Mouse D-Dimer,D2D ELISA Kit

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Mouse D-Dimer,D2D ELISA Kit

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文獻引用 (10) 說明書下載

中文名稱：

小鼠D二聚體(D2D)酶聯免疫試劑盒
貨號：

CSB-E13584m
規格：

96T/48T
價格：

￥3800/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

產品詳情
產品評價
引用文獻
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產品描述：

小鼠D二聚體(D2D)酶聯免疫試劑盒（CSB-E13584m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的D2D含量。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL，廣泛應用于心血管疾病模型、凝血功能障礙機制研究、藥物作用評估等基礎科研領域，尤其適合在小鼠模型中動態監測凝血系統活化程度或評價抗血栓藥物干預效果，為實驗室提供穩定可靠的檢測工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

D2D
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

31.25 pg/mL-2000 pg/mL
靈敏度：

7.81 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cardiovascular
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse D2D in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	91
	Range %	86-98
1:200	Average %	97
	Range %	92-105
1:400	Average %	93
	Range %	87-98
1:800	Average %	95
	Range %	87-103

回收率：

The recovery of mouse D2D spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	99	92-105
EDTA plasma (n=4)	97	92-102

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.519	2.419	2.469	2.300
1000	1.801	1.701	1.751	1.582
500	1.122	1.082	1.102	0.933
250	0.674	0.664	0.669	0.500
125	0.438	0.428	0.433	0.264
62.5	0.355	0.345	0.350	0.181
31.25	0.289	0.284	0.287	0.118
0	0.170	0.168	0.169

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Interleukin-6 elevates thrombosis via pro-coagulant phospholipids from platelet 12-lipoxygenase in rheumatoid arthritis. D Costa, S Hughes, R Jenkins, A Cardus Figueras,bioRxiv,2025
Multifunctional Co‐Delivery Systems with Downregulation of the Novel Target PIM1 in Macrophages to Ameliorate TF‐Mediated Coagulopathy in Sepsis A Zhou, J Cai, Y Wang, R Zhang, J Tan, C Zhou, S Luo,Small,2025
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Piperlongumin Improves Survival in the Mouse Model of Sepsis: Effect on Coagulation Factors and Lung Inflammation Z Fang,Inflammation,2022
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Establishment and evaluation of COPD model by chronic exposure to MVE combined with LPS instillation Jiaze Shu.et al,experimental physiology,2018
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Thrombin-Activatable Fibrinolysis Inhibitor Protects Against Acute Lung Injury by Inhibiting the Complement System Naito M et al,Am J Respir Cell Mol Biol,2013

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.