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  4. Mouse Glycated hemoglobin A1c,GHbA1c ELISA Kit

Mouse Glycated hemoglobin A1c,GHbA1c ELISA Kit

  • 中文名稱：
    小鼠糖化血紅蛋白A1c(GHbA1c)酶聯免疫試劑盒
  • 貨號：
    CSB-E08141m
  • 規格：
    96T/48T
  • 價格：
    ￥3800/￥2500
  • 其他：

產品詳情

  • 產品描述：
    小鼠糖化血紅蛋白A1c(GHbA1c)酶聯免疫試劑盒（CSB-E08141m）為競爭法ELISA試劑盒，定量檢測lysatesforRBC樣本中的GHbA1c含量。GHbA1c即糖化血紅蛋白，反映過去 2 至 3 個月的平均血糖水平。它是評估糖尿病長期血糖控制的重要指標。研究機制上，其由血紅蛋白與葡萄糖非酶促結合而成，結合量與血糖濃度正相關，可更全面反映血糖波動情況。試劑盒檢測范圍為62.5 ng/ml-4000 ng/ml.，適用于科研實驗中紅細胞裂解液樣本的定量分析；常用于糖尿病相關機制研究及代謝性疾病模型的評估；適用于體外實驗中對紅細胞樣本的糖化程度分析，可應用于糖尿病動物模型研究、降糖藥物作用機制探索或慢性高血糖對紅細胞功能影響的科研項目中本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 縮寫：
    GHbA1c
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    lysates for RBC
  • 檢測范圍：
    62.5 ng/ml-4000 ng/ml.
  • 靈敏度：
    15.6ng/ml
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Others
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of mouse GHbA1c in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Lysate for RBC(n=4)

    1:200

    Average %

    84

    Range %

    80-96

    1:400

    Average %

    90

    Range %

    83-99

    1:800

    Average %

    87

    Range %

    82-100

    1:1600

    Average %

    85

    Range %

    82-98

  • 回收率：

    The recovery of mouse GHbA1c spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Lysate for RBC (n=5)

    85

    82-95

  • 標準曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    ng/ml

    OD1

    OD2

    Average

    4000

    0.176

    0.184

    0.180

    2000

    0.259

    0.267

    0.263

    1000

    0.363

    0.378

    0.370

    500

    0.520

    0.529

    0.525

    250

    0.794

    0.821

    0.807

    125

    1.174

    1.233

    1.204

    62.5

    1.724

    1.684

    1.704

    0

    2.608

    2.625

    2.616

  • 數據處理：
  • 貨期：
    3-5 working days

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