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中文名稱:大鼠前列腺素E2(PGE2)酶聯免疫試劑盒
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貨號:CSB-E07967r
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規格:96T/48T
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價格:¥3200/¥2500
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其他:
產品詳情
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產品描述:大鼠前列腺素E2(PGE2)酶聯免疫試劑盒(CSB-E07967r)為競爭法ELISA試劑盒,定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的PG-E2含量。PG-E2即前列腺素E2,它在體內廣泛存在,參與多種生理和病理過程。在炎癥、免疫調節、腫瘤發生發展等方面扮演重要角色。研究機制主要圍繞其與受體結合,激活多條信號通路,影響細胞增殖、分化和炎癥因子釋放等過程。試劑盒檢測范圍為0.5 pg/mL-100 pg/mL,適用于體外實驗研究,例如探究炎癥模型中PGE2的動態變化、評估藥物或化合物對前列腺素代謝通路的影響,以及組織微環境中脂質介質的定量分析等科研場景,為炎癥機制研究、藥效學評價及生物標志物探索提供高效檢測工具。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
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縮寫:PG-E2
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種屬:Rattus norvegicus (Rat)
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樣本類型:serum, plasma, tissue homogenates, cell lysates
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檢測范圍:0.5 pg/mL-100 pg/mL
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靈敏度:0.25 pg/mL
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反應時間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領域:Signal Transduction
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測定原理:quantitative
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測定方法:Competitive
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精密度:
Intra-assay Precision (Precision within an assay): CV%<15% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<15% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of rat PG-E2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 96 Range % 91-99 1:2 Average % 92 Range % 87-96 1:4 Average % 93 Range % 89-98 1:8 Average % 105 Range % 99-109 -
回收率:
The recovery of rat PG-E2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 96 92-99 EDTA plasma (n=4) 101 96-104 -
標準曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml OD1 OD2 Average 100 0.228 0.224 0.226 25 0.365 0.348 0.357 8 0.560 0.539 0.550 2 0.813 0.787 0.800 0.5 1.258 1.234 1.246 0 2.031 2.176 2.104 -
數據處理:
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貨期:3-5 working days
引用文獻
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產品評價
樣品類型:血清
樣品信息:大鼠
稀釋比:沒有稀釋
產品評價: 檢測pg-e2在血清中的含量,該試劑盒操作較方便,已經備好六只標準品,自行配液誤差較小,從實驗結果可以看出效果較好,希望其他試劑盒也能配好標準品,盡量減少操作可能出現誤差,簡化流程
By 李老師
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靶點詳情
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最新研究進展:PGE2是一種前列腺素,與炎癥和癌癥等多種疾病有關。近期的研究表明,PGE2可以在體內誘導肝癌干細胞分化為肝癌細胞,從而促進肝癌的發生和發展。此外,研究人員還發現,通過靶向PGE2的合成酶和受體可以減少腫瘤的生長和轉移,從而為腫瘤治療提供新的策略。
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