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Your Good Partner in Biology Research

  • 中文名稱(chēng):
    豬D二聚體(D2D)酶聯(lián)免疫試劑盒
  • 貨號(hào):
    CSB-E09399p
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥4200/¥3000
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    豬D二聚體(D2D)酶聯(lián)免疫試劑盒(CSB-E09399p)為雙抗夾心法ELISA試劑盒,定量檢測(cè)血清、血漿、組織勻漿樣本中的D2D含量。試劑盒檢測(cè)范圍為12.5 ng/mL-800 ng/mL,常用于評(píng)估血栓性疾病、炎癥反應(yīng)或組織損傷后的病理進(jìn)程;適用于心血管疾病模型研究、術(shù)后凝血功能監(jiān)測(cè)、藥物療效評(píng)估等科研場(chǎng)景;為動(dòng)物疾病機(jī)制研究及生物標(biāo)志物探索提供高效技術(shù)支持。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 縮寫(xiě):
    D2D
  • 種屬:
    Sus scrofa (Pig)
  • 樣本類(lèi)型:
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍:
    12.5 ng/mL-800 ng/mL
  • 靈敏度:
    3.12 ng/mL
  • 反應(yīng)時(shí)間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測(cè)波長(zhǎng):
    450 nm
  • 研究領(lǐng)域:
    Cardiovascular
  • 測(cè)定原理:
    quantitative
  • 測(cè)定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線(xiàn)性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of pig D2D in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 92  
    Range % 80-99  
    1:2 Average % 92  
    Range % 85-100  
    1:4 Average % 90  
    Range % 84-99  
    1:8 Average % 96  
    Range % 91-102  
  • 回收率:
    The recovery of pig D2D spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 98 89-107  
    EDTA plasma (n=4) 95 90-100  
                 
                 
  • 標(biāo)準(zhǔn)曲線(xiàn):
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    800 2.894 2.915 2.905 2.769  
    400 2.117 2.135 2.126 1.990  
    200 1.255 1.263 1.259 1.123  
    100 0.827 0.850 0.839 0.703  
    50 0.487 0.491 0.489 0.353  
    25 0.316 0.337 0.327 0.191  
    12.5 0.252 0.241 0.247 0.111  
    0 0.135 0.137 0.136    
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

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