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Mouse Nerve growth factor,NGF ELISA kit

  • 中文名稱:
    小鼠神經生長因子(NGF)酶聯免疫試劑盒
  • 貨號:
    CSB-E04684m
  • 規格:
    96T/48T
  • 價格:
    ¥3800/¥2500
  • 其他:

產品詳情

  • 產品描述:
    小鼠神經生長因子(NGF)酶聯免疫試劑盒(CSB-E04684m)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿.樣本中的NGF含量。NGF即神經生長因子,是神經營養因子家族一員,在神經元生長、發育、存活及維持正常功能中起關鍵作用。研究機制包括與特異性受體結合啟動細胞內信號傳導通路,調節基因表達等,對神經系統疾病治療有潛在意義。試劑盒檢測范圍為62.5 pg/ml-4000 pg/ml,適用于神經科學領域的多種研究場景,包括神經損傷動物模型評估、體外神經元培養體系中神經營養因子動態監測、神經退行性疾病機制探究以及藥物干預效果分析等。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    Ngf ELISA Kit; Ngfb ELISA Kit; Beta-nerve growth factor ELISA Kit; Beta-NGF ELISA Kit
  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Mus musculus (Mouse)
  • 樣本類型:
    serum, plasma, tissue homogenates.
  • 檢測范圍:
    62.5 pg/ml-4000 pg/ml
  • 靈敏度:
    15.6 pg/ml
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Neuroscience
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of mouse NGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

     

    Sample

    Serum(n=4)

    1:1

    Average %

    97

    Range %

    92-102

    1:2

    Average %

    91

    Range %

    85-98

    1:4

    Average %

    90

    Range %

    88-93

    1:8

    Average %

    100

    Range %

    96-104

  • 回收率:

    The recovery of mouse NGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    90

    87-92

    EDTA plasma (n=4)

    95

    91-98

  • 標準曲線:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml

    OD1

    OD2

    Average

    Corrected

    4000

    2.802

    2.752

    2.777

    2.610

    2000

    2.439

    2.376

    2.408

    2.241

    1000

    1.786

    1.662

    1.724

    1.557

    500

    1.018

    1.009

    1.014

    0.847

    250

    0.531

    0.528

    0.530

    0.363

    125

    0.388

    0.359

    0.374

    0.207

    62.5

    0.278

    0.289

    0.284

    0.117

    0

    0.168

    0.165

    0.167

     

