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Rat Matrix Metalloproteinase 14,MMP-14 ELISA Kit

  • 中文名稱:
    大鼠基質金屬蛋白酶14(MMP-14)酶聯(lián)免疫試劑盒
  • 貨號:
    CSB-E13158r
  • 規(guī)格:
    96T/48T
  • 價格:
    ¥3800/¥2500
  • 其他:

產品詳情

  • 產品描述:
    大鼠基質金屬蛋白酶14(MMP-14)酶聯(lián)免疫試劑盒(CSB-E13158r)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的MMP14含量。MMP14 即基質金屬蛋白酶 14,在細胞外基質重塑等生理過程中發(fā)揮重要作用。其異常表達與腫瘤生長、侵襲和轉移密切相關。研究聚焦于其催化機制和調控網絡,旨在揭示其在疾病發(fā)生發(fā)展中的作用,為開發(fā)針對性治療策略提供依據。試劑盒檢測范圍為46.88 pg/mL-3000 pg/mL,適用于探索大鼠模型中MMP-14在疾病發(fā)生發(fā)展中的作用機制,例如評估腫瘤微環(huán)境調控、組織修復異常或病理狀態(tài)下的酶活性變化。可為體外實驗提供定量數(shù)據支持,助力分子機制研究與藥物開發(fā)。本品僅用于科研,不用于臨床診斷,產品具體參數(shù)及操作步驟詳見產品說明書。
  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Rattus norvegicus (Rat)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    46.88 pg/mL-3000 pg/mL
  • 靈敏度:
    11.75 pg/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Cancer
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rat MMP-14 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
      Sample Serum(n=4)    
    1:1 Average % 91    
    Range % 88-95    
    1:2 Average % 95    
    Range % 90-102    
    1:4 Average % 97    
    Range % 95-107    
    1:8 Average % 101    
    Range % 96-105    
  • 回收率:
    The recovery of rat MMP-14 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 90 87-96    
    EDTA plasma (n=4) 98 95-103    
                   
                   
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    pg/ml OD1 OD2 Average Corrected    
    3000 2.288 2.289 2.289 2.092    
    1500 1.736 1.752 1.744 1.547    
    750 1.118 1.097 1.108 0.911    
    375 0.674 0.727 0.701 0.504    
    187.5 0.455 0.433 0.444 0.247    
    93.75 0.336 0.331 0.334 0.137    
    46.88 0.265 0.256 0.261 0.064    
    0 0.205 0.189 0.197      
  • 數(shù)據處理:
  • 貨期:
    3-5 working days

產品評價

靶點詳情

  • 功能:
    Endopeptidase that degrades various components of the extracellular matrix such as collagen. Activates progelatinase A. Essential for pericellular collagenolysis and modeling of skeletal and extraskeletal connective tissues during development. May be involved in actin cytoskeleton reorganization by cleaving PTK7. Acts as a positive regulator of cell growth and migration via activation of MMP15 in association with pro-MMP2. Involved in the formation of the fibrovascular tissues in association with pro-MMP2. Cleaves ADGRB1 to release vasculostatin-40 which inhibits angiogenesis.
  • 亞細胞定位:
    Membrane; Single-pass type I membrane protein. Melanosome. Cytoplasm.
  • 蛋白家族:
    Peptidase M10A family
  • 數(shù)據庫鏈接:


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