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Mouse CD81 antigen(CD81) ELISA kit

  • 中文名稱:
    小鼠CD81抗原(CD81)酶聯免疫試劑盒
  • 貨號:
    CSB-EL004960MO
  • 規格:
    96T/48T
  • 價格:
    ¥3600/¥2500
  • 其他:

產品詳情

  • 產品描述:
    小鼠CD81抗原(CD81)酶聯免疫試劑盒(CSB-EL004960MO)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的CD81含量。CD81 是重要的細胞表面分子,屬于四次跨膜蛋白超家族成員。它參與細胞黏附、遷移及信號傳導等生理過程。在研究機制上,其與多種病毒感染相關,可作為病毒受體,在腫瘤進展、免疫調節等方面的作用也備受關注,是潛在的治療靶點。試劑盒檢測范圍為47 pg/mL-3000 pg/mL,適用于體外研究 CD81 在免疫應答、腫瘤微環境調控或病原體感染模型中的表達動態;支持科研人員高效開展動物模型樣本的定量分析,為探索 CD81 相關分子機制提供可靠工具;尤其在 B 細胞活化、肝臟代謝調控等研究中具有重要價值本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    Cd81 ELISA Kit; Tapa1CD81 antigen ELISA Kit; 26 kDa cell surface protein TAPA-1 ELISA Kit; Target of the antiproliferative ELISA Kit 1 ELISA Kit; CD antigen CD81 ELISA Kit
  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Mus musculus (Mouse)
  • 樣本類型:
    serum, plasma, tissue homogenates, cell lysates
  • 檢測范圍:
    47 pg/mL-3000 pg/mL
  • 靈敏度:
    11.75 pg/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Immunology
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse CD81 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)
    1:1 Average % 97
    Range % 92-101
    1:2 Average % 91
    Range % 85-96
    1:4 Average % 84
    Range % 80-88
    1:8 Average % 95
    Range % 95-99
  • 回收率:
    The recovery of mouse CD81 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 93 89-98
    EDTA plasma (n=4) 104 100-113
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    3000 2.692 2.600 2.646 2.497
    1500 2.057 1.938 1.998 1.849
    750 1.376 1.428 1.402 1.253
    375 0.909 0.854 0.882 0.733
    187.5 0.589 0.609 0.599 0.450
    94 0.365 0.371 0.368 0.219
    47 0.239 0.247 0.243 0.094
    0 0.152 0.145 0.149  
  • 數據處理:
  • 貨期:
    3-5 working days

產品評價

相關問答

 常見問題解答
Q:

我想要將我們期望的蛋白質與外泌體標記物如CD81共定位,以證明該蛋白質定位在外泌體膜上。您認為我是否可以通過使用諸如Mouse CD81 antigen(CD81) ELISA kit (code: CSB-EL004960MO)之類的試劑盒并用一種感興趣的抗蛋白抗體替代檢測抗體來達到這一目的?我可以將此試劑盒用于細胞培養基衍生的外泌體或細胞培養基本身嗎?

A:
試劑盒CSB-EL004960MO用于定量檢測血清、血漿等樣品中CD81的含量。如果您是要分析外泌體樣品(如細胞培養基衍生的外泌體或細胞培養基本身)中該蛋白的含量,理論上可以嘗試用其進行檢測。目前我們這里沒有做過此類檢測,這里建議您安排預實驗。 如果只是做簡單的定位研究,不能準確反映,只能通過內容的變化間接反映是否存在衍生外泌體。

