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  4. Rat Tripartite motif-containing protein 72(TRIM72) ELISA kit

Rat Tripartite motif-containing protein 72(TRIM72) ELISA kit

  • 中文名稱：
    大鼠三結(jié)構(gòu)域包含蛋白72(TRIM72)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL024511RA
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠三結(jié)構(gòu)域包含蛋白72(TRIM72)酶聯(lián)免疫試劑盒（CSB-EL024511RA）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的TRIM72含量。TRIM72是重要靶點。它在細胞內(nèi)具有多種功能。研究發(fā)現(xiàn)其參與細胞膜修復等機制，在維持細胞正常生理功能方面發(fā)揮關(guān)鍵作用。對其深入研究有助于理解相關(guān)生理病理過程，為開發(fā)針對該靶點的治療策略提供理論支撐。試劑盒檢測范圍為18.75 pg/mL-1200 pg/mL，適用于研究TRIM72在心肌缺血再灌注損傷模型中的動態(tài)表達、骨骼肌再生機制探索或藥物干預后生物標志物水平變化的定量分析本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Trim72 ELISA Kit; Mg53 ELISA Kit; Tripartite motif-containing protein 72 ELISA Kit; Mitsugumin-53 ELISA Kit; Mg53 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates, cell lysates
  • 檢測范圍：
    18.75 pg/mL-1200 pg/mL
  • 靈敏度：
    4.7 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Cell Biology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat TRIM72 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %86
    Range %80-92
    1:2Average %97
    Range %91-105
    1:4Average %99
    Range %92-110
    1:8Average %93
    Range %86-98
  • 回收率：
    The recovery of rat TRIM72 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9489-98
    EDTA plasma (n=4)9690-100
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    12002.814 2.989 2.902 2.817
    6002.366 2.441 2.404 2.319
    3001.559 1.457 1.508 1.423
    1500.753 0.697 0.725 0.640
    750.469 0.442 0.456 0.371
    37.50.243 0.235 0.239 0.154
    18.750.149 0.139 0.144 0.059
    00.088 0.082 0.085 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Muscle-specific protein that plays a central role in cell membrane repair by nucleating the assembly of the repair machinery at injury sites. Specifically binds phosphatidylserine. Acts as a sensor of oxidation: upon membrane damage, entry of extracellular oxidative environment results in disulfide bond formation and homooligomerization at the injury site. This oligomerization acts as a nucleation site for recruitment of TRIM72-containing vesicles to the injury site, leading to membrane patch formation. Probably acts upstream of the Ca(2+)-dependent membrane resealing process. Required for transport of DYSF to sites of cell injury during repair patch formation. Regulates membrane budding and exocytosis. May be involved in the regulation of the mobility of KCNB1-containing endocytic vesicles.
  • 基因功能參考文獻：
    1. These results suggest that an 8-week swimming exercise improves HFD-induced insulin resistance maybe through a reduction of TRIM72 in skeletal muscle and enhancement of AKT signal transduction. PMID: 27843952
    2. MG53 is an effective biomarker of myocardial injury and dysfunction in murine hearts. However, MG53 is not expressed in human heart and therefore does not hold utility as a clinical biomarker of myocardial injury PMID: 26790476
    3. MG53 is a facilitator of rapid injury repair, a mediator of cell migration, and a modulator of myofibroblast differentiation during wound healing PMID: 26306047
    4. MG53, a member of the muscle-specific transmembrane adaptor molecule (TRIM) protein family, is a crucial component of the cardiac ischemic preconditioning response. PMID: 20516375
  • 亞細胞定位：
    Cell membrane, sarcolemma. Cytoplasmic vesicle membrane.
  • 蛋白家族：
    TRIM/RBCC family
  • 數(shù)據(jù)庫鏈接：


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