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Mouse Growth/differentiation factor 15(GDF15) ELISA kit

  • 中文名稱:
    小鼠生長分化因子15(GDF15)酶聯免疫試劑盒
  • 貨號:
    CSB-EL009345MO
  • 規格:
    96T/48T
  • 價格:
    ¥3600/¥2500
  • 其他:

產品詳情

  • 產品描述:
    小鼠生長分化因子15(GDF15)酶聯免疫試劑盒(CSB-EL009345MO)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的GDF15含量。GDF15屬于TGF-β超家族成員,在細胞應激時表達上調。其作為應激反應細胞因子,能調節能量代謝等生理過程。研究聚焦于它在代謝性疾?。ㄈ绶逝?、糖尿病)及癌癥等領域的作用機制,有望為相關疾病診療提供新思路。試劑盒檢測范圍為7.8 pg/ml-500 pg/ml,支持科研人員探究GDF15在代謝綜合征、肥胖相關疾病、心血管異常及腫瘤微環境調控等領域的分子機制,為動物模型研究提供可靠工具;適用于實驗室常規檢測本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    Gdf15 ELISA Kit; Mic1 ELISA Kit; SbfGrowth/differentiation factor 15 ELISA Kit; GDF-15 ELISA Kit; Macrophage inhibitory cytokine 1 ELISA Kit; MIC-1 ELISA Kit
  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Mus musculus (Mouse)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    7.8 pg/ml -500 pg/ml
  • 靈敏度:
    1.95 pg/ml
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Signal Transduction
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of mouse GDF15 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

     

    Sample

    Serum(n=4)

    1:1

    Average %

    92

    Range %

    88-96

    1:2

    Average %

    95

    Range %

    91-99

    1:4

    Average %

    92

    Range %

    87-98

    1:8

    Average %

    93

    Range %

    88-96

  • 回收率:

    The recovery of mouse GDF15 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    94

    90-98

    EDTA plasma (n=4)

    91

    86-98

  • 標準曲線:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml

    OD1

    OD2

    Average

    Corrected

    500

    2.254

    2.111

    2.183

    2.020

    250

    1.636

    1.694

    1.665

    1.502

    125

    1.022

    1.112

    1.067

    0.904

    62.5

    0.575

    0.603

    0.589

    0.426

    31.2

    0.334

    0.337

    0.336

    0.173

    15.6

    0.228

    0.237

    0.233

    0.070

    7.8

    0.201

    0.215

    0.208

    0.045

    0

    0.160

    0.166

    0.163

     

