)品名稱 應(yīng)用
    HRP CSB-PA883415LB01HU DMGDH Antibody, HRP conjugated ELISA
    FITC CSB-PA883415LC01HU DMGDH Antibody, FITC conjugated
    Biotin CSB-PA883415LD01HU DMGDH Antibody, Biotin conjugated ELISA
  • 克隆類型：
    Polyclonal
  • 抗體亞型：
    IgG
  • 純化方式：
    >95%, Protein G purified
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, WB, IHC, IF
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:200-1:500
    IF 1:50-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價(jià)

靶點(diǎn)詳情

  • 功能：
    Catalyzes the demethylation of N,N-dimethylglycine to sarcosine. Also has activity with sarcosine in vitro.
  • 基因功能參考文獻(xiàn)：
    1. In nonalcoholic fatty liver disease patient, the missense variant p.Ser646Pro (rs1805074) in DMGDH gene was significantly associated with disease severity and circulating levels of dimethylglycine. PMID: 27614103
    2. The structure-based analysis provided new insights into the kinetic properties of dimethylglycine dehydrogenase in particular with respect to oxygen reactivity. PMID: 27486859
    3. In agreement with previous studies, we show that the genetic variant rs921943 in DMGDH is significantly associated with selenium status in United Kingdom pregnant women. PMID: 26675765
    4. Genetic variation of DMGDH was associated with higher plasma insulin, increased insulin resistance and increased risk of incident diabetes. PMID: 25795213
    5. analysis of dimethylglycine dehydrogenase deficiency associated with pathogenic variant H109R PMID: 18937046

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  • 相關(guān)疾病：
    DMGDH deficiency (DMGDHD)
  • 亞細(xì)胞定位：
    Mitochondrion.
  • 蛋白家族：
    GcvT family
  • 數(shù)據(jù)庫鏈接：

    HGNC: 24475

    OMIM: 605849

    KEGG: hsa:29958

    STRING: 9606.ENSP00000255189

    UniGene: Hs.655653



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