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Goat Tri-iodothyronine(T3) ELISA KIT

  • 中文名稱:
    山羊三碘甲狀腺原氨酸(T3)酶聯(lián)免疫試劑盒
  • 貨號:
    CSB-E13385G
  • 規(guī)格:
    96T/48T
  • 價格:
    ¥4200/¥3000
  • 其他:

產品詳情

  • 產品描述:
    山羊三碘甲狀腺原氨酸(T3)酶聯(lián)免疫試劑盒(CSB-E13385G)為競爭法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的T3含量。試劑盒檢測范圍為0.5 ng/mL-8 ng/mL,靈敏度為0.5 ng/mL。適用于科研領域如畜牧動物生理研究、飼料營養(yǎng)干預實驗、甲狀腺相關疾病機制探索及藥物代謝評估等方向,有助于研究山羊內分泌功能、代謝疾病模型或環(huán)境因素對甲狀腺軸的影響本品僅用于科研,不用于臨床診斷,產品具體參數(shù)及操作步驟詳見產品說明書。
  • 縮寫:
    T3
  • 種屬:
    Capra hircus (Goat)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    0.5 ng/mL-8 ng/mL
  • 靈敏度:
    0.5 ng/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Signal Transduction
  • 測定原理:
    quantitative
  • 測定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<15%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<15%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of goat T3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
      Sample Serum(n=4)    
    1:1 Average % 95    
    Range % 90-103    
    1:2 Average % 95    
    Range % 85-102    
    1:4 Average % 93    
    Range % 90-97    
    1:8 Average % 94    
    Range % 88-99    
  • 回收率:
    The recovery of goat T3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 97 86-102    
    EDTA plasma (n=4) 96 92-100    
                   
                   
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    ng/ml OD1 OD2 Average      
    8 0.166 0.168 0.167      
    4 0.281 0.288 0.285      
    2 0.558 0.553 0.556      
    1 1.262 1.247 1.255      
    0.5 2.105 2.138 2.122      
  • 數(shù)據處理:
  • 貨期:
    3-5 working days

產品評價

相關問答

 常見問題解答
Q:

I'm studying metabolic stress in dairy goats in the peripartum.
In total, 450 samples have to be analysed for each hormone, and analysis will be done in duplicate. Meaning I'll need a number of kits enough to run 900 tests.
Could you please send me a quote specifying the plate size (i.e. 96 well) and the number of free wells per plate. (i.e. 96 well minus the number of wells used for the standard curve).

A:
Thanks for your inquiry. We have the mentioned kit available.For example:
#CSB-E13385G,Goat Tri-iodothyronine(T3) ELISA KIT *96T (If duplicate done,22 kits is advised;If not,only 11 kits is ok)
the standard wells are S1-S5 and duplicate is advised.With a blank well,the number of standard wells is 5×2+1=11.The rest is for samples wells,85=96-11(If duplicate is done for sample,then the number will be the half of that.)
If you want to detect diary goat samples,we suggest you do pre-testing first.

靶點詳情

  • 最新研究進展:
    近期研究表明,T3在許多重要的生理過程中扮演著關鍵角色,如代謝調節(jié)、心臟功能、神經發(fā)育和免疫系統(tǒng)等。此外,一些研究還發(fā)現(xiàn),T3可能在腫瘤生長和轉移中發(fā)揮一定作用,值得深入探究。


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