  • 數據處理:
  • 貨期:
    3-5 working days

產品評價

靶點詳情

  • 功能:
    Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades to regulate neuronal proliferation, differentiation and survival. The immature NGF precursor (proNGF) functions as ligand for the heterodimeric receptor formed by SORCS2 and NGFR, and activates cellular signaling cascades that lead to inactivation of RAC1 and/or RAC2, reorganization of the actin cytoskeleton and neuronal growth cone collapse. In contrast to mature NGF, the precursor form (proNGF) promotes neuronal apoptosis (in vitro). Inhibits metalloproteinase-dependent proteolysis of platelet glycoprotein VI. Binds lysophosphatidylinositol and lysophosphatidylserine between the two chains of the homodimer. The lipid-bound form promotes histamine relase from mast cells, contrary to the lipid-free form.
  • 基因功能參考文獻:
    1. The authors described a long-distance signaling mechanism of Porcine hemagglutinating encephalomyelitis virus-driven deficits in neurons and suggested that such Ulk1 repression may result in limited NGF/TrkA retrograde signaling within activated Rab5 endosomes, explaining the progressive failure of neurite outgrowth and survival. PMID: 29875237
    2. Mechanoinsensitive ''silent'' nociceptors are characterized by the expression of the nicotinic acetylcholine receptor subunit alpha-3 (CHRNA3); the mechanically gated ion channel PIEZO2 mediates NGF-induced mechanosensitivity in these neurons. PMID: 29241539
    3. antibodies raised against NGF, TrkA, and p75 (also known as CD271) were used to explore the expression of these antigens in the non-decalcified young mouse femur. PMID: 29166838
    4. An inducible mouse model that can dissect the contribution of autocrine direct action of cleavage-resistant proNGF on systemic microvascular abnormalities in both retina and kidney, major targets for microvascular complication. PMID: 29253516
    5. both in vivo mechanical loading and in vitro mechanical stretch were shown to induce the profound up-regulation of NGF in osteoblasts within 1 h of loading. PMID: 28416686
    6. Nerve growth factor negatively regulates bone marrow granulopoiesis during small intestinal inflammation PMID: 26683342
    7. This study may represent a common mechanism for selective follicular activation induced by a localized increase in NGF in interstitial cells and mediated via the mTOR signaling pathway. PMID: 28542147
    8. nerve growth factor (NGF) signaling through neurotrophic tyrosine kinase receptor type 1 (TrkA) directs innervation of the developing mouse femur to promote vascularization and osteoprogenitor lineage progression. PMID: 27568565
    9. Mechanical stress-induced upregulation of NGF in colon SMC underlies the visceral hypersensitivity in bowel obstruction through increased gene expression and activity of tetrodotoxin-resistant Na channels in sensory neurons. PMID: 28079757
    10. results suggest that perivascular nerves innervate neovessels as neovasculatures mature and that NGF accelerates the innervation of perivascular nerves in neovessels. PMID: 27493098
    11. These findings reveal a non-neuronal role for neurotrophins and identify a new regulatory pathway in insulin secretion that can be targeted to ameliorate beta cell dysfunction. PMID: 27825441
    12. NGF signalling directly controls basal APP phosphorylation, subcellular localization and BACE cleavage. PMID: 27076121
    13. NGF facilitates OVA with lowLPS-induced maturation of mouse BMDCs through LPS-up-regulated p75 NTR via activation of NF-kappaB pathways, providing another mechanism for the involvement of NGF in the Th2 response PMID: 27437725
    14. NGF-OE mice exhibit age-dependent increases in Substance P and CGRP in the urothelium and hyperinnervation of the bladder. PMID: 27342083
    15. TNF-alpha upregulated Nerve Growth Factor [NGF] expression in synovial fibroblasts and macrophages and IL-1beta upregulated NGF expression in synovial fibroblasts. IL-1beta and TNF-alpha may regulate NGF signaling in Osteoarthritic joints and be suitable therapeutic targets for treating Osteoarthritis pain PMID: 27635406
    16. functional PAP(thorn) neurons are essential for the analgesic effect, which is mediated by NGF-trkA signaling. PMID: 27306411
    17. NGF negatively regulates growth cone retrograde actin flow on laminin. PMID: 26631553
    18. In E-Reeler retinas, NGF was significantly increased in retinal ganglion cells and glial cells. E-Reeler retinal bipolar cells and RGCs overexpress NGF and p75(NTR) as a protective endogenous response to Reelin deprivation. PMID: 26066836
    19. The NGF-induced up-regulation of TRPV1 via the increased synthesis and release of endogenous CGRP leads to improved cardiac performance in I/R-injured diabetic heart. PMID: 25650182
    20. NGF effects on parasympathetic nerves may regulate airway smooth muscle. PMID: 25647301
    21. Ginger extract has a synaptogenic effect via NGF-induced ERK/CREB activation, resulting in memory enhancement. PMID: 25049196
    22. Proinflammatory cytokines in osteoarthritis (OA) joints and the increased mechanical loading of cartilage may mediate OA pain via the stimulation of NGF expression and release by chondrocytes PMID: 24438745
    23. Despite being highly conserved, NGF and proNGF of mouse and human origins show distinct properties.Special care must be taken in performing experiments with cross-species systems. PMID: 25496838
