Recombinant Human Tetraspanin-8 (TSPAN8)-VLPs (Active)

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中文名稱：

人TSPAN8-VLPs重組蛋白
貨號：

CSB-MP025166HU
規(guī)格：

￥5250
圖片：
CSB-MP025166HU is detected by Mouse anti-6*His monoclonal antibody.(This tag can be tested only under denaturing conditions.)

Activity
Measured by its binding ability in a functional ELISA. Immobilized Human TSPAN8 at 5 μg/ml can bind Anti-TSPAN8 recombinant antibody (CSB-RA025166MA1HU). The EC₅₀ is 2.261-2.623 ng/mL.The VLPs (CSB-MP3838) is negative control. Biological Activity Assay
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產(chǎn)品詳情

生物活性：

Measured by its binding ability in a functional ELISA. Immobilized Human TSPAN8 at 5 μg/mL can bind Anti-TSPAN8 recombinant antibody (CSB-RA025166MA1HU). The EC50 is 2.261-2.623 ng/mL.The VLPs (CSB-MP3838) is negative control.
基因名：

TSPAN8
Uniprot No.：

P19075
別名：

Transmembrane 4 superfamily member 3; Tumor-associated antigen CO-029
種屬：

Homo sapiens (Human)
蛋白長度：

Full Length
來源：

Mammalian cell
分子量：

27.4 kDa
表達(dá)區(qū)域：

1-237aa
氨基酸序列

MAGVSACIKYSMFTFNFLFWLCGILILALAIWVRVSNDSQAIFGSEDVGSSSYVAVDILIAVGAIIMILGFLGCCGAIKESRCMLLLFFIGLLLILLLQVATGILGAVFKSKSDRIVNETLYENTKLLSATGESEKQFQEAIIVFQEEFKCCGLVNGAADWGNNFQHYPELCACLDKQRPCQSYNGKQVYKETCISFIKDFLAKNLIIVIGISFGLAVIEILGLVFSMVLYCQIGNK
蛋白標(biāo)簽：

C-terminal 10xHis-tagged(This tag can be tested only under denaturing conditions)
產(chǎn)品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項：

The VLPs are expressed from human 293 cells (HEK293).Mix the sample gently by repeatedly pipetting it up and down. Do not vortex.Repeated freezing and thawing is not recommended.Store the protein at -20℃/-80℃ upon receiving it, and ensure to avoid repeated freezing and thawing, otherwise, it will affect the protein activity. The immunization strategy should be optimized (antigen dose, regimen and adjuvant).
Datasheet & COA：

Please contact us to get it.

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基因功能參考文獻：
1. TSPAN8 mediated Wnt/beta-catenin pathway through binding to NOTCH2. The role of TSPAN8 in the drug resistance of gastric cancer. PMID: 29345284
2. these data point to a crucial role of Co-029 in the modulation of colon cancer cell motility which could be related to the protumoral effect reported for this tetraspanin. Among surface molecules able to mediate Co-029 function, E-cadherin, EGFR and CD44 appear as likely candidates. PMID: 28418857
3. Single nucleotide polymorphism in TSPAN8 gene is associated with pathogenesis of bipolar disorder. PMID: 28777493
4. Tetraspanin-8 has a role in promoting hepatocellular carcinoma metastasis by increasing ADAM12m expression PMID: 27270327
5. LCMR1 modulation was sufficient to positively regulate endogenous Tspan8 expression, with concomitant in vitro phenotypic changes such as loss of melanoma cell-matrix adherence and increase in invasion, and Tspan8 expression promoted tumourigenicity in vivo. PMID: 27375018
6. Data indicate a regulatory role for tetraspanin 8 (Tspan8) in melanoma progression by modulating cell-matrix interactions through beta1 integrin - integrin-linked kinase (ILK) axis and establish Tspan8 as a negative regulator of ILK activity. PMID: 28188308
7. TSPAN8 might be important for the orchestration of meprin beta at the cell surface with impact on certain proteolytic processes PMID: 27180358
8. Knocking down TSPAN8 in AEG-1-overexpressing human hepatocellular carcinoma (HCC) cells markedly inhibited invasion and migration without affecting proliferation. TSPAN8 knockdown profoundly abrogated AEG-1-induced primary tumor and intrahepatic metastasis in an orthopic xenograft model in athymic nude mice PMID: 27339400
9. Knockdown and knockout models revealed that CD151 and Tspan8 act as molecular facilitators in wound healing, angiogenesis and tumor progression. [review] PMID: 28408484
10. Results provide evidence that TSPAN8 may contribute to the pathogenesis of lung cancer by promoting cell viability and proliferation. PMID: 27996312
11. TSPAN8 is involved in tumor progression and is an independent prognostic classifier in patients with gastric cancer. PMID: 25711980
12. This novel nuclear localization of TM4SF3 depends on androgen-induced nuclear localization of androgen receptor (AR) in both androgen-dependent and androgen-independent prostate cancer cell lines PMID: 26649804
13. Our data suggest that TSPAN8-LEL may play an important role in mCRC cell invasion, and that the antibody we have developed could be a useful tool for inhibiting the invasion of TSPAN8-expressing mCRCs. PMID: 26562525
14. Based on nasopharyngeal TSPAN8 gene expression in readily available Nasopharyngeal aspirate samples , we can discriminate between severity of disease in Respiratory syncytial virus infected infants. PMID: 25261323
15. Tspn8 was over-expressed in multiple clinical malignant glioma tissues, and its expression level correlated with the grade of tumors. PMID: 25680464
16. CO-029 functions as a regulator of both cell-matrix and cell-cell adhesion. During colon cancer progression, CO-029 promotes cancer cell movement by deregulating cell adhesions. PMID: 22679508
17. PMA-induced Tspan8-internalization promotes cell migration, but reduces matrix and cell adhesion. PMID: 20937409
18. High TSPAN8 is associated with melanoma invasiveness. PMID: 21081927
19. claudin-7-associated EpCAM is recruited into (tetraspanin-enriched membrane microdomains) and forms a complex with CO-029 and CD44v6 that facilitates metastasis formation PMID: 17579117
20. overexpression of TM4SF3 in esophageal cancer conferred advantage to the invasion and metastasis of this destructive disease PMID: 18365756
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亞細(xì)胞定位：

Membrane; Multi-pass membrane protein.
蛋白家族：

Tetraspanin (TM4SF) family
組織特異性：

Gastric, colon, rectal, and pancreatic carcinomas.
數(shù)據(jù)庫鏈接：

HGNC: 11855

OMIM: 600769

KEGG: hsa:7103

STRING: 9606.ENSP00000247829

UniGene: Hs.170563