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CHST3 Antibody

  • 中文名稱：
    CHST3兔多克隆抗體
  • 貨號：
    CSB-PA759175LA01HU
  • 規(guī)格：
    ￥440
  • 圖片：
    • IHC image of CSB-PA759175LA01HU diluted at 1:300 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of A549 cells with CSB-PA759175LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品名稱：
    Rabbit anti-Homo sapiens (Human) CHST3 Polyclonal antibody
  • Uniprot No.：
  • 基因名：
    CHST3
  • 別名：
    C6ST 1 antibody; C6ST antibody; C6ST-1 antibody; C6ST1 antibody; Carbohydrate (chondroitin 6) sulfotransferase 3 antibody; Carbohydrate sulfotransferase 3 antibody; Chondroitin 6 O sulfotransferase 1 antibody; Chondroitin 6 sulfotransferase antibody; Chondroitin 6-O-sulfotransferase 1 antibody; Chondroitin 6-sulfotransferase antibody; CHST 3 antibody; Chst3 antibody; CHST3_HUMAN antibody; Galactose/N acetylglucosamine/N acetylglucosamine 6 O sulfotransferase 0 antibody; Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 0 antibody; GST 0 antibody; GST-0 antibody; GST0 antibody
  • 宿主：
    Rabbit
  • 反應(yīng)種屬：
    Human
  • 免疫原：
    Recombinant Human Carbohydrate sulfotransferase 3 protein (316-427AA)
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標(biāo)記方式：
    Non-conjugated

    本頁面中的產(chǎn)品，CHST3 Antibody (CSB-PA759175LA01HU)，的標(biāo)記方式是Non-conjugated。對于CHST3 Antibody，我們還提供其他標(biāo)記。見下表：

    可提供標(biāo)記
    標(biāo)記方式 貨號 產(chǎn)品名稱 應(yīng)用
    HRP CSB-PA759175LB01HU CHST3 Antibody, HRP conjugated ELISA
    FITC CSB-PA759175LC01HU CHST3 Antibody, FITC conjugated
    Biotin CSB-PA759175LD01HU CHST3 Antibody, Biotin conjugated ELISA
  • 克隆類型：
    Polyclonal
  • 抗體亞型：
    IgG
  • 純化方式：
    >95%, Protein G purified
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, IHC, IF
  • 推薦稀釋比：
    Application Recommended Dilution
    IHC 1:200-1:500
    IF 1:50-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價(jià)

靶點(diǎn)詳情

  • 功能：
    Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of the N-acetylgalactosamine (GalNAc) residue of chondroitin. Chondroitin sulfate constitutes the predominant proteoglycan present in cartilage and is distributed on the surfaces of many cells and extracellular matrices. Catalyzes with a lower efficiency the sulfation of Gal residues of keratan sulfate, another glycosaminoglycan. Can also catalyze the sulfation of the Gal residues in sialyl N-acetyllactosamine (sialyl LacNAc) oligosaccharides. May play a role in the maintenance of naive T-lymphocytes in the spleen.
  • 基因功能參考文獻(xiàn)：
    1. We describe three consanguineous Indian families with a distinct form of spondyloepiphyseal dysplasia (SED Omani type). It is an autosomal recessive disorder due to mutation in CHST3 gene. PMID: 27753269
    2. A novel CHST3 allele associated with spondyloepiphyseal dysplasia and hearing loss has been described in a consanguineous Pakistani pedigree. PMID: 26572954
    3. Sequencing of CHST3 detected a previously unreported homozygous duplication c.407_426dup (p.Thr143Cysfs*80). The mutation is predicted to lead to frameshift and introduction of a premature stopcodon. PMID: 24300290
    4. expression of CHST3 mRNA was significantly reduced in the intervertebral disc cells of subjects carrying the A allele of rs4148941 PMID: 24216480
    5. The results of this study indicated that the critical period for cortical plasticity is regulated by the 4S/6S ratio of CSPGs, which determines the maturation of parvalbumin-expressing interneurons. PMID: 22246436
    6. Vertebral changes, normal carpal age, lack of facial flattening, and recessive inheritance are characteristic and distinguishes CHST3 deficiency from other disorders with congenital dislocations. PMID: 20830804
    7. report eight CHST3 mutations in six unrelated individuals who presented at birth with congenital joint dislocations PMID: 18513679
    8. Sulfation of the galactose residues in the glycosaminoglycan-protein linkage region by recombinant human chondroitin 6-O-sulfotransferase-1. PMID: 18697746
    9. homozygous missense mutation (T141M) in exon 3 in all three family members with spondyloepiphyseal dysplasia with cardiac involvement PMID: 19320654

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  • 相關(guān)疾病：
    Spondyloepiphyseal dysplasia with congenital joint dislocations (SEDCJD)
  • 亞細(xì)胞定位：
    Golgi apparatus membrane; Single-pass type II membrane protein.
  • 蛋白家族：
    Sulfotransferase 1 family, Gal/GlcNAc/GalNAc subfamily
  • 組織特異性：
    Widely expressed in adult tissues. Expressed in heart, placenta, skeletal muscle and pancreas. Also expressed in various immune tissues such as spleen, lymph node, thymus and appendix.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 1971

    OMIM: 143095

    KEGG: hsa:9469

    STRING: 9606.ENSP00000362207

    UniGene: Hs.158304



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