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中文名稱:犬前列腺素E2(PGE2)酶聯免疫試劑盒
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貨號:CSB-E13493c
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規格:96T/48T
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價格:¥3600/¥2500
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其他:
產品詳情
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產品描述:犬前列腺素E2(PGE2)酶聯免疫試劑盒(CSB-E13493c)為競爭法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的PG-E2含量。PG-E2即前列腺素E2,在人體生理與病理過程中廣泛參與。它參與炎癥反應、免疫調節等,還與腫瘤微環境形成相關。研究機制上,主要通過與細胞表面受體結合,激活信號通路,影響細胞增殖、凋亡等過程,是疾病治療研究的潛在靶點。試劑盒檢測范圍為10 pg/mL-2000 pg/mL,該產品特別適用于研究犬類炎癥性疾病(如關節炎、胃腸炎)、免疫應答機制、藥物抗炎效果評估,以及生殖系統或腫瘤相關研究中的PGE2動態監測本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
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縮寫:PG-E2
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種屬:Canis lupus familiaris (Dog) (Canis familiaris)
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樣本類型:serum, plasma, tissue homogenates
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檢測范圍:10 pg/mL-2000 pg/mL
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靈敏度:5 pg/mL
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反應時間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領域:Signal Transduction
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測定原理:quantitative
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測定方法:Competitive
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精密度:
Intra-assay Precision (Precision within an assay): CV%<15% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<15% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of canine PG-E2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 98 Range % 90-104 1:2 Average % 95 Range % 89-105 1:4 Average % 93 Range % 87-100 1:8 Average % 95 Range % 90-101 -
回收率:
The recovery of canine PG-E2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 95 85-96 EDTA plasma (n=4) 96 89-103 -
標準曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml OD1 OD2 Average 2000 0.187 0.174 0.181 500 0.320 0.315 0.318 160 0.517 0.547 0.532 40 0.814 0.848 0.831 10 1.316 1.374 1.345 0 1.958 1.933 1.946 -
數據處理:
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貨期:3-5 working days
引用文獻
- Preconditioning of canine adipose tissue-derived mesenchymal stem cells with deferoxamine potentiates anti-inflammatory effects by directing/reprogramming M2 macrophage polarization Park S M, et al,Veterinary Immunology and Immunopathology,2019
- Canine mesenchymal stem cells treated with TNF-α and IFN-r enhance anti-inflammatory effects through the COX-2/PGE2 pathway Hye-MiYang.et al,Research in Veterinary Science,2018
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