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  4. Mouse Ficolin-2(FCN2) ELISA kit

Mouse Ficolin-2(FCN2) ELISA kit

  • 中文名稱：
    小鼠纖維膠凝蛋白-2(FCN2)酶聯免疫試劑盒
  • 貨號：
    CSB-EL008551MO
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產品詳情

  • 產品描述：
    小鼠纖維膠凝蛋白-2(FCN2)酶聯免疫試劑盒（CSB-EL008551MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的FCN2含量。小鼠纖維膠凝蛋白-2（FCN2）是一種具有唾液酸結合活性的蛋白，參與補體激活和凝集素途徑，在天然免疫應答中發揮重要作用。其功能與血漿膜外成分和膠原三聚體相關，主要在骨髓、胚胎、肝臟和脾臟中表達。試劑盒檢測范圍為125 pg/mL-8000 pg/mL，該產品適用于免疫調節機制、感染性疾病模型及炎癥相關研究，例如探索病原體感染、自身免疫性疾病或代謝紊亂等實驗條件下FCN2的動態表達特征，為小鼠模型中免疫微環境變化提供定量分析工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    Fcn2 ELISA Kit; FcnbFicolin-2 ELISA Kit; Collagen/fibrinogen domain-containing protein 2 ELISA Kit; Ficolin-B ELISA Kit; Ficolin-beta ELISA Kit; L-ficolin ELISA Kit
  • 縮寫：
    FCN2
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    125 pg/mL-8000 pg/mL
  • 靈敏度：
    31.25 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse FCN2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1000 Average % 92
    Range % 88-96
    1:2000 Average % 94
    Range % 90-98
    1:4000 Average % 95
    Range % 90-100
    1:8000 Average % 88
    Range % 83-93
  • 回收率：
    The recovery of mouse FCN2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 84 80-89
    EDTA plasma (n=4) 96 92-101
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    8000 2.495 2.569 2.532 2.385
    4000 2.014 2.071 2.043 1.896
    2000 1.622 1.584 1.603 1.456
    1000 1.178 1.113 1.146 0.999
    500 0.731 0.792 0.762 0.615
    250 0.456 0.428 0.442 0.295
    125 0.345 0.327 0.336 0.189
    0 0.147 0.146 0.147 　
  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    May function in innate immunity through activation of the lectin complement pathway. Calcium-dependent and GlcNAc-binding lectin.
  • 基因功能參考文獻：
    1. this study shows that FCN B plays an important role in the development of collagen Ab-induced arthritis PMID: 28739878
    2. Ficolin-2 defends against virulent Mycobacteria tuberculosis infection in vivo, and its insufficiency is associated with infection in humans. PMID: 24040095
    3. Data show that ficolin-B is stored in and set free from immature granulocytic myeloid cells indicating a role in the early infection-induced cellular response of these inflammatory cells. PMID: 23911405
    4. These results identify mouse ficolin-B as a functional member of the ficolin family activating complement via the lectin pathway. PMID: 22459270
    5. Results show that ficolin B in mouse bone marrow is an oligomeric protein. Ficolin B contained very low, but detectable, amounts of MASP-2 and sMAP and showed detectable C4-deposition activity on immobilized N-acetylglucosamine. PMID: 22523468
    6. Ficolin B exhibited a distinct ontogeny pattern that switched from embryonic liver to postnatal bone marrow and spleen. The cells that express ficolin B mRNA were identified as belonging to the myeloid cell lineage. PMID: 15950722
    7. Ficolin B showed multimeric structures and revealed binding to both N-acetylglucosamine and N-acetylgalactosamine. Ficolin B specifically recognized sialic acid residues. Ficolin B plays a distinct role through its unique carbohydrate-binding specificity. PMID: 16328467

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  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    Ficolin lectin family
  • 數據庫鏈接：


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