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Human Glycated hemoglobin A1c,GHbA1c ELISA Kit

  • 中文名稱:
    人糖化血紅蛋白A1c(GHbA1c)酶聯(lián)免疫試劑盒
  • 貨號(hào):
    CSB-E08139h
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥3600/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    人糖化血紅蛋白A1c(GHbA1c)酶聯(lián)免疫試劑盒(CSB-E08139h)為競(jìng)爭法ELISA試劑盒,定量檢測(cè)lysatesforRBC樣本中的GHbA1c含量。GHbA1c即糖化血紅蛋白,它反映過去2-3個(gè)月平均血糖水平。其背景在于可用于糖尿病的診斷、病情監(jiān)測(cè)。研究機(jī)制方面,血糖與血紅蛋白結(jié)合形成糖化血紅蛋白,通過檢測(cè)其水平能評(píng)估血糖控制情況,為治療方案調(diào)整提供依據(jù)。試劑盒檢測(cè)范圍為0.012 ng/mL-50 ng/mL,適用于科研領(lǐng)域?qū)t細(xì)胞裂解液樣本中GHbA1c含量的精準(zhǔn)檢測(cè);在糖尿病病理機(jī)制研究、降糖藥物開發(fā)及紅細(xì)胞代謝分析等方向具有重要科研價(jià)值;適用于體外實(shí)驗(yàn)中對(duì)紅細(xì)胞糖化程度的動(dòng)態(tài)監(jiān)測(cè)、抗糖尿病化合物篩選以及代謝綜合征相關(guān)基礎(chǔ)研究,為探索糖代謝異常與靶器官損傷關(guān)聯(lián)性提供可靠工具。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫:
    GHbA1c
  • 種屬:
    Homo sapiens (Human)
  • 樣本類型:
    lysates for RBC
  • 檢測(cè)范圍:
    0.012 ng/mL-50 ng/mL
  • 靈敏度:
    0.012 ng/mL
  • 反應(yīng)時(shí)間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測(cè)波長:
    450 nm
  • 研究領(lǐng)域:
    Others
  • 測(cè)定原理:
    quantitative
  • 測(cè)定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 標(biāo)準(zhǔn)曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    50 0.270 0.273 0.272    
    12.5 0.367 0.378 0.373    
    3.125 0.463 0.489 0.476    
    0.78 0.560 0.598 0.579    
    0.195 0.692 0.672 0.682    
    0.049 1.384 1.368 1.376    
    0.012 1.994 1.954 1.974    
    0 2.373 2.399 2.386    
  • 本試劑盒所含材料:
    • A 96-well Assay plate --The 96-well plate has been pre-coated with GHbA1c antibody.
    • Standard (10 x concentrate) (1 x 200 μl) --Dilute the standard at dilution series, read the OD values, and then draw a standard curve.
    • Biotin-conjugated GHbA1c (100 x concentrate) (1 x 60 μl)
    • HRP-avidin (100 x concentrate) (1 x 120 μl) --Catalyze the TMB solution, eliciting a chromogemic reaction.
    • Biotin-conjugate Diluent (1 x 10 ml) --Dilute the Biotin-conjugated GHbA1c
    • HRP-avidin Diluent (1 x 20 ml) --Dilute the HRP-avidin
    • Sample Diluent (2 x 20 ml) --Reconstitute the standard and dilute the sample to an appropriate concentration.
    • Wash Buffer (25x concentrate) (1 x 20 ml)--Wash away unbound or free substances.
    • TMB Substrate (1x 10 ml) --Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
    • Stop Solution (1 x 10ml) --Stop the color reaction. The solution color immediately turns from blue to yellow.
    • Four Adhesive Strips (For 96 wells)
    • An Instruction manual

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  • 本試劑盒不含材料:
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm - 570 nm.
    • An incubator that can provide stable incubation conditions up to 37°C±5°C.
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer
    • Absorbent paper for blotting the microtiter plate
    • 50-300ul multi-channel micropipette
    • Pipette tips
    • Single-channel micropipette with different ranges
    • 100ml and 500ml graduated cylinders
    • Deionized or distilled water
    • Timer
    • Test tubes for dilution

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  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

產(chǎn)品評(píng)價(jià)



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