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中文名稱:小鼠D二聚體(D2D)酶聯免疫試劑盒
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貨號:CSB-E13584m
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規格:96T/48T
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價格:¥3800/¥2500
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其他:
產品詳情
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產品描述:小鼠D二聚體(D2D)酶聯免疫試劑盒(CSB-E13584m)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的D2D含量。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL,廣泛應用于心血管疾病模型、凝血功能障礙機制研究、藥物作用評估等基礎科研領域,尤其適合在小鼠模型中動態監測凝血系統活化程度或評價抗血栓藥物干預效果,為實驗室提供穩定可靠的檢測工具。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
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縮寫:D2D
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種屬:Mus musculus (Mouse)
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樣本類型:serum, plasma, cell culture supernates, tissue homogenates
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檢測范圍:31.25 pg/mL-2000 pg/mL
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靈敏度:7.81 pg/mL
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反應時間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領域:Cardiovascular
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測定原理:quantitative
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測定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of mouse D2D in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:100 Average % 91 Range % 86-98 1:200 Average % 97 Range % 92-105 1:400 Average % 93 Range % 87-98 1:800 Average % 95 Range % 87-103 -
回收率:
The recovery of mouse D2D spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 99 92-105 EDTA plasma (n=4) 97 92-102 -
標準曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml OD1 OD2 Average Corrected 2000 2.519 2.419 2.469 2.300 1000 1.801 1.701 1.751 1.582 500 1.122 1.082 1.102 0.933 250 0.674 0.664 0.669 0.500 125 0.438 0.428 0.433 0.264 62.5 0.355 0.345 0.350 0.181 31.25 0.289 0.284 0.287 0.118 0 0.170 0.168 0.169 -
數據處理:
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貨期:3-5 working days
引用文獻
- Interleukin-6 elevates thrombosis via pro-coagulant phospholipids from platelet 12-lipoxygenase in rheumatoid arthritis. D Costa, S Hughes, R Jenkins, A Cardus Figueras,bioRxiv,2025
- Multifunctional Co‐Delivery Systems with Downregulation of the Novel Target PIM1 in Macrophages to Ameliorate TF‐Mediated Coagulopathy in Sepsis A Zhou, J Cai, Y Wang, R Zhang, J Tan, C Zhou, S Luo,Small,2025
- Gut microbial co-metabolite 2-methylbutyrylcarnitine exacerbates thrombosis via binding to and activating integrin a2b1 K Huang, Z Li, X He, J Dai, B Huang, Y Shi, D Fan, Z Zhang, Y Liu, N Li, Z Zhang, J Peng,Cell Metabolism,2024
- Fibroblast growth factor 2 (FGF2) ameliorates the coagulation abnormalities in sepsis Y Sun,Toxicology and applied pharmacology,2023
- Piperlongumin Improves Survival in the Mouse Model of Sepsis: Effect on Coagulation Factors and Lung Inflammation Z Fang,Inflammation,2022
- Fibroblast growth factor-21 as a novel metabolic factor for regulating thrombotic homeostasis S Li,scientific reports,2022
- Bacteria-released outer membrane vesicles promote disseminated intravascular coagulation Wang E, et al,Thrombosis Research,2019
- Establishment and evaluation of COPD model by chronic exposure to MVE combined with LPS instillation Jiaze Shu.et al,experimental physiology,2018
- Comparison and evaluation of two different methods to establish the cigarette smoke exposure mouse model of COPD Shu J.et al,Sci Rep,2017
- Thrombin-Activatable Fibrinolysis Inhibitor Protects Against Acute Lung Injury by Inhibiting the Complement System Naito M et al,Am J Respir Cell Mol Biol,2013
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