  • 數據處理:
  • 貨期:
    3-5 working days

引用文獻

產品評價

靶點詳情

  • 功能:
    Regulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses. Binds to its receptor, GFRAL, and activates GFRAL-expressing neurons localized in the area postrema and nucleus tractus solitarius of the brainstem. It then triggers the activation of neurons localized within the parabrachial nucleus and central amygdala, which constitutes part of the 'emergency circuit' that shapes feeding responses to stressful conditions. On hepatocytes, inhibits growth hormone signaling.
  • 基因功能參考文獻:
    1. GDF15 is a myokine secreted by aging-related stress and may control aging phenotype PMID: 29491220
    2. Membrane-protein extraction and use of an intracellular protein-transport inhibitor showed that GDF-15 promoted CaV3.1 and CaV3.3 alpha-subunit expression by trafficking to the membrane. PMID: 27353765
    3. CHOP activates Gdf15 transcription via binding to its promoter upon endoplasmic reticulum stress. PMID: 28847729
    4. The ANT1-deficient muscle mitochondria produce excess reactive oxygen species (ROS) and are partially uncoupled. Hence, the muscle respiration under nonphosphorylating conditions is increased. Muscle transcriptome analysis revealed the induction of mitochondrial biogenesis, down-regulation of diabetes-related genes, and increased expression of the genes encoding the myokines FGF21 and GDF15. PMID: 28223503
    5. isolating GFRAL as the receptor for GDF15-induced anorexia and weight loss, the identification of a mechanistic basis for the non-homeostatic regulation of neural circuitry by a peripheral signal associated with tissue damage and stress; findings provide opportunities to develop therapeutic agents for the treatment of disorders with altered energy demand PMID: 28953886
    6. These data suggest that GDF15 is required for IL-13-induced improvement of glucose intolerance in mice fed an HFD. PMID: 28874416
    7. HIF-1alpha and GDF15 expression are inversely related under normoxia and hyperoxia. PMID: 28734801
    8. This is the first study defining the role of Mic-1/Gdf15 in a number of behavioural domains. PMID: 28081177
    9. epithelial restitution was promoted by enhanced NAG-1 expression and subsequent enterocyte locomotion during the early wound-healing process PMID: 27421482
    10. GDF15 is a potent mitohormetic signal that safeguards against the onset of obesity and insulin resistance. PMID: 27986797
    11. The aim of this study was to identify the role of endogenous Gdf-15 in the MPTP (1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine) mouse model of Parkinson's disease (PD) by comparing Gdf-15 (+/+) and Gdf-15 (-/-) mice. PMID: 27115420
    12. The results of this study indicated the importance of Gdf-15 in promoting survival of DAergic neurons and regulating the inflammatory response post 6-OHDA lesion. PMID: 26733415
    13. endogenous Growth/differentiation factor-15 is beneficial for axon regeneration following sciatic nerve crush PMID: 26077927
    14. repeated cisterna magna administration of human umbilical cord blood-derived mesenchymal stem cells enhanced endogenous adult hippocampal neurogenesis and synaptic activity through a paracrine factor of GDF-15 PMID: 26154268
    15. Levels of GDF-15 mRNA increased 2 h after induction of ventilator-induced lung injury in WT lungs. Non-hematopoietic cells are the main source of GDF-15. GDF-15 is required for platelet-leukocyte interactions during ventilator-induced lung injury. PMID: 25948320
    16. hNAG-1 appears to be a novel therapeutic target in preventing and treating obesity and insulin resistance. PMID: 24531647
    17. Collectively, hNAG-1 is an important regulator of mammalian longevity and may act as a survival factor. PMID: 25239873
    18. Counterbalance between MIC-1 and ATF3 is critical for deciding the fate of enterocytes under the food chemical stress. PMID: 25180886
    19. Growth differentiation factor 15, a marker of lung involvement in systemic sclerosis, is involved in fibrosis development but is not indispensable for fibrosis development. PMID: 24504814
    20. Growth/differentiation factor 15 promotes EGFR signalling, and regulates proliferation and migration in the hippocampus of neonatal and young adult mice. PMID: 24496615
    21. GDF-15 is therefore prominently upregulated in the retina after optic nerve crush but does not directly interfere with the magnitude and temporal progression of retinal ganglion cell death. PMID: 23640134
    22. GDF15 protects the renal interstitium and tubular compartment in experimental type 1 and 2 diabetes without affecting glomerular damage PMID: 23986522
    23. Enteropathogenic Escherichia coli enhances MIC-1 gene expression in the human intestinal cancer cells PMID: 23503457
    24. knockdown of MIC-1 can decrease RNPC1-induced cell growth suppression. PMID: 23836903
    25. This is the first study that demonstrates a protective role of GDF-15 in advanced atherosclerosis and macrophage accumulation, possibly due to the reduced expression of adhesion molecules. PMID: 23800095
    26. MIC-1/GDF15 is involved in the physiological regulation of appetite and energy storage. PMID: 23468844
    27. GDF-15 specifically inhibits platelet integrin activation, preventing thrombus formation. PMID: 23231375
    28. GDF-15 is involved in orchestrating atherosclerotic lesion progression by regulating apoptotic cell death and IL-6-dependent inflammatory responses to vascular injury. PMID: 23316317
    29. Increased expression of the TGF-b superfamily cytokine MIC-1/GDF15 protects ApoE(-/-) mice from the development of atherosclerosis. PMID: 22386250
    30. GDF15 impairs aortic contractile and relaxing function through an endothelium-dependent mechanism involving altered caveolar endothelial NO synthase signaling. PMID: 23262134
    31. MIC-1/GDF15 has complex actions on tumor behavior PMID: 22952779
    32. identified the secreted protein follistatin-like 1 (FSTL1) as an inducer of GDF15 production. PMID: 22675198
    33. The work shows that the molecule MIC-1/GDF15 might be beneficial for the treatment of obesity as well as perturbations in glucose homeostasis. PMID: 22514681
    34. These findings suggest that GDF15 could play a pivotal role in the pathogenesis of bone loss relevant to hypoxia. PMID: 22190281
    35. coronary artery ligation in Gdf15-deficient mice led to enhanced recruitment of polymorphonuclear leukocytes into the infarcted myocardium and an increased incidence of cardiac rupture PMID: 21516086
    36. Regulation of GDF-15, a distant TGF-beta superfamily member, in a mouse model of cerebral ischemia. PMID: 21128084
    37. GDF-15 deletion has a beneficial effect both in early and later atherosclerosis by inhibition of CCR2-mediated chemotaxis and by modulating cell death. PMID: 21242297
    38. GDF-15 at endogenous levels contributes to proliferation and immune escape of malignant gliomas in an immunocompetent host. PMID: 20534737
    39. Data show that MIC-1 is secreted from melanoma cells together with VEGF to promote vascular development mediated by (V600E)B-Raf signaling. PMID: 20431030
    40. GDF-15 prevents apoptosis in cerebellar granule neurons by activating Akt and inhibiting endogenously active ERK PMID: 12514175
    41. GDF-15/MIC-1 is an early mediator of the injury response in kidney and lung PMID: 15897808
    42. GDF-15 induction is an immediate early response to liver injury that can occur through TNF and p53 independent pathways PMID: 16154591
    43. GDF15 is a novel autocrine/endocrine factor that antagonizes the hypertrophic response and loss of ventricular performance. PMID: 16397142
    44. gdf15 is a growth factor with dual function either promoting proliferation or growth arrest and differentiation due most likely to differences in cellular differentiation PMID: 17286605
    45. GDF-15 has no apparent effect on hepatocellular carcinoma (HCC) tumor formation rate, growth rate or invasiveness in diethylnitrosamine-induced HCC in vivo. PMID: 18210153
    46. Data conclude that MIC-1 is expressed in adipose tissue and secreted from adipocytes and is therefore a new adipokine. MIC-1 may have a paracrine role in the modulation of adipose tissue function and body fat mass. PMID: 19074584
    47. a possible inverse relationship exists between the expression of NAG-1 and COX-2 in tumor formation of colon tissue. PMID: 19375854
    48. the NAG-1 protein inhibits urethane-induced tumor formation PMID: 19401523
    49. Despite striking similarities in the GDF-15 and CNTF knock-out phenotypes, expression levels of CNTF and other neurotrophic factors in the sciatic nerve were unaltered suggesting that GDF-15 is a genuine novel trophic factor for motor and sensory neurons. PMID: 19864576

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  • 亞細胞定位:
    Secreted.
  • 蛋白家族:
    TGF-beta family
  • 組織特異性:
    Highly expressed in liver. Detected in plasma (at protein level). Expressed by cardiomyocytes, expression is highly increased in heart diseases. Also detected in subcutaneous fat.
  • 數據庫鏈接:


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