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Mouse Tri-iodothyronine,T3 ELISA Kit

  • 中文名稱:
    小鼠三碘甲狀腺原氨酸(T3)酶聯免疫試劑盒
  • 貨號:
    CSB-E05086m
  • 規格:
    96T/48T
  • 價格:
    ¥3800/¥2500
  • 其他:

產品詳情

  • 產品描述:
    小鼠三碘甲狀腺原氨酸(T3)酶聯免疫試劑盒(CSB-E05086m)為競爭法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的T3含量。試劑盒檢測范圍為0.5 ng/mL-8 ng/mL,靈敏度為0.5 ng/mL。科研人員可將其應用于甲狀腺功能研究、代謝性疾病動物模型評估、內分泌干擾物效應分析,以及藥物研發中甲狀腺激素調節劑的藥效評價等領域,尤其適合需要高通量檢測的實驗室進行批量樣本分析,為探索甲狀腺相關分子機制提供可靠的數據支持。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 縮寫:
    T3
  • 種屬:
    Mus musculus (Mouse)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    0.5 ng/mL-8 ng/mL
  • 靈敏度:
    0.5 ng/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Signal Transduction
  • 測定原理:
    quantitative
  • 測定方法:
    Competitive
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<15%

     

    Three samples of known concentration were tested twenty times on one plate to assess.

     

    Inter-assay Precision (Precision between assays): CV%<15%

     

    Three samples of known concentration were tested in twenty assays to assess.

      

  • 標準曲線:

     These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

  • 本試劑盒所含材料:
      • An assay plate --- The 96-well plate has been pre-coated with an anti-mouse T3 antibody.
      • Standard (6 x 1 ml) --- Reconstitute one of the standards with sample diluent at dilution series and draw the standard curve.
      • Biotin-conjugated T3 (1 x 6 ml)
      • HRP-avidin (1 x 6 ml)--- HRP catalyzes the TMB substrate for color development and needs to be diluted before use.
      • Wash Buffer (20 x concentrate) (1 x 15 ml) --- Wash away the unbound solution and non-specific substances.
      • Substrate A (1 x 7 ml) --- Mix with substrate B, and the TMB is catalyzed by HRP to elicit a chromogenic signal.
      • Substrate B (1 x 7 ml) --- Mix with substrate A, and the TMB is catalyzed by HRP to elicit a chromogenic signal.
      • Stop Solution (1 x 7 ml) --- Add stop solution and stop the color development. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strip (For 96 wells) --- Cover the microtiter plate when incubation.
      • An instruction manual

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  • 本試劑盒不含材料:
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm - 630 nm.
      • An incubator that can provide stable incubation conditions up to 37°C±5°C.
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer
      • Absorbent paper for blotting the microtiter plate
      • 50-300ul multi-channel micropipette
      • Pipette tips
      • Single-channel micropipette with different ranges
      • 100ml and 500ml graduated cylinders
      • Deionized or distilled water
      • Timer
      • Test tubes for dilution

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  • 數據處理:
  • 貨期:
    3-5 working days

引用文獻

產品評價

相關問答

 常見問題解答
Q:

Please let us know delivery time and the sulfuric acid content of [stop solution] .

A:
Thanks for your inquiry. The sulfuric acid content of [stop solution] is 4N. Delivery time is usually around 3-5 business days.

靶點詳情

  • 最新研究進展:
    T3是一種重要的甲狀腺激素,對于機體的發育、生長和代謝具有重要作用。最新的研究表明,T3可以通過多種信號通路調節細胞的增殖、分化和凋亡等生命活動,同時還能夠影響多個重要的代謝通路。


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