    24. NGF exhibits anti-oxidative and hepatoprotective effects and is suggested to be therapeutically applicable in treating cholestatic liver diseases. PMID: 25397406
    25. Results indicate that analgesic effect of CB1 activation may in part be due to inhibition of NGF-induced sensitization of TRPV1 and also that the effect of CB1 activation is at least partly mediated by attenuation of NGF-induced increased PI3 signaling PMID: 25088915
    26. NGF induces removal of active caspase-3 in a lysosome-dependent manner. PMID: 24787014
    27. These results indicate that NGF exerts antileishmanial effect by stimulating hydrogen peroxide production in macrophages. PMID: 24937592
    28. iron accumulation induces NGF expression in hepatocytes, which in turn leads to LSEC defenestration via TrkA PMID: 25460199
    29. These findings suggest that overexpression of NGF in the ovary may suffice to cause both reproductive and metabolic alterations characteristic of polycystic ovary-like syndrome (PCOS) and support the hypothesis that sympathetic hyperactivity may contribute to the development and/or progression of PCOS. PMID: 25211588
    30. The increased NGF concentrations abolish Sema3A-induced inhibitory effect on axon outgrowth, while they have no effect on Sema3A-induced collapse rate. PMID: 24338202
    31. Data suggest that proNGF may appeal a new pathway or possible mechanism underlying microglial toxicity in the neuroinflammation and a potential target for therapeutic manipulation of the neurodegenerative diseases. PMID: 24040063
    32. beta-NGF gene transfection promotes the differentiation of bone marrow stromal stem cells into neurons through regulation of AKT and MAPKs signaling pathways. PMID: 23934089
    33. mechanical stimulation may attenuate NGFbeta signaling through Rac1 PMID: 23989259
    34. Independent of genotype, folate deficiency affects NGF levels in the frontal cortex, amygdala and hippocampus. PMID: 23623989
    35. calcineurin/NFAT pathway mediates the upregulation of PAI-1 by NGF PMID: 23825664
    36. ProNGF\NGF imbalance triggers learning and memory deficits, neurodegeneration and spontaneous epileptic-like discharges in transgenic mice. PMID: 23538417
    37. elevated levels of NGF in target tissues stimulate sympathetic and sensory axonal sprouting PMID: 23322532
    38. PIP5Kalpha acts as a negative regulator of nerve growth factor-induced neurite outgrowth by inhibiting PI3K/Akt signaling pathway in PC12 cells. PMID: 23538529
    39. Data suggest that diet factors (i.e., olive pomace polyphenols) up-regulate NGF/TrkA (proto-oncogene trk) and BDNF/TrkB (brain derived neurotrophic factor/receptor) in hippocampus/olfactory bulb and down-regulate NGF/BDNF in frontal cortex/striatum. PMID: 23466052
    40. Data suggest that expression of NGF is down-regulated in wounded skin (epidermis) in diabetes; among the growth factors investigated, only expression of NGF was down-regulated in healing skin wounds of diabetic mice as compared to nondiabetic mice. PMID: 23426701
    41. proNGF selectively promotes the growth of neurites from a subset of NGF-responsive neurons by a p75(NTR)-dependent mechanism during postnatal development when the axons of these neurons are ramifying within their targets in vivo PMID: 23633509
    42. Overexpression of mouse NGF in urothelium & detrusor affected transcription of PAC1, VPAC1, VPAC2, PAPCAP, VIP & other peptides normally & in cyclophosphamide-induced cystitis. The changes were tissue- and disease-duration dependent. PMID: 22700375
    43. The HTM1 heterodimer of 2 NGF muteins binds p75 and TrkA on opposite sides of the heterodimer, but not 2 TrkA receptors, supporting the ligand passing of NGF from p75 to TrkA via a transient heteroreceptor complex of p75-NGF-TrkA. PMID: 22903500
    44. Our results suggest that BDNF-TrkB but not NGF-TrkA signaling is involved in the brain repair after ICH, and early proper treadmill exercise might promote this repair process. PMID: 22999926
    45. Expression of NGF in hippocampus, cortex, and adrenal gland of wild type animal tended to decrease following spaceflight. PMID: 22808101
    46. This study demonstrated that both TrkA and NGF support the survival of only a subset of basal forebrain cholinergic neuron during brain development. PMID: 23100411
    47. Letter: NGF-p75 and neuropsin/KLK8 pathways may cooperate in regulation of epidermal homeostasis in inflamed skin. PMID: 22520925
    48. that NGF derived from bronchial and alveolar epithelium plays an important role in airway hyperresponsiveness after chronic exposure to mite antigen PMID: 22168511
    49. Therapeutic potential of NGF for the prevention of cardiomyopathy in diabetic subjects. PMID: 22187379
    50. NGF exerts profibrotic activities in the airways by inducing type III collagen production in fibroblasts PMID: 21816457

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  • 亞細胞定位:
    Secreted. Endosome lumen.
  • 蛋白家族:
    NGF-beta family
  • 組織特異性:
    Detected in submaxillary gland (at protein level). Highly expressed in male submaxillary gland. Levels are much lower in female submaxillary gland.
  • 數據庫鏈接:

    KEGG: mmu:18049

    STRING: 10090.ENSMUSP00000102538

    UniGene: Mm.1259



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