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Pig Heat Shock Protein 70,HSP-70 ELISA Kit

  • 中文名稱:
    豬熱休克蛋白70(HSP-70)酶聯免疫試劑盒
  • 貨號:
    CSB-E08317p
  • 規格:
    96T/48T
  • 價格:
    ¥4200/¥3000
  • 其他:

產品詳情

  • 產品描述:
    豬熱休克蛋白70(HSP-70)酶聯免疫試劑盒(CSB-E08317p)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的HSP70含量。HSP70 是重要的分子伴侶,在細胞應激反應中起關鍵作用,可保護細胞免受損害。研究表明,它與腫瘤發生、發展及神經退行性疾病相關。其作用機制主要是協助蛋白質折疊、轉運和降解,維持細胞內蛋白穩態,如今是藥物研發的熱門靶點。試劑盒檢測范圍為0.625 ng/mL-40 ng/mL,該產品適用于畜牧科研領域,可用于探究環境脅迫對豬生理狀態的影響、疾病模型中的應激響應機制,或作為評估豬群健康狀態的分子標志物,為動物營養調控、抗逆育種及病理學研究提供數據支持。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    HSPA1A ELISA Kit; HSPA1Heat shock 70 kDa protein 1A ELISA Kit; Heat shock 70 kDa protein 1 ELISA Kit; HSP70.1 ELISA Kit
  • 縮寫:
    HSP-70
  • Uniprot No.:
  • 種屬:
    Sus scrofa (Pig)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    0.625 ng/mL-40 ng/mL
  • 靈敏度:
    0.156 ng/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Signal Transduction
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of pig HSP-70 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 92  
    Range % 89-95  
    1:2 Average % 89  
    Range % 83-94  
    1:4 Average % 92  
    Range % 87-100  
    1:8 Average % 94  
    Range % 89-107  
  • 回收率:
    The recovery of pig HSP-70 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 94 87-99  
    EDTA plasma (n=4) 90 85-96  
                 
                 
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    40 1.952 1.931 1.942 1.807  
    20 1.570 1.613 1.592 1.457  
    10 1.235 1.268 1.252 1.117  
    5 0.911 0.897 0.904 0.769  
    2.5 0.672 0.656 0.664 0.529  
    1.25 0.409 0.395 0.402 0.267  
    0.625 0.265 0.251 0.258 0.123  
    0 0.138 0.131 0.135    
  • 數據處理:
  • 貨期:
    3-5 working days

引用文獻

產品評價

靶點詳情

  • 功能:
    Molecular chaperone implicated in a wide variety of cellular processes, including protection of the proteome from stress, folding and transport of newly synthesized polypeptides, activation of proteolysis of misfolded proteins and the formation and dissociation of protein complexes. Plays a pivotal role in the protein quality control system, ensuring the correct folding of proteins, the re-folding of misfolded proteins and controlling the targeting of proteins for subsequent degradation. This is achieved through cycles of ATP binding, ATP hydrolysis and ADP release, mediated by co-chaperones. The co-chaperones have been shown to not only regulate different steps of the ATPase cycle, but they also have an individual specificity such that one co-chaperone may promote folding of a substrate while another may promote degradation. The affinity for polypeptides is regulated by its nucleotide bound state. In the ATP-bound form, it has a low affinity for substrate proteins. However, upon hydrolysis of the ATP to ADP, it undergoes a conformational change that increases its affinity for substrate proteins. It goes through repeated cycles of ATP hydrolysis and nucleotide exchange, which permits cycles of substrate binding and release. The co-chaperones are of three types: J-domain co-chaperones such as HSP40s (stimulate ATPase hydrolysis by HSP70), the nucleotide exchange factors (NEF) such as BAG1/2/3 (facilitate conversion of HSP70 from the ADP-bound to the ATP-bound state thereby promoting substrate release), and the TPR domain chaperones such as HOPX and STUB1. Maintains protein homeostasis during cellular stress through two opposing mechanisms: protein refolding and degradation. Its acetylation/deacetylation state determines whether it functions in protein refolding or protein degradation by controlling the competitive binding of co-chaperones HOPX and STUB1. During the early stress response, the acetylated form binds to HOPX which assists in chaperone-mediated protein refolding, thereafter, it is deacetylated and binds to ubiquitin ligase STUB1 that promotes ubiquitin-mediated protein degradation. Regulates centrosome integrity during mitosis, and is required for the maintenance of a functional mitotic centrosome that supports the assembly of a bipolar mitotic spindle. Enhances STUB1-mediated SMAD3 ubiquitination and degradation and facilitates STUB1-mediated inhibition of TGF-beta signaling. Essential for STUB1-mediated ubiquitination and degradation of FOXP3 in regulatory T-cells (Treg) during inflammation. Negatively regulates heat shock-induced HSF1 transcriptional activity during the attenuation and recovery phase period of the heat shock response.
  • 亞細胞定位:
    Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Secreted.
  • 蛋白家族:
    Heat shock protein 70 family


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