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Rabbit tissue inhibitors of metalloproteinase 1,TIMP-1 ELISA Kit

  • 中文名稱:
    兔子基質金屬蛋白酶抑制因子1(TIMP-1)酶聯免疫試劑盒
  • 貨號:
    CSB-E06935Rb
  • 規格:
    96T/48T
  • 價格:
    ¥4200/¥3000
  • 其他:

產品詳情

  • 產品描述:
    兔子基質金屬蛋白酶抑制因子1(TIMP-1)酶聯免疫試劑盒(CSB-E06935Rb)為競爭法ELISA試劑盒,定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的TIMP1含量。TIMP1即金屬蛋白酶組織抑制因子1,在細胞外基質代謝、腫瘤生長轉移等過程中扮演重要角色。研究機制方面,它可抑制基質金屬蛋白酶活性,調節細胞增殖、凋亡等。當前圍繞它在疾病診斷、治療靶點等方向開展諸多研究,有望為相關疾病干預提供新思路。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL,可滿足體外實驗中對TIMP - 1表達水平的動態監測需求,例如在細胞模型或動物實驗中探究炎癥反應、組織重塑或疾病進展相關機制,尤其適用于復雜生物樣本中TIMP - 1的科研級定量研究。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    TIMP1 ELISA Kit; Metalloproteinase inhibitor 1 ELISA Kit; Tissue inhibitor of metalloproteinases 1 ELISA Kit; TIMP-1 ELISA Kit
  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Oryctolagus cuniculus (Rabbit)
  • 樣本類型:
    serum, plasma, cell culture supernates , tissue homogenates
  • 檢測范圍:
    15.6 pg/mL-1000 pg/mL
  • 靈敏度:
    7.8 pg/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Cardiovascular
  • 測定原理:
    quantitative
  • 測定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rabbit TIMP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:100 Average % 90  
    Range % 85-97  
    1:200 Average % 102  
    Range % 98-108  
    1:400 Average % 98  
    Range % 94-102  
    1:800 Average % 88  
    Range % 84-93  
  • 回收率:
    The recovery of rabbit TIMP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 89-99  
    EDTA plasma (n=4) 92 88-96  
                 
                 
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    1000 0.086 0.084 0.085    
    500 0.145 0.141 0.143    
    250 0.245 0.238 0.242    
    125 0.392 0.413 0.403    
    62.5 0.725 0.727 0.726    
    31.2 0.963 0.944 0.954    
    15.6 1.154 1.179 1.167    
    0 1.734 1.662 1.698    
  • 數據處理:
  • 貨期:
    3-5 working days

產品評價

靶點詳情

  • 功能:
    Metalloproteinase inhibitor that functions by forming one to one complexes with target metalloproteinases, such as collagenases, and irreversibly inactivates them by binding to their catalytic zinc cofactor. Acts on MMP1, MMP2, MMP3, MMP7, MMP8, MMP9, MMP10, MMP11, MMP12, MMP13 and MMP16. Does not act on MMP14. Also functions as a growth factor that regulates cell differentiation, migration and cell death and activates cellular signaling cascades via CD63 and ITGB1. Plays a role in integrin signaling.
  • 基因功能參考文獻:
    1. Hemoperfusion could obviously reduce oxidative stress and the expression levels of MMP-2, MMP-9 and TIMP-1 in rabbits with acute paraquat poisoning. PMID: 22801080
  • 亞細胞定位:
    Secreted.
  • 蛋白家族:
    Protease inhibitor I35 (TIMP) family
  • 數據庫鏈接:


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