靶點詳情

  • 功能:
    Structural component of specialized membrane microdomains known as tetraspanin-enriched microdomains (TERMs), which act as platforms for receptor clustering and signaling. Essential for trafficking and compartmentalization of CD19 receptor on the cell surface of activated B cells. Upon initial encounter with a microbial pathogen, enables the assembly of CD19-CR2 and B cell receptor complexes at signaling TERMs, lowering the threshold dose of antigen required to trigger B cell clonal expansion and humoral immune response. In T cells, associates with CD4 or CD8 coreceptors and defines the maturation state of antigen-induced synapses with B cells. Facilitates localization of CD3 in these immune synapses, required for costimulation and sustained activation of T cells, preferentially triggering T helper type 2 immune response. Can act both as positive and negative regulator of homotypic or heterotypic cell-cell fusion processes. In myoblasts, associates with another tetraspanin CD9 in complex with PTGFRN and inhibits myotube fusion during muscle regeneration. In macrophages, associates with CD9 and beta-1 and beta-2 integrins, and prevents macrophage fusion into multinucleated giant cells specialized in ingesting complement-opsonized large particles. Also prevents the fusion between mononuclear cell progenitors into osteoclasts in charge of bone resorption. Positively regulates sperm-egg fusion and may be involved in the acrosome reaction. Regulates protein trafficking in intracellular compartments. In T cells, associates with dNTPase SAMHD1 and defines its subcellular location, enabling its degradation by the proteasome and thereby controlling intracellular dNTP levels. Also regulates integrin-dependent migration of macrophages, particularly relevant for inflammatory response in the lung.; (Microbial infection) Specifically required for Plasmodium yoelii infectivity of hepatocytes, controlling sporozoite entry in hepatocytes via the parasitophorous vacuole and subsequent parasite differentiation to exoerythrocytic forms.
  • 基因功能參考文獻:
    1. The species-specific traits in CD9 and CD81 distribution during sperm maturation were compared between mice and humans. A mutual position of CD9/CD81 is shown in human spermatozoa in the acrosomal cap, however in mice, CD9 and CD81 occupy a distinct area. PMID: 29671763
    2. adoptive transfer of wild-type regulatory T cells into CD81-deficient mice was sufficient to promote tumor growth and metastasis; these findings suggested that CD81 modulates adaptive and innate immune responses PMID: 26329536
    3. CD81-Rac interaction exerts an important regulatory role on the innate and adaptive immunity against bacterial infection and suggests a role for CD81 in the development of novel therapeutic targets during infectious diseases. PMID: 25972472
    4. Plasmodium yoelii sporozoite rhoptry discharge occurs only in the presence of CD81, providing the first direct evidence for a role of CD81 during sporozoite productive invasion. PMID: 24798694
    5. In vitro myotubes lacking CD9P-1 or both CD9 and CD81 fuse with a higher frequency than normal myotubes PMID: 23575678
    6. Data suggest that GPC3 down-regulates hepatocyte proliferation by binding to hedgehog (HH) and down-regulating the HH signaling pathway and binding with CD81, thus making it unavailable to bind to Hhex and causing its nuclear translocation. PMID: 23665349
    7. CD81 interacts with the T cell receptor to suppress signaling. PMID: 23226274
    8. CD81 promotes the microvillus formation and/or extension while tetraspanin CD82 inhibits these events. In addition, CD81 enhances the outward bending of the plasma membrane while CD82 inhibits it. PMID: 22079629
    9. Self-renewing hematopoietic stem cells express CD81 during stress-induced proliferation. PMID: 21931533
    10. CD81 is required for the formation of actin membrane protrusions via Rac1 activation in adhesion-dependent immune cell migration. PMID: 21677313
    11. data indicate that CD81 plays a significant role in the final stages of RPE development, controlling cell number and overall developmental pattern PMID: 20882409
    12. CD81 gene is maternally imprinted, with preferential expression from the paternal allele. PMID: 20579434
    13. CD81 plays a negative role in B cell activation in vitro and in vivo. PMID: 19737782
    14. CD81 expression by T cells is critical for their induction of IL-4 synthesis by B cells PMID: 11978781
    15. Infertility of CD9-deficient mouse eggs is reversed by mouse CD81 PMID: 12086470
    16. the role of CD81 in the regulation of astrocyte and microglial number, perhaps by regulating cell proliferation by a contact inhibition-dependent mechanism. PMID: 12357429
    17. Data show that CD81, a putative receptor for hepatitis C virus, is required on hepatocytes for human Plasmodium falciparum and rodent Plasmodium yoelii sporozoite infectivity. PMID: 12483205
    18. CD81 affects expression of its associated partner CD19 beginning at a B-cell postendoplasmic reticulum compartment where CD81 is necessary for normal trafficking or for surface membrane stability of CD19. PMID: 14530327
    19. CD81 strengthens multivalent VLA-4 contacts within subsecond integrin occupancy without altering intrinsic adhesive properties to low density ligand. CD81 facilitates both VLA-4-mediated leukocyte rolling and arrest on VCAM-1 PMID: 14532283
    20. CD81 is required for the association of the B cell antigen receptor and the CD19/CD21 complex with lipid rafts and for enhanced signaling from rafts upon coligation. PMID: 14688345
    21. CD81 signaling events could be mediated by 14-3-3 adapter proteins, and these signals may be dependent on cellular redox PMID: 14966136
    22. CD9 and CD81 play complementary roles in sperm-egg fusion PMID: 16380109
    23. CD81 plays multiple roles in the processing, intracellular trafficking, and membrane functions of CD19. PMID: 16449649
    24. Murine CD81 does not mediate hepatitis C virus pseudoparticle entry when transfected into human HepG2 tumor cells. PMID: 16641285
    25. Disruption of the mouse CD81 gene led to a reduction in the fecundity of female mice, and CD81-/- eggs had impaired ability to fuse with sperm. PMID: 17290409
    26. We conclude that CD81 represents a potential therapeutic target to interfere with leukocyte infiltration and ameliorate inflammatory neurological damage in MS. PMID: 18586096
    27. tetraspanins CD9 and CD81 regulate cell motility and protease production of macrophages and that their dysfunction may underlie the progression of COPD PMID: 18662991
    28. CD81 is indispensable for an authentic in vivo hepatitis C virus infection. PMID: 19030166
    29. CD81 potentially plays a role in macrophage cell line growth regulation. PMID: 19184252
    30. Inactivation of CD81 by homologous recombination can increase astrocyte and microglial cell proliferation and can lead to anomalously large brain size (30% larger than normal). The effect depends on genetic background. PMID: 12357429

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  • 亞細胞定位:
    Cell membrane; Multi-pass membrane protein. Basolateral cell membrane; Multi-pass membrane protein.
  • 蛋白家族:
    Tetraspanin (TM4SF) family
  • 組織特異性:
    Expressed in oocytes (at protein level). Highly expressed in granulosa cells. Expressed in skeletal muscle mainly in endothelial cells of endomysial capillaries, in satellite cells and myoblasts (at protein level). Expressed in hepatocytes (at protein lev
  • 數據庫鏈接:

    KEGG: mmu:12520

    STRING: 10090.ENSMUSP00000043768

    UniGene: Mm